Data from: A molecular gut content study of Themisto abyssorum (Amphipoda) from Arctic hydrothermal vent and cold seep systems
The use of DNA as a marker for prey inside the gut of predators has been instrumental in further understanding of known and unknown interactions. Molecular approaches are in particular useful in unavailable environments like the deep-sea. Trophic interactions in the deep-sea are difficult to observe...
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ftdans:oai:easy.dans.knaw.nl:easy-dataset:84377 2023-07-02T03:31:32+02:00 Data from: A molecular gut content study of Themisto abyssorum (Amphipoda) from Arctic hydrothermal vent and cold seep systems Olsen, Bernt Rydland Troedsson, Christofer Hadziavdic, Kenan Rapp, Hans Tore Pedersen, Rolf B. 2013-09-10T19:13:26.000+02:00 http://nbn-resolving.org/urn:nbn:nl:ui:13-qd-bfip https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:84377 unknown doi:10.5061/dryad.tm1k0/1 doi:10.5061/dryad.tm1k0/2 doi:10.5061/dryad.tm1k0/3 doi:10.5061/dryad.tm1k0/4 doi:10.5061/dryad.tm1k0/5 doi:10.5061/dryad.tm1k0/6 doi:10.5061/dryad.tm1k0/8 doi:10.5061/dryad.tm1k0/7 doi:10.1111/mec.12511 PMID:24172025 http://nbn-resolving.org/urn:nbn:nl:ui:13-qd-bfip doi:10.5061/dryad.tm1k0 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:84377 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Life sciences medicine and health care 2013 ftdans https://doi.org/10.5061/dryad.tm1k0/110.5061/dryad.tm1k0/210.5061/dryad.tm1k0/310.5061/dryad.tm1k0/410.5061/dryad.tm1k0/510.5061/dryad.tm1k0/610.5061/dryad.tm1k0/810.5061/dryad.tm1k0/710.1111/mec.1251110.5061/dryad.tm1k0 2023-06-13T13:10:47Z The use of DNA as a marker for prey inside the gut of predators has been instrumental in further understanding of known and unknown interactions. Molecular approaches are in particular useful in unavailable environments like the deep-sea. Trophic interactions in the deep-sea are difficult to observe in situ, correct deep-sea experimental laboratory conditions are difficult to obtain, animals rarely survive the sampling, or the study organisms feed during the sampling due to long hauls. Preliminary studies of vent and seep systems in the Nordic Seas have identified the temperate-cold water pelagic amphipod Themisto abyssorum as a potentially important predator these chemosynthetic habitats. However, the prey of this deep-sea predator is poorly known, and we applied Denaturing High Performance Liquid Chromatography (DHPLC) to investigate the predator - prey interactions of T. abyssorum in deep-water vent and seep systems. Two deep-water hydrothermally active localities (The Jan Mayen and Loki's Castle vent fields) and one cold seep locality (The Håkon Mosby mud volcano) in the Nordic Seas were sampled, genomic DNA of the stomachs of T. abyssorum was extracted, and 18S rDNA gene was amplified and used to map the stomach content. We found a wide range of organisms including micro-eukaryotes, metazoans and detritus. Themisto abyssorum specimens from Loki's Castle had the highest diversity of prey. The wide range of prey items found suggests that T. abyssorum might be involved in more than one trophic level and should be regarded as an omnivore and not a strict carnivore as have previously been suggested. Other/Unknown Material Arctic Jan Mayen Nordic Seas Themisto abyssorum Themisto Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) Arctic Jan Mayen |
institution |
Open Polar |
collection |
Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
op_collection_id |
ftdans |
language |
unknown |
topic |
Life sciences medicine and health care |
spellingShingle |
Life sciences medicine and health care Olsen, Bernt Rydland Troedsson, Christofer Hadziavdic, Kenan Rapp, Hans Tore Pedersen, Rolf B. Data from: A molecular gut content study of Themisto abyssorum (Amphipoda) from Arctic hydrothermal vent and cold seep systems |
