THE MOLECULAR CONSEQUENCES OF VITAMIN B12 DEPRIVATION IN THE POLAR DIATOM, FRAGILARIOPSIS CYLINDRUS
Fragilariopsis cylindrus is an ecologically significant diatom inhabiting sea ice and water column assemblages in the Southern Ocean (SO). Microbial growth in SO microbial communities has been demonstrated to be periodically limited or co-limited by vitamin B12. In this dissertation, I examine the m...
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ftdalhouse:oai:DalSpace.library.dal.ca:10222/81567 2023-05-15T18:18:19+02:00 THE MOLECULAR CONSEQUENCES OF VITAMIN B12 DEPRIVATION IN THE POLAR DIATOM, FRAGILARIOPSIS CYLINDRUS Albury, Catalina Department of Biology Master of Science na Dr. Daniel Ruzzante Dr. Zoe Finkel Dr. John Archibald Dr. Erin Bertrand Not Applicable 2022-04-14T14:58:14Z http://hdl.handle.net/10222/81567 en eng http://hdl.handle.net/10222/81567 vitamin B12 Fragilariopsis cylindrus proteomics mass spectrometry metabolites 2022 ftdalhouse 2022-07-30T23:10:36Z Fragilariopsis cylindrus is an ecologically significant diatom inhabiting sea ice and water column assemblages in the Southern Ocean (SO). Microbial growth in SO microbial communities has been demonstrated to be periodically limited or co-limited by vitamin B12. In this dissertation, I examine the molecular consequences of B12 deprivation in F. cylindrus, with a focus on alterations to protein expression and metabolite production. Despite unchanged growth rates, B12-deprived F. cylindrus cells display responses that can impact the production of growth stimulating compounds, including induction of a putative B12 remodeling pathway and differential production of DMSP and thiamine related compounds. My findings highlight that assessments of marine phytoplankton’s responses to stressors beyond growth rate should be considered in order to generate accurate predictions of community interactions in micronutrient deprived environments. These results have implications for our understanding the relationship between B12 availability and community composition and nutrient cycling in a changing ocean. Other/Unknown Material Sea ice Southern Ocean Dalhousie University: DalSpace Institutional Repository Southern Ocean |
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Open Polar |
collection |
Dalhousie University: DalSpace Institutional Repository |
op_collection_id |
ftdalhouse |
language |
English |
topic |
vitamin B12 Fragilariopsis cylindrus proteomics mass spectrometry metabolites |
spellingShingle |
vitamin B12 Fragilariopsis cylindrus proteomics mass spectrometry metabolites Albury, Catalina THE MOLECULAR CONSEQUENCES OF VITAMIN B12 DEPRIVATION IN THE POLAR DIATOM, FRAGILARIOPSIS CYLINDRUS |
topic_facet |
vitamin B12 Fragilariopsis cylindrus proteomics mass spectrometry metabolites |
description |
Fragilariopsis cylindrus is an ecologically significant diatom inhabiting sea ice and water column assemblages in the Southern Ocean (SO). Microbial growth in SO microbial communities has been demonstrated to be periodically limited or co-limited by vitamin B12. In this dissertation, I examine the molecular consequences of B12 deprivation in F. cylindrus, with a focus on alterations to protein expression and metabolite production. Despite unchanged growth rates, B12-deprived F. cylindrus cells display responses that can impact the production of growth stimulating compounds, including induction of a putative B12 remodeling pathway and differential production of DMSP and thiamine related compounds. My findings highlight that assessments of marine phytoplankton’s responses to stressors beyond growth rate should be considered in order to generate accurate predictions of community interactions in micronutrient deprived environments. These results have implications for our understanding the relationship between B12 availability and community composition and nutrient cycling in a changing ocean. |
author2 |
Department of Biology Master of Science na Dr. Daniel Ruzzante Dr. Zoe Finkel Dr. John Archibald Dr. Erin Bertrand Not Applicable |
author |
Albury, Catalina |
author_facet |
Albury, Catalina |
author_sort |
Albury, Catalina |
title |
THE MOLECULAR CONSEQUENCES OF VITAMIN B12 DEPRIVATION IN THE POLAR DIATOM, FRAGILARIOPSIS CYLINDRUS |
title_short |
THE MOLECULAR CONSEQUENCES OF VITAMIN B12 DEPRIVATION IN THE POLAR DIATOM, FRAGILARIOPSIS CYLINDRUS |
title_full |
THE MOLECULAR CONSEQUENCES OF VITAMIN B12 DEPRIVATION IN THE POLAR DIATOM, FRAGILARIOPSIS CYLINDRUS |
title_fullStr |
THE MOLECULAR CONSEQUENCES OF VITAMIN B12 DEPRIVATION IN THE POLAR DIATOM, FRAGILARIOPSIS CYLINDRUS |
title_full_unstemmed |
THE MOLECULAR CONSEQUENCES OF VITAMIN B12 DEPRIVATION IN THE POLAR DIATOM, FRAGILARIOPSIS CYLINDRUS |
title_sort |
molecular consequences of vitamin b12 deprivation in the polar diatom, fragilariopsis cylindrus |
publishDate |
2022 |
url |
http://hdl.handle.net/10222/81567 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Sea ice Southern Ocean |
genre_facet |
Sea ice Southern Ocean |
op_relation |
http://hdl.handle.net/10222/81567 |
_version_ |
1766194858138009600 |