Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas
Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionised calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly und...
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ftdans:oai:easy.dans.knaw.nl:easy-dataset:113704 2023-07-02T03:32:03+02:00 Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas Sillanpää, Jenni Sundh, Henrik Sundell, Kristina 2018-10-25T15:58:03.000+02:00 http://nbn-resolving.org/urn:nbn:nl:ui:13-ia-2mcn https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:113704 unknown doi:10.5061/dryad.kn45n49/1 doi:10.5061/dryad.kn45n49/2 doi:10.5061/dryad.kn45n49/3 doi:10.5061/dryad.kn45n49/4 doi:10.5061/dryad.kn45n49/5 doi:10.5061/dryad.kn45n49/6 doi:10.5061/dryad.kn45n49/7 doi:10.5061/dryad.kn45n49/8 doi:10.5061/dryad.kn45n49/9 doi:10.5061/dryad.kn45n49/10 doi:10.5061/dryad.kn45n49/11 doi:10.5061/dryad.kn45n49/12 10.1098/rspb http://nbn-resolving.org/urn:nbn:nl:ui:13-ia-2mcn doi:10.5061/dryad.kn45n49 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:113704 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Life sciences medicine and health care 2018 ftdans https://doi.org/10.5061/dryad.kn45n49/110.5061/dryad.kn45n49/210.5061/dryad.kn45n49/310.5061/dryad.kn45n49/410.5061/dryad.kn45n49/510.5061/dryad.kn45n49/610.5061/dryad.kn45n49/710.5061/dryad.kn45n49/810.5061/dryad.kn45n49/910.5061/dryad.kn45n49/1010.5061/ 2023-06-13T12:39:14Z Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionised calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterise the Ca2+ transfer performed by the OME of the pacific oyster, Crassostrea gigas, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca2+ transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis-Menten kinetics and is characterised by a Km of 6.2 mM and a Vmax of 3.3 nM min-1. The transcellular transfer of Ca2+ accounts for approximately 60 % of the total Ca2+ transfer across the OME of C. gigas at environmental Ca2+ concentrations. The use of the pharmacological inhibitors: verapamil, ouabain and caloxin 1a1 revealed that voltage-gated Ca2+-channels, plasma-membrane Ca2+-ATPase and Na+/Ca2+-exchanger all participate in the transcellular Ca2+ transfer across the OME and a model for this Ca2+ transfer is presented and discussed. Other/Unknown Material Crassostrea gigas Pacific oyster Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) Pacific Journal of Oleo Science 59 6 281 292 |
institution |
Open Polar |
collection |
Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
op_collection_id |
ftdans |
language |
unknown |
topic |
Life sciences medicine and health care |
spellingShingle |
Life sciences medicine and health care Sillanpää, Jenni Sundh, Henrik Sundell, Kristina Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
topic_facet |
Life sciences medicine and health care |
description |
Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionised calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterise the Ca2+ transfer performed by the OME of the pacific oyster, Crassostrea gigas, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca2+ transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis-Menten kinetics and is characterised by a Km of 6.2 mM and a Vmax of 3.3 nM min-1. The transcellular transfer of Ca2+ accounts for approximately 60 % of the total Ca2+ transfer across the OME of C. gigas at environmental Ca2+ concentrations. The use of the pharmacological inhibitors: verapamil, ouabain and caloxin 1a1 revealed that voltage-gated Ca2+-channels, plasma-membrane Ca2+-ATPase and Na+/Ca2+-exchanger all participate in the transcellular Ca2+ transfer across the OME and a model for this Ca2+ transfer is presented and discussed. |
author |
Sillanpää, Jenni Sundh, Henrik Sundell, Kristina |
author_facet |
Sillanpää, Jenni Sundh, Henrik Sundell, Kristina |
author_sort |
Sillanpää, Jenni |
title |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_short |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_full |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_sort |
data from: calcium transfer across the outer mantle epithelium in the pacific oyster, crassostrea gigas |
publishDate |
2018 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-ia-2mcn https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:113704 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.5061/dryad.kn45n49/1 doi:10.5061/dryad.kn45n49/2 doi:10.5061/dryad.kn45n49/3 doi:10.5061/dryad.kn45n49/4 doi:10.5061/dryad.kn45n49/5 doi:10.5061/dryad.kn45n49/6 doi:10.5061/dryad.kn45n49/7 doi:10.5061/dryad.kn45n49/8 doi:10.5061/dryad.kn45n49/9 doi:10.5061/dryad.kn45n49/10 doi:10.5061/dryad.kn45n49/11 doi:10.5061/dryad.kn45n49/12 10.1098/rspb http://nbn-resolving.org/urn:nbn:nl:ui:13-ia-2mcn doi:10.5061/dryad.kn45n49 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:113704 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf |
op_doi |
https://doi.org/10.5061/dryad.kn45n49/110.5061/dryad.kn45n49/210.5061/dryad.kn45n49/310.5061/dryad.kn45n49/410.5061/dryad.kn45n49/510.5061/dryad.kn45n49/610.5061/dryad.kn45n49/710.5061/dryad.kn45n49/810.5061/dryad.kn45n49/910.5061/dryad.kn45n49/1010.5061/ |
container_title |
Journal of Oleo Science |
container_volume |
59 |
container_issue |
6 |
container_start_page |
281 |
op_container_end_page |
292 |
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1770271518376329216 |