Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data

• Main Author: Menzorov, A (via Mendeley Data)
• Language: unknown
• Published: 2021
• Subjects: Interdisciplinary sciences; Phoca hispida; ringed seal
• Online Access: http://nbn-resolving.org/urn:nbn:nl:ui:13-49-0ggi; https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:207097

We generated ringed seal (Phoca hispida) induced multipotent stem cells from fibroblasts by overexpression of human transgenes: OCT4, SOX2, KLF4 and c-MYC. The resulting cell line, iPHIS1, had the expression of pluripotency marker gene Rex1. Differentiation in embryoid body-like structures allowed us to register expression of AFP, endoderm marker, and Cdx2, trophectoderm marker, but not neuronal (ectoderm) markers. The cells readily differentiated into adipocytes and osteocytes, mesoderm cell types of origin. Transcriptome analysis allowed us to conclude that the cell line does not resemble human pluripotent cells, and, therefore, most probably are not pluripotent. Thus, we produced ringed seal multipotent stem cell line capable of differentiation into adipo- and osteocytes. The raw sequence data are available in NCBI BioProject repository, accession number: PRJNA718133, link: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA718133. The data below represents transcriptome analysis.