Data from: Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis
Mosquito-borne infectious diseases are emerging in many regions of the world. Consequently, surveillance of mosquitoes and concomitant infectious agents is of great importance for prediction and prevention of mosquito-borne infectious diseases. Currently, morphological identification of mosquitoes i...
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ftdans:oai:easy.dans.knaw.nl:easy-dataset:84689 2023-07-02T03:33:16+02:00 Data from: Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis Engdahl, Cecilia Larsson, Pär Näslund, Jonas Bravo, Mayra Evander, Magnus Lundstrom, Jan O. Ahlm, Clas Bucht, Göran 2013-11-12T15:16:12.000+01:00 http://nbn-resolving.org/urn:nbn:nl:ui:13-0z-dor3 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:84689 unknown doi:10.5061/dryad.qb4gs/1 doi:10.5061/dryad.qb4gs/2 doi:10.5061/dryad.qb4gs/3 doi:10.1111/1755-0998.12202 PMID:24215491 http://nbn-resolving.org/urn:nbn:nl:ui:13-0z-dor3 doi:10.5061/dryad.qb4gs https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:84689 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Life sciences medicine and health care 2013 ftdans https://doi.org/10.5061/dryad.qb4gs/110.5061/dryad.qb4gs/210.5061/dryad.qb4gs/310.1111/1755-0998.1220210.5061/dryad.qb4gs 2023-06-13T13:11:15Z Mosquito-borne infectious diseases are emerging in many regions of the world. Consequently, surveillance of mosquitoes and concomitant infectious agents is of great importance for prediction and prevention of mosquito-borne infectious diseases. Currently, morphological identification of mosquitoes is the traditional procedure. However, sequencing of specified genes or standard genomic regions, DNA barcoding, has recently been suggested as a global standard for identification and classification of many different species. Our aim was to develop a genetic method to identify mosquitoes and to study their relationship. Mosquitoes were captured at collection sites in northern Sweden and identified morphologically before the cytochrome c oxidase subunit I (COI) gene sequences of 14 of the most common mosquito species were determined. The sequences obtained were then used for phylogenetic placement, for validation and benchmarking of phenetic classifications, and finally to develop a hierarchical PCR-based typing scheme based on single nucleotide polymorphism sites (SNPs) to enable rapid genetic identification, circumventing the need for morphological characterization. The results showed that exact phylogenetic relationships between mosquito taxa were preserved at shorter evolutionary distances, but at deeper levels they could not be inferred with confidence by using COI gene sequence data alone. Fourteen of the most common mosquito species in Sweden were identified by the SNP/PCR-based typing scheme, demonstrating that genetic typing using SNPs of the COI gene is a useful method for identification of mosquitoes with potential for worldwide application. Other/Unknown Material Northern Sweden Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
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Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
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Life sciences medicine and health care |
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Life sciences medicine and health care Engdahl, Cecilia Larsson, Pär Näslund, Jonas Bravo, Mayra Evander, Magnus Lundstrom, Jan O. Ahlm, Clas Bucht, Göran Data from: Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis |
topic_facet |
Life sciences medicine and health care |
description |
Mosquito-borne infectious diseases are emerging in many regions of the world. Consequently, surveillance of mosquitoes and concomitant infectious agents is of great importance for prediction and prevention of mosquito-borne infectious diseases. Currently, morphological identification of mosquitoes is the traditional procedure. However, sequencing of specified genes or standard genomic regions, DNA barcoding, has recently been suggested as a global standard for identification and classification of many different species. Our aim was to develop a genetic method to identify mosquitoes and to study their relationship. Mosquitoes were captured at collection sites in northern Sweden and identified morphologically before the cytochrome c oxidase subunit I (COI) gene sequences of 14 of the most common mosquito species were determined. The sequences obtained were then used for phylogenetic placement, for validation and benchmarking of phenetic classifications, and finally to develop a hierarchical PCR-based typing scheme based on single nucleotide polymorphism sites (SNPs) to enable rapid genetic identification, circumventing the need for morphological characterization. The results showed that exact phylogenetic relationships between mosquito taxa were preserved at shorter evolutionary distances, but at deeper levels they could not be inferred with confidence by using COI gene sequence data alone. Fourteen of the most common mosquito species in Sweden were identified by the SNP/PCR-based typing scheme, demonstrating that genetic typing using SNPs of the COI gene is a useful method for identification of mosquitoes with potential for worldwide application. |
author |
Engdahl, Cecilia Larsson, Pär Näslund, Jonas Bravo, Mayra Evander, Magnus Lundstrom, Jan O. Ahlm, Clas Bucht, Göran |
author_facet |
Engdahl, Cecilia Larsson, Pär Näslund, Jonas Bravo, Mayra Evander, Magnus Lundstrom, Jan O. Ahlm, Clas Bucht, Göran |
author_sort |
Engdahl, Cecilia |
title |
Data from: Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis |
title_short |
Data from: Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis |
title_full |
Data from: Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis |
title_fullStr |
Data from: Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis |
title_full_unstemmed |
Data from: Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis |
title_sort |
data from: identification of swedish mosquitoes based on molecular barcoding of the coi gene and snp analysis |
publishDate |
2013 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-0z-dor3 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:84689 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
doi:10.5061/dryad.qb4gs/1 doi:10.5061/dryad.qb4gs/2 doi:10.5061/dryad.qb4gs/3 doi:10.1111/1755-0998.12202 PMID:24215491 http://nbn-resolving.org/urn:nbn:nl:ui:13-0z-dor3 doi:10.5061/dryad.qb4gs https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:84689 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf |
op_doi |
https://doi.org/10.5061/dryad.qb4gs/110.5061/dryad.qb4gs/210.5061/dryad.qb4gs/310.1111/1755-0998.1220210.5061/dryad.qb4gs |
_version_ |
1770273128390328320 |