Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data
We generated ringed seal (Phoca hispida) induced multipotent stem cells from fibroblasts by overexpression of human transgenes: OCT4, SOX2, KLF4 and c-MYC. The resulting cell line, iPHIS1, had the expression of pluripotency marker gene Rex1. Differentiation in embryoid body-like structures allowed u...
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ftdans:oai:easy.dans.knaw.nl:easy-dataset:207128 2023-07-02T03:33:27+02:00 Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data Menzorov, A (via Mendeley Data) 2021-04-09T08:27:25.149Z http://nbn-resolving.org/urn:nbn:nl:ui:13-4h-smht https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:207128 unknown 2 5mkvk5yc4w http://nbn-resolving.org/urn:nbn:nl:ui:13-4h-smht doi:10.17632/5mkvk5yc4w.2 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:207128 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Aleksei Menzorov Interdisciplinary sciences 2021 ftdans https://doi.org/10.17632/5mkvk5yc4w.2 2023-06-13T13:17:35Z We generated ringed seal (Phoca hispida) induced multipotent stem cells from fibroblasts by overexpression of human transgenes: OCT4, SOX2, KLF4 and c-MYC. The resulting cell line, iPHIS1, had the expression of pluripotency marker gene Rex1. Differentiation in embryoid body-like structures allowed us to register expression of AFP, endoderm marker, and Cdx2, trophectoderm marker, but not neuronal (ectoderm) markers. The cells readily differentiated into adipocytes and osteocytes, mesoderm cell types of origin. Transcriptome analysis allowed us to conclude that the cell line does not resemble human pluripotent cells, and, therefore, most probably are not pluripotent. Thus, we produced ringed seal multipotent stem cell line capable of differentiation into adipo- and osteocytes. The raw sequence data are available in NCBI BioProject repository, accession number: PRJNA718133, link: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA718133. The data below represents transcriptome analysis. Other/Unknown Material Phoca hispida ringed seal Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
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Open Polar |
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Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
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ftdans |
language |
unknown |
topic |
Interdisciplinary sciences |
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Interdisciplinary sciences Menzorov, A (via Mendeley Data) Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data |
topic_facet |
Interdisciplinary sciences |
description |
We generated ringed seal (Phoca hispida) induced multipotent stem cells from fibroblasts by overexpression of human transgenes: OCT4, SOX2, KLF4 and c-MYC. The resulting cell line, iPHIS1, had the expression of pluripotency marker gene Rex1. Differentiation in embryoid body-like structures allowed us to register expression of AFP, endoderm marker, and Cdx2, trophectoderm marker, but not neuronal (ectoderm) markers. The cells readily differentiated into adipocytes and osteocytes, mesoderm cell types of origin. Transcriptome analysis allowed us to conclude that the cell line does not resemble human pluripotent cells, and, therefore, most probably are not pluripotent. Thus, we produced ringed seal multipotent stem cell line capable of differentiation into adipo- and osteocytes. The raw sequence data are available in NCBI BioProject repository, accession number: PRJNA718133, link: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA718133. The data below represents transcriptome analysis. |
author |
Menzorov, A (via Mendeley Data) |
author_facet |
Menzorov, A (via Mendeley Data) |
author_sort |
Menzorov, A (via Mendeley Data) |
title |
Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data |
title_short |
Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data |
title_full |
Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data |
title_fullStr |
Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data |
title_full_unstemmed |
Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data |
title_sort |
ringed seal (phoca hispida) induced multipotent stem cells transcriptome analysis data |
publishDate |
2021 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-4h-smht https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:207128 |
genre |
Phoca hispida ringed seal |
genre_facet |
Phoca hispida ringed seal |
op_relation |
2 5mkvk5yc4w http://nbn-resolving.org/urn:nbn:nl:ui:13-4h-smht doi:10.17632/5mkvk5yc4w.2 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:207128 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Aleksei Menzorov |
op_doi |
https://doi.org/10.17632/5mkvk5yc4w.2 |
_version_ |
1770273420475367424 |