Data from: Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencing
Microalgae in the division Haptophyta play key roles in the marine ecosystem and in global biogeochemical processes. Despite their ecological importance, knowledge on seasonal dynamics, community composition and abundance at the species level is limited due to their small cell size and few morpholog...
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ftdans:oai:easy.dans.knaw.nl:easy-dataset:88379 2023-07-02T03:33:08+02:00 Data from: Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencing Egge, Elianne Sirnæs Johannessen, Torill Vik Andersen, Tom Eikrem, Wenche Bittner, Lucie Larsen, Aud Sandaa, Ruth-Anne Edvardsen, Bente 2015-03-18T15:44:14.000+01:00 http://nbn-resolving.org/urn:nbn:nl:ui:13-t4-ggon https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:88379 unknown doi:10.5061/dryad.b8d21/1 doi:10.5061/dryad.b8d21/2 doi:10.5061/dryad.b8d21/3 doi:10.5061/dryad.b8d21/5 doi:10.5061/dryad.b8d21/6 doi:10.5061/dryad.b8d21/7 doi:10.5061/dryad.b8d21/8 doi:10.1111/mec.13160 PMID:25893259 http://nbn-resolving.org/urn:nbn:nl:ui:13-t4-ggon doi:10.5061/dryad.b8d21 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:88379 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Life sciences medicine and health care 2015 ftdans https://doi.org/10.5061/dryad.b8d21/110.5061/dryad.b8d21/210.5061/dryad.b8d21/310.5061/dryad.b8d21/510.5061/dryad.b8d21/610.5061/dryad.b8d21/710.5061/dryad.b8d21/810.1111/mec.1316010.5061/dryad.b8d21 2023-06-13T13:18:28Z Microalgae in the division Haptophyta play key roles in the marine ecosystem and in global biogeochemical processes. Despite their ecological importance, knowledge on seasonal dynamics, community composition and abundance at the species level is limited due to their small cell size and few morphological features visible under the light microscope. Here, we present unique data on haptophyte seasonal diversity and dynamics from two annual cycles, with the taxonomic resolution and sampling depth obtained with high-throughput sequencing. From outer Oslofjorden, S Norway, nano- and picoplanktonic samples were collected monthly for 2 years, and the haptophytes targeted by amplification of RNA/cDNA with Haptophyta-specific 18S rDNA V4 primers. We obtained 156 operational taxonomic units (OTUs), from c. 400.000 454 pyrosequencing reads, after rigorous bioinformatic filtering and clustering at 99.5%. Most OTUs represented uncultured and/or not yet 18S rDNA-sequenced species. Haptophyte OTU richness and community composition exhibited high temporal variation and significant yearly periodicity. Richness was highest in September–October (autumn) and lowest in April–May (spring). Some taxa were detected all year, such as Chrysochromulina simplex, Emiliania huxleyi and Phaeocystis cordata, whereas most calcifying coccolithophores only appeared from summer to early winter. We also revealed the seasonal dynamics of OTUs representing putative novel classes (clades HAP-3–5) or orders (clades D, E, F). Season, light and temperature accounted for 29% of the variation in OTU composition. Residual variation may be related to biotic factors, such as competition and viral infection. This study provides new, in-depth knowledge on seasonal diversity and dynamics of haptophytes in North Atlantic coastal waters. Other/Unknown Material North Atlantic Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) Norway |
institution |
Open Polar |
collection |
Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
op_collection_id |
ftdans |
language |
unknown |
topic |
Life sciences medicine and health care |
spellingShingle |
Life sciences medicine and health care Egge, Elianne Sirnæs Johannessen, Torill Vik Andersen, Tom Eikrem, Wenche Bittner, Lucie Larsen, Aud Sandaa, Ruth-Anne Edvardsen, Bente Data from: Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencing |
topic_facet |
Life sciences medicine and health care |
description |
Microalgae in the division Haptophyta play key roles in the marine ecosystem and in global biogeochemical processes. Despite their ecological importance, knowledge on seasonal dynamics, community composition and abundance at the species level is limited due to their small cell size and few morphological features visible under the light microscope. Here, we present unique data on haptophyte seasonal diversity and dynamics from two annual cycles, with the taxonomic resolution and sampling depth obtained with high-throughput sequencing. From outer Oslofjorden, S Norway, nano- and picoplanktonic samples were collected monthly for 2 years, and the haptophytes targeted by amplification of RNA/cDNA with Haptophyta-specific 18S rDNA V4 primers. We obtained 156 operational taxonomic units (OTUs), from c. 400.000 454 pyrosequencing reads, after rigorous bioinformatic filtering and clustering at 99.5%. Most OTUs represented uncultured and/or not yet 18S rDNA-sequenced species. Haptophyte OTU richness and community composition exhibited high temporal variation and significant yearly periodicity. Richness was highest in September–October (autumn) and lowest in April–May (spring). Some taxa were detected all year, such as Chrysochromulina simplex, Emiliania huxleyi and Phaeocystis cordata, whereas most calcifying coccolithophores only appeared from summer to early winter. We also revealed the seasonal dynamics of OTUs representing putative novel classes (clades HAP-3–5) or orders (clades D, E, F). Season, light and temperature accounted for 29% of the variation in OTU composition. Residual variation may be related to biotic factors, such as competition and viral infection. This study provides new, in-depth knowledge on seasonal diversity and dynamics of haptophytes in North Atlantic coastal waters. |
author |
Egge, Elianne Sirnæs Johannessen, Torill Vik Andersen, Tom Eikrem, Wenche Bittner, Lucie Larsen, Aud Sandaa, Ruth-Anne Edvardsen, Bente |
author_facet |
Egge, Elianne Sirnæs Johannessen, Torill Vik Andersen, Tom Eikrem, Wenche Bittner, Lucie Larsen, Aud Sandaa, Ruth-Anne Edvardsen, Bente |
author_sort |
Egge, Elianne Sirnæs |
title |
Data from: Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencing |
title_short |
Data from: Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencing |
title_full |
Data from: Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencing |
title_fullStr |
Data from: Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencing |
title_full_unstemmed |
Data from: Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencing |
title_sort |
data from: seasonal diversity and dynamics of haptophytes in the skagerrak, norway, explored by high-throughput sequencing |
publishDate |
2015 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-t4-ggon https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:88379 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
doi:10.5061/dryad.b8d21/1 doi:10.5061/dryad.b8d21/2 doi:10.5061/dryad.b8d21/3 doi:10.5061/dryad.b8d21/5 doi:10.5061/dryad.b8d21/6 doi:10.5061/dryad.b8d21/7 doi:10.5061/dryad.b8d21/8 doi:10.1111/mec.13160 PMID:25893259 http://nbn-resolving.org/urn:nbn:nl:ui:13-t4-ggon doi:10.5061/dryad.b8d21 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:88379 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf |
op_doi |
https://doi.org/10.5061/dryad.b8d21/110.5061/dryad.b8d21/210.5061/dryad.b8d21/310.5061/dryad.b8d21/510.5061/dryad.b8d21/610.5061/dryad.b8d21/710.5061/dryad.b8d21/810.1111/mec.1316010.5061/dryad.b8d21 |
_version_ |
1770272969987194880 |