Data from: Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance
Disruption of neuronal function is likely to influence limits to thermal tolerance. We hypothesized that with acute warming the structure and function of neuronal membranes in the Antarctic notothenioid fish Chaenocephalus aceratus are more vulnerable to perturbation than membranes in the more therm...
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ftdans:oai:easy.dans.knaw.nl:easy-dataset:120224 2023-07-02T03:30:18+02:00 Data from: Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance Biederman, Amanda M. Kuhn, Donald E. O'Brien, Kristin M. Crockett, Elizabeth L 2019-02-12T21:00:04.000+01:00 http://nbn-resolving.org/urn:nbn:nl:ui:13-12-2bow https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:120224 unknown doi:10.5061/dryad.qm0b25h/1 doi:10.5061/dryad.qm0b25h/2 doi:10.5061/dryad.qm0b25h/3 doi:10.5061/dryad.qm0b25h/4 doi:10.5061/dryad.qm0b25h/5 doi:10.5061/dryad.qm0b25h/6 doi:10.5061/dryad.qm0b25h/7 doi:10.5061/dryad.qm0b25h/8 doi:10.5061/dryad.qm0b25h/9 doi:10.5061/dryad.qm0b25h/10 doi:10.5061/dryad.qm0b25h/11 doi:10.1007/s00360-019-01207-x http://nbn-resolving.org/urn:nbn:nl:ui:13-12-2bow doi:10.5061/dryad.qm0b25h https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:120224 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Life sciences medicine and health care 2019 ftdans https://doi.org/10.5061/dryad.qm0b25h/110.5061/dryad.qm0b25h/210.5061/dryad.qm0b25h/310.5061/dryad.qm0b25h/410.5061/dryad.qm0b25h/510.5061/dryad.qm0b25h/610.5061/dryad.qm0b25h/710.5061/dryad.qm0b25h/810.5061/dryad.qm0b25h/910.5061/dryad.qm0b25h/1010.5061/ 2023-06-13T13:35:24Z Disruption of neuronal function is likely to influence limits to thermal tolerance. We hypothesized that with acute warming the structure and function of neuronal membranes in the Antarctic notothenioid fish Chaenocephalus aceratus are more vulnerable to perturbation than membranes in the more thermotolerant notothenioid Notothenia coriiceps. Fluidity was quantified in synaptic membranes, mitochondrial membranes, and myelin from brains of both species of Antarctic fishes. Polar lipid compositions and cholesterol contents were analyzed in myelin; cholesterol was measured in synaptic membranes. Thermal profiles were determined for activities of two membrane-associated proteins, acetylcholinesterase (AChE) and Na+/K+-ATPase (NKA), from brains of animals maintained at ambient temperature or exposed to their critical thermal maxima (CTMAX). Synaptic membranes of C. aceratus were consistently more fluid than those of N. coriiceps (P < 0.0001). Although the fluidities of both myelin and mitochondrial membranes were similar among species, sensitivity of myelin fluidity to in vitro warming was greater in N. coriiceps than in C. aceratus (P < 0.001), which can be explained by lower cholesterol contents in myelin of N. coriiceps (P < 0.05). Activities of both enzymes, AChE and NKA, declined upon CTMAX exposure in C. aceratus, but not in N. coriiceps. We suggest that hyper-fluidization of synaptic membranes with warming in C. aceratus may explain the greater stenothermy in this species, and that thermal limits in notothenioids are more likely to be influenced by perturbations in synaptic membranes than in other membranes of the nervous system. Other/Unknown Material Antarc* Antarctic Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) Antarctic The Antarctic Journal of the Atmospheric Sciences 69 11 3433 3448 |
institution |
Open Polar |
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Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
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ftdans |
language |
unknown |
topic |
Life sciences medicine and health care |
spellingShingle |
Life sciences medicine and health care Biederman, Amanda M. Kuhn, Donald E. O'Brien, Kristin M. Crockett, Elizabeth L Data from: Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance |
topic_facet |
Life sciences medicine and health care |
description |
Disruption of neuronal function is likely to influence limits to thermal tolerance. We hypothesized that with acute warming the structure and function of neuronal membranes in the Antarctic notothenioid fish Chaenocephalus aceratus are more vulnerable to perturbation than membranes in the more thermotolerant notothenioid Notothenia coriiceps. Fluidity was quantified in synaptic membranes, mitochondrial membranes, and myelin from brains of both species of Antarctic fishes. Polar lipid compositions and cholesterol contents were analyzed in myelin; cholesterol was measured in synaptic membranes. Thermal profiles were determined for activities of two membrane-associated proteins, acetylcholinesterase (AChE) and Na+/K+-ATPase (NKA), from brains of animals maintained at ambient temperature or exposed to their critical thermal maxima (CTMAX). Synaptic membranes of C. aceratus were consistently more fluid than those of N. coriiceps (P < 0.0001). Although the fluidities of both myelin and mitochondrial membranes were similar among species, sensitivity of myelin fluidity to in vitro warming was greater in N. coriiceps than in C. aceratus (P < 0.001), which can be explained by lower cholesterol contents in myelin of N. coriiceps (P < 0.05). Activities of both enzymes, AChE and NKA, declined upon CTMAX exposure in C. aceratus, but not in N. coriiceps. We suggest that hyper-fluidization of synaptic membranes with warming in C. aceratus may explain the greater stenothermy in this species, and that thermal limits in notothenioids are more likely to be influenced by perturbations in synaptic membranes than in other membranes of the nervous system. |
author |
Biederman, Amanda M. Kuhn, Donald E. O'Brien, Kristin M. Crockett, Elizabeth L |
author_facet |
Biederman, Amanda M. Kuhn, Donald E. O'Brien, Kristin M. Crockett, Elizabeth L |
author_sort |
Biederman, Amanda M. |
title |
Data from: Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance |
title_short |
Data from: Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance |
title_full |
Data from: Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance |
title_fullStr |
Data from: Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance |
title_full_unstemmed |
Data from: Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance |
title_sort |
data from: physical, chemical, and functional properties of neuronal membranes vary between species of antarctic notothenioids differing in thermal tolerance |
publishDate |
2019 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-12-2bow https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:120224 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
doi:10.5061/dryad.qm0b25h/1 doi:10.5061/dryad.qm0b25h/2 doi:10.5061/dryad.qm0b25h/3 doi:10.5061/dryad.qm0b25h/4 doi:10.5061/dryad.qm0b25h/5 doi:10.5061/dryad.qm0b25h/6 doi:10.5061/dryad.qm0b25h/7 doi:10.5061/dryad.qm0b25h/8 doi:10.5061/dryad.qm0b25h/9 doi:10.5061/dryad.qm0b25h/10 doi:10.5061/dryad.qm0b25h/11 doi:10.1007/s00360-019-01207-x http://nbn-resolving.org/urn:nbn:nl:ui:13-12-2bow doi:10.5061/dryad.qm0b25h https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:120224 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf |
op_doi |
https://doi.org/10.5061/dryad.qm0b25h/110.5061/dryad.qm0b25h/210.5061/dryad.qm0b25h/310.5061/dryad.qm0b25h/410.5061/dryad.qm0b25h/510.5061/dryad.qm0b25h/610.5061/dryad.qm0b25h/710.5061/dryad.qm0b25h/810.5061/dryad.qm0b25h/910.5061/dryad.qm0b25h/1010.5061/ |
container_title |
Journal of the Atmospheric Sciences |
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69 |
container_issue |
11 |
container_start_page |
3433 |
op_container_end_page |
3448 |
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1770274519618945024 |