Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research
DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area...
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ftdans:oai:easy.dans.knaw.nl:easy-dataset:91486 2023-07-02T03:31:19+02:00 Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, P. Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, V. Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. von Tschirnhaus, M. Roslin, T. 2015-11-18T18:24:46.000+01:00 http://nbn-resolving.org/urn:nbn:nl:ui:13-ug-6j5n https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:91486 unknown doi:10.5061/dryad.sg5s0/1 doi:10.5061/dryad.sg5s0/2 doi:10.5061/dryad.sg5s0/3 doi:10.1111/1755-0998.12489 PMID:26602739 http://nbn-resolving.org/urn:nbn:nl:ui:13-ug-6j5n doi:10.5061/dryad.sg5s0 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:91486 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Life sciences medicine and health care 2015 ftdans https://doi.org/10.5061/dryad.sg5s0/110.5061/dryad.sg5s0/210.5061/dryad.sg5s0/310.1111/1755-0998.1248910.5061/dryad.sg5s0 2023-06-13T13:20:35Z DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community. Other/Unknown Material Arctic Greenland Zackenberg Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) Arctic Greenland |
institution |
Open Polar |
collection |
Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
op_collection_id |
ftdans |
language |
unknown |
topic |
Life sciences medicine and health care |
spellingShingle |
Life sciences medicine and health care Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, P. Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, V. Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. von Tschirnhaus, M. Roslin, T. Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
topic_facet |
Life sciences medicine and health care |
description |
DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community. |
author |
Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, P. Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, V. Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. von Tschirnhaus, M. Roslin, T. |
author_facet |
Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, P. Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, V. Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. von Tschirnhaus, M. Roslin, T. |
author_sort |
Wirta, H. |
title |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_short |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_full |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_fullStr |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_full_unstemmed |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_sort |
data from: establishing a community-wide dna barcode library as a new tool for arctic research |
publishDate |
2015 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-ug-6j5n https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:91486 |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Greenland Zackenberg |
genre_facet |
Arctic Greenland Zackenberg |
op_relation |
doi:10.5061/dryad.sg5s0/1 doi:10.5061/dryad.sg5s0/2 doi:10.5061/dryad.sg5s0/3 doi:10.1111/1755-0998.12489 PMID:26602739 http://nbn-resolving.org/urn:nbn:nl:ui:13-ug-6j5n doi:10.5061/dryad.sg5s0 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:91486 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf |
op_doi |
https://doi.org/10.5061/dryad.sg5s0/110.5061/dryad.sg5s0/210.5061/dryad.sg5s0/310.1111/1755-0998.1248910.5061/dryad.sg5s0 |
_version_ |
1770270701488439296 |