Data from: Estimation of a killer whale (Orcinus orca) population’s diet using sequencing analysis of DNA from feces
Estimating diet composition is important for understanding interactions between predators and prey and thus illuminating ecosystem function. The diet of many species, however, is difficult to observe directly. Genetic analysis of fecal material collected in the field is therefore a useful tool for g...
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ftdans:oai:easy.dans.knaw.nl:easy-dataset:91502 2023-07-02T03:32:50+02:00 Data from: Estimation of a killer whale (Orcinus orca) population’s diet using sequencing analysis of DNA from feces Ford, Michael J. Hempelmann, Jennifer Hanson, M. Bradley Ayres, Katherine L. Baird, Robin W. Emmons, Candice K. Lundin, Jessica I. Schorr, Gregory S. Wasser, Samuel K. Park, Linda K. 2016-01-25T19:54:55.000+01:00 http://nbn-resolving.org/urn:nbn:nl:ui:13-m4-32jv https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:91502 unknown doi:10.5061/dryad.ds6gc/1 doi:10.1371/journal.pone.0144956 PMID:26735849 http://nbn-resolving.org/urn:nbn:nl:ui:13-m4-32jv doi:10.5061/dryad.ds6gc https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:91502 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Life sciences medicine and health care 2016 ftdans https://doi.org/10.5061/dryad.ds6gc/110.1371/journal.pone.014495610.5061/dryad.ds6gc 2023-06-13T13:20:37Z Estimating diet composition is important for understanding interactions between predators and prey and thus illuminating ecosystem function. The diet of many species, however, is difficult to observe directly. Genetic analysis of fecal material collected in the field is therefore a useful tool for gaining insight into wild animal diets. In this study, we used high-throughput DNA sequencing to quantitatively estimate the diet composition of an endangered population of wild killer whales (Orcinus orca) in their summer range in the Salish Sea. We combined 175 fecal samples collected between May and September from five years between 2006 and 2011 into 13 sample groups. Two known DNA composition control groups were also created. Each group was sequenced at a ~330bp segment of the 16s gene in the mitochondrial genome using an Illumina MiSeq sequencing system. After several quality controls steps, 4,987,107 individual sequences were aligned to a custom sequence database containing 19 potential fish prey species and the most likely species of each fecal-derived sequence was determined. Based on these alignments, salmonids made up >98.6% of the total sequences and thus of the inferred diet. Of the six salmonid species, Chinook salmon made up 79.5% of the sequences, followed by coho salmon (15%). Over all years, a clear pattern emerged with Chinook salmon dominating the estimated diet early in the summer, and coho salmon contributing an average of >40% of the diet in late summer. Sockeye salmon appeared to be occasionally important, at >18% in some sample groups. Non-salmonids were rarely observed. Our results are consistent with earlier results based on surface prey remains, and confirm the importance of Chinook salmon in this population’s summer diet. Other/Unknown Material Killer Whale Orca Orcinus orca Killer whale Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) Sockeye ENVELOPE(-130.143,-130.143,54.160,54.160) |
institution |
Open Polar |
collection |
Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
op_collection_id |
ftdans |
language |
unknown |
topic |
Life sciences medicine and health care |
spellingShingle |
Life sciences medicine and health care Ford, Michael J. Hempelmann, Jennifer Hanson, M. Bradley Ayres, Katherine L. Baird, Robin W. Emmons, Candice K. Lundin, Jessica I. Schorr, Gregory S. Wasser, Samuel K. Park, Linda K. Data from: Estimation of a killer whale (Orcinus orca) population’s diet using sequencing analysis of DNA from feces |
topic_facet |
Life sciences medicine and health care |
description |
Estimating diet composition is important for understanding interactions between predators and prey and thus illuminating ecosystem function. The diet of many species, however, is difficult to observe directly. Genetic analysis of fecal material collected in the field is therefore a useful tool for gaining insight into wild animal diets. In this study, we used high-throughput DNA sequencing to quantitatively estimate the diet composition of an endangered population of wild killer whales (Orcinus orca) in their summer range in the Salish Sea. We combined 175 fecal samples collected between May and September from five years between 2006 and 2011 into 13 sample groups. Two known DNA composition control groups were also created. Each group was sequenced at a ~330bp segment of the 16s gene in the mitochondrial genome using an Illumina MiSeq sequencing system. After several quality controls steps, 4,987,107 individual sequences were aligned to a custom sequence database containing 19 potential fish prey species and the most likely species of each fecal-derived sequence was determined. Based on these alignments, salmonids made up >98.6% of the total sequences and thus of the inferred diet. Of the six salmonid species, Chinook salmon made up 79.5% of the sequences, followed by coho salmon (15%). Over all years, a clear pattern emerged with Chinook salmon dominating the estimated diet early in the summer, and coho salmon contributing an average of >40% of the diet in late summer. Sockeye salmon appeared to be occasionally important, at >18% in some sample groups. Non-salmonids were rarely observed. Our results are consistent with earlier results based on surface prey remains, and confirm the importance of Chinook salmon in this population’s summer diet. |
author |
Ford, Michael J. Hempelmann, Jennifer Hanson, M. Bradley Ayres, Katherine L. Baird, Robin W. Emmons, Candice K. Lundin, Jessica I. Schorr, Gregory S. Wasser, Samuel K. Park, Linda K. |
author_facet |
Ford, Michael J. Hempelmann, Jennifer Hanson, M. Bradley Ayres, Katherine L. Baird, Robin W. Emmons, Candice K. Lundin, Jessica I. Schorr, Gregory S. Wasser, Samuel K. Park, Linda K. |
author_sort |
Ford, Michael J. |
title |
Data from: Estimation of a killer whale (Orcinus orca) population’s diet using sequencing analysis of DNA from feces |
title_short |
Data from: Estimation of a killer whale (Orcinus orca) population’s diet using sequencing analysis of DNA from feces |
title_full |
Data from: Estimation of a killer whale (Orcinus orca) population’s diet using sequencing analysis of DNA from feces |
title_fullStr |
Data from: Estimation of a killer whale (Orcinus orca) population’s diet using sequencing analysis of DNA from feces |
title_full_unstemmed |
Data from: Estimation of a killer whale (Orcinus orca) population’s diet using sequencing analysis of DNA from feces |
title_sort |
data from: estimation of a killer whale (orcinus orca) population’s diet using sequencing analysis of dna from feces |
publishDate |
2016 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-m4-32jv https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:91502 |
long_lat |
ENVELOPE(-130.143,-130.143,54.160,54.160) |
geographic |
Sockeye |
geographic_facet |
Sockeye |
genre |
Killer Whale Orca Orcinus orca Killer whale |
genre_facet |
Killer Whale Orca Orcinus orca Killer whale |
op_relation |
doi:10.5061/dryad.ds6gc/1 doi:10.1371/journal.pone.0144956 PMID:26735849 http://nbn-resolving.org/urn:nbn:nl:ui:13-m4-32jv doi:10.5061/dryad.ds6gc https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:91502 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf |
op_doi |
https://doi.org/10.5061/dryad.ds6gc/110.1371/journal.pone.014495610.5061/dryad.ds6gc |
_version_ |
1770272526997389312 |