Identification and Aspects of Commercial Production of Anti-Diabetic Peptide(s) from Salmon Protein Hydrolysates
A low molecular weight (< 1 kDa) hydrolysate of Atlantic salmon protein has previously been shown to have anti-diabetic effects in vitro and in mouse models. This salmon peptide fraction (SPF) produced using the enzymes pepsin, trypsin and chymotrypsin, contains hundreds of potential bioactive co...
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ftdalhouse:oai:DalSpace.library.dal.ca:10222/80024 2023-05-15T15:31:39+02:00 Identification and Aspects of Commercial Production of Anti-Diabetic Peptide(s) from Salmon Protein Hydrolysates Rolin, Jonathan Department of Process Engineering and Applied Science Doctor of Philosophy Dr. David Kitts Dr. Suzanne Budge Dr. Laurent Bazinet Dr. Andre Marette Dr. Tom Gill Dr. Allan Paulson Not Applicable 2020-11-23T12:28:35Z http://hdl.handle.net/10222/80024 en eng http://hdl.handle.net/10222/80024 Bioactive Peptides Atlantic Salmon Type 2 Diabetes Thesis 2020 ftdalhouse 2022-03-06T00:10:52Z A low molecular weight (< 1 kDa) hydrolysate of Atlantic salmon protein has previously been shown to have anti-diabetic effects in vitro and in mouse models. This salmon peptide fraction (SPF) produced using the enzymes pepsin, trypsin and chymotrypsin, contains hundreds of potential bioactive compounds and the identification the functional modulators of this activity may realize novel therapeutic compounds or targets as treatments for type 2 diabetes. The aim of this study was therefore to identify the sequences and characteristics of potential bioactive peptides from the SPF with a functional effect in cultured L6 myotubes. Separation of progenitor proteins by electrophoresis and of SPF by column chromatography with gel filtration and strong anion exchange formats were successful at concentrating bioactive peptides into multiple subfractions. Tandem mass spectrometry of subfractions suggested that di- and tripeptides composed of Ile/Leu, Val, Asp, Glu, Trp, Tyr were common among peptides identified in bioactive fractions. The importance of functional peptide concentration and sequence motifs on bioactivity was tested using the synthetic sequences Ile-Ala-Tyr and Ile-Gly-Tyr and exhibited a stimulating effect at 2.8 nM, but an inhibiting effect at 2.8 pM. Swiss Target Prediction suggested these sequences are peptidomimetics of agonists to mu-type, delta-type and kappa-type opioid receptors. When considering the large total number of peptides and abundance of sequences containing similar motifs in SPF, its bioactivity is likely the result of complex interactions of many peptides. Thesis Atlantic salmon Dalhousie University: DalSpace Institutional Repository |
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Dalhousie University: DalSpace Institutional Repository |
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language |
English |
topic |
Bioactive Peptides Atlantic Salmon Type 2 Diabetes |
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Bioactive Peptides Atlantic Salmon Type 2 Diabetes Rolin, Jonathan Identification and Aspects of Commercial Production of Anti-Diabetic Peptide(s) from Salmon Protein Hydrolysates |
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Bioactive Peptides Atlantic Salmon Type 2 Diabetes |
description |
A low molecular weight (< 1 kDa) hydrolysate of Atlantic salmon protein has previously been shown to have anti-diabetic effects in vitro and in mouse models. This salmon peptide fraction (SPF) produced using the enzymes pepsin, trypsin and chymotrypsin, contains hundreds of potential bioactive compounds and the identification the functional modulators of this activity may realize novel therapeutic compounds or targets as treatments