Data from: Browsed twig environmental DNA: diagnostic PCR to identify ungulate species
Ungulate browsing can have a strong effect on ecological processes by affecting plant community structure and composition, with cascading effects on nutrient cycling and animal communities. However, in the absence of direct observations of foraging, species-specific foraging behaviors are difficult...
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ftdans:oai:easy.dans.knaw.nl:easy-dataset:82097 2023-07-02T03:29:31+02:00 Data from: Browsed twig environmental DNA: diagnostic PCR to identify ungulate species Nichols, Ruth V. Königsson, Helena Danell, Kjell Spong, Göran 2012-06-21T18:44:29.000+02:00 http://nbn-resolving.org/urn:nbn:nl:ui:13-f3-7nst https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:82097 unknown doi:10.5061/dryad.3nh92/1 doi:10.1111/j.1755-0998.2012.03172.x PMID:22813481 http://nbn-resolving.org/urn:nbn:nl:ui:13-f3-7nst doi:10.5061/dryad.3nh92 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:82097 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Life sciences medicine and health care 2012 ftdans https://doi.org/10.5061/dryad.3nh92/110.1111/j.1755-0998.2012.03172.x10.5061/dryad.3nh92 2023-06-13T13:04:09Z Ungulate browsing can have a strong effect on ecological processes by affecting plant community structure and composition, with cascading effects on nutrient cycling and animal communities. However, in the absence of direct observations of foraging, species-specific foraging behaviors are difficult to quantify. We therefore know relatively little about foraging competition and species specific browsing patterns in systems with several browsers. However, during browsing, a small amount of saliva containing buccal cells is deposited at the bite site, providing a source of environmental DNA (eDNA) that can be used for species identification. Here we describe extraction and PCR protocols for a browser species diagnostic kit. Species specific primers for mitochondrial DNA were optimized and validated using twigs browsed by captive animals. A time series showed that about 50% of the samples will amplify up to 12 weeks after the browsing event, and that some samples amplify up to 24 weeks after browsing (12.5%). Applied to samples of natural browsing from an area where moose (Alces alces), roe deer (Capreolus capreolus), fallow deer (Cervus dama), and red deer (Cervus elaphus) are sympatric, amplification success reached 75%. This method promises to greatly improve our understanding of multispecies browsing systems without the need for direct observations. Other/Unknown Material Alces alces Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
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Open Polar |
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Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
op_collection_id |
ftdans |
language |
unknown |
topic |
Life sciences medicine and health care |
spellingShingle |
Life sciences medicine and health care Nichols, Ruth V. Königsson, Helena Danell, Kjell Spong, Göran Data from: Browsed twig environmental DNA: diagnostic PCR to identify ungulate species |
topic_facet |
Life sciences medicine and health care |
description |
Ungulate browsing can have a strong effect on ecological processes by affecting plant community structure and composition, with cascading effects on nutrient cycling and animal communities. However, in the absence of direct observations of foraging, species-specific foraging behaviors are difficult to quantify. We therefore know relatively little about foraging competition and species specific browsing patterns in systems with several browsers. However, during browsing, a small amount of saliva containing buccal cells is deposited at the bite site, providing a source of environmental DNA (eDNA) that can be used for species identification. Here we describe extraction and PCR protocols for a browser species diagnostic kit. Species specific primers for mitochondrial DNA were optimized and validated using twigs browsed by captive animals. A time series showed that about 50% of the samples will amplify up to 12 weeks after the browsing event, and that some samples amplify up to 24 weeks after browsing (12.5%). Applied to samples of natural browsing from an area where moose (Alces alces), roe deer (Capreolus capreolus), fallow deer (Cervus dama), and red deer (Cervus elaphus) are sympatric, amplification success reached 75%. This method promises to greatly improve our understanding of multispecies browsing systems without the need for direct observations. |
author |
Nichols, Ruth V. Königsson, Helena Danell, Kjell Spong, Göran |
author_facet |
Nichols, Ruth V. Königsson, Helena Danell, Kjell Spong, Göran |
author_sort |
Nichols, Ruth V. |
title |
Data from: Browsed twig environmental DNA: diagnostic PCR to identify ungulate species |
title_short |
Data from: Browsed twig environmental DNA: diagnostic PCR to identify ungulate species |
title_full |
Data from: Browsed twig environmental DNA: diagnostic PCR to identify ungulate species |
title_fullStr |
Data from: Browsed twig environmental DNA: diagnostic PCR to identify ungulate species |
title_full_unstemmed |
Data from: Browsed twig environmental DNA: diagnostic PCR to identify ungulate species |
title_sort |
data from: browsed twig environmental dna: diagnostic pcr to identify ungulate species |
publishDate |
2012 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-f3-7nst https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:82097 |
genre |
Alces alces |
genre_facet |
Alces alces |
op_relation |
doi:10.5061/dryad.3nh92/1 doi:10.1111/j.1755-0998.2012.03172.x PMID:22813481 http://nbn-resolving.org/urn:nbn:nl:ui:13-f3-7nst doi:10.5061/dryad.3nh92 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:82097 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf |
op_doi |
https://doi.org/10.5061/dryad.3nh92/110.1111/j.1755-0998.2012.03172.x10.5061/dryad.3nh92 |
_version_ |
1770274855704330240 |