topic_facet |
Life sciences medicine and health care |
description |
The use of DNA as a marker for prey inside the gut of predators has been instrumental in further understanding of known and unknown interactions. Molecular approaches are in particular useful in unavailable environments like the deep-sea. Trophic interactions in the deep-sea are difficult to observe in situ, correct deep-sea experimental laboratory conditions are difficult to obtain, animals rarely survive the sampling, or the study organisms feed during the sampling due to long hauls. Preliminary studies of vent and seep systems in the Nordic Seas have identified the temperate-cold water pelagic amphipod Themisto abyssorum as a potentially important predator these chemosynthetic habitats. However, the prey of this deep-sea predator is poorly known, and we applied Denaturing High Performance Liquid Chromatography (DHPLC) to investigate the predator - prey interactions of T. abyssorum in deep-water vent and seep systems. Two deep-water hydrothermally active localities (The Jan Mayen and Loki's Castle vent fields) and one cold seep locality (The Håkon Mosby mud volcano) in the Nordic Seas were sampled, genomic DNA of the stomachs of T. abyssorum was extracted, and 18S rDNA gene was amplified and used to map the stomach content. We found a wide range of organisms including micro-eukaryotes, metazoans and detritus. Themisto abyssorum specimens from Loki's Castle had the highest diversity of prey. The wide range of prey items found suggests that T. abyssorum might be involved in more than one trophic level and should be regarded as an omnivore and not a strict carnivore as have previously been suggested. |
author |
Olsen, Bernt Rydland Troedsson, Christofer Hadziavdic, Kenan Rapp, Hans Tore Pedersen, Rolf B. |
author_facet |
Olsen, Bernt Rydland Troedsson, Christofer Hadziavdic, Kenan Rapp, Hans Tore Pedersen, Rolf B. |
author_sort |
Olsen, Bernt Rydland |
title |
Data from: A molecular gut content study of Themisto abyssorum (Amphipoda) from Arctic hydrothermal vent and cold seep systems |
title_short |
Data from: A molecular gut content study of Themisto abyssorum (Amphipoda) from Arctic hydrothermal vent and cold seep systems |
title_full |
Data from: A molecular gut content study of Themisto abyssorum (Amphipoda) from Arctic hydrothermal vent and cold seep systems |
title_fullStr |
Data from: A molecular gut content study of Themisto abyssorum (Amphipoda) from Arctic hydrothermal vent and cold seep systems |
title_full_unstemmed |
Data from: A molecular gut content study of Themisto abyssorum (Amphipoda) from Arctic hydrothermal vent and cold seep systems |
title_sort |
data from: a molecular gut content study of themisto abyssorum (amphipoda) from arctic hydrothermal vent and cold seep systems |
publishDate |
2013 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-qd-bfip https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:84377 |
geographic |
Arctic Jan Mayen |
geographic_facet |
Arctic Jan Mayen |
genre |
Arctic Jan Mayen Nordic Seas Themisto abyssorum Themisto |
genre_facet |
Arctic Jan Mayen Nordic Seas Themisto abyssorum Themisto |
op_relation |
doi:10.5061/dryad.tm1k0/1 doi:10.5061/dryad.tm1k0/2 doi:10.5061/dryad.tm1k0/3 doi:10.5061/dryad.tm1k0/4 doi:10.5061/dryad.tm1k0/5 doi:10.5061/dryad.tm1k0/6 doi:10.5061/dryad.tm1k0/8 doi:10.5061/dryad.tm1k0/7 doi:10.1111/mec.12511 PMID:24172025 http://nbn-resolving.org/urn:nbn:nl:ui:13-qd-bfip doi:10.5061/dryad.tm1k0 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:84377 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf |
op_doi |
https://doi.org/10.5061/dryad.tm1k0/110.5061/dryad.tm1k0/210.5061/dryad.tm1k0/310.5061/dryad.tm1k0/410.5061/dryad.tm1k0/510.5061/dryad.tm1k0/610.5061/dryad.tm1k0/810.5061/dryad.tm1k0/710.1111/mec.1251110.5061/dryad.tm1k0 |
_version_ |
1770270905806618624 |