Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus)
The management of hunted species is challenging, as it must conciliate the conservation of species and their sustainable exploitation. Non-genetic tools are widely used in this context but they may present limitations notably when species can hybridize or when large-scale spatial monitoring is requi...
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ftdans:oai:easy.dans.knaw.nl:easy-dataset:102152 2023-07-02T03:32:53+02:00 Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) Beugin, Marie-Pauline Letty, Jérôme Kaerle, Cécile Guitton, Jean-Sébastien Muselet, Lina Queney, Guillaume Pontier, Dominique 2017-04-25T17:09:27.000+02:00 http://nbn-resolving.org/urn:nbn:nl:ui:13-gd-j0h2 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:102152 unknown doi:10.5061/dryad.d4368/1 doi:10.1002/ece3.2943 http://nbn-resolving.org/urn:nbn:nl:ui:13-gd-j0h2 doi:10.5061/dryad.d4368 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:102152 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Life sciences medicine and health care 2017 ftdans https://doi.org/10.5061/dryad.d4368/110.1002/ece3.294310.5061/dryad.d4368 2023-06-13T13:27:52Z The management of hunted species is challenging, as it must conciliate the conservation of species and their sustainable exploitation. Non-genetic tools are widely used in this context but they may present limitations notably when species can hybridize or when large-scale spatial monitoring is required to establish optimal management actions. This is why genetic tools have been more and more integrated in wildlife management practices. However, the markers proposed are often amplified in small multiplexes when larger ones could allow to better cope with the small quantities of DNA obtained with non-invasive sampling methods. Here, we propose a unique multiplex of 12 autosomal microsatellite markers for the study of two hare species that exist in sympatry in some areas in Europe and are hunted notably in France: the brown hare Lepus europaeus and the mountain hare L. timidus. We tested 17 markers previously used in these two species or other lagomorph species, from which 12 were included in this single multiplex. Diversity was between 4 and 30 alleles per locus totalling 126 alleles and we showed that these markers possess appropriate genetic resolution for individual and species identification for the populations under study. This multiplex panel represents the largest number of microsatellites amplified in one reaction proposed for these two hare species and provides a cost-effective and valuable tool for further hybridization studies and the management of hares. Other/Unknown Material Lepus timidus mountain hare Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
institution |
Open Polar |
collection |
Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
op_collection_id |
ftdans |
language |
unknown |
topic |
Life sciences medicine and health care |
spellingShingle |
Life sciences medicine and health care Beugin, Marie-Pauline Letty, Jérôme Kaerle, Cécile Guitton, Jean-Sébastien Muselet, Lina Queney, Guillaume Pontier, Dominique Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
topic_facet |
Life sciences medicine and health care |
description |
The management of hunted species is challenging, as it must conciliate the conservation of species and their sustainable exploitation. Non-genetic tools are widely used in this context but they may present limitations notably when species can hybridize or when large-scale spatial monitoring is required to establish optimal management actions. This is why genetic tools have been more and more integrated in wildlife management practices. However, the markers proposed are often amplified in small multiplexes when larger ones could allow to better cope with the small quantities of DNA obtained with non-invasive sampling methods. Here, we propose a unique multiplex of 12 autosomal microsatellite markers for the study of two hare species that exist in sympatry in some areas in Europe and are hunted notably in France: the brown hare Lepus europaeus and the mountain hare L. timidus. We tested 17 markers previously used in these two species or other lagomorph species, from which 12 were included in this single multiplex. Diversity was between 4 and 30 alleles per locus totalling 126 alleles and we showed that these markers possess appropriate genetic resolution for individual and species identification for the populations under study. This multiplex panel represents the largest number of microsatellites amplified in one reaction proposed for these two hare species and provides a cost-effective and valuable tool for further hybridization studies and the management of hares. |
author |
Beugin, Marie-Pauline Letty, Jérôme Kaerle, Cécile Guitton, Jean-Sébastien Muselet, Lina Queney, Guillaume Pontier, Dominique |
author_facet |
Beugin, Marie-Pauline Letty, Jérôme Kaerle, Cécile Guitton, Jean-Sébastien Muselet, Lina Queney, Guillaume Pontier, Dominique |
author_sort |
Beugin, Marie-Pauline |
title |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_short |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_full |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_fullStr |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_full_unstemmed |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_sort |
data from: a single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (lepus europaeus) and the mountain hare (lepus timidus) |
publishDate |
2017 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-gd-j0h2 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:102152 |
genre |
Lepus timidus mountain hare |
genre_facet |
Lepus timidus mountain hare |
op_relation |
doi:10.5061/dryad.d4368/1 doi:10.1002/ece3.2943 http://nbn-resolving.org/urn:nbn:nl:ui:13-gd-j0h2 doi:10.5061/dryad.d4368 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:102152 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf |
op_doi |
https://doi.org/10.5061/dryad.d4368/110.1002/ece3.294310.5061/dryad.d4368 |
_version_ |
1770272588398854144 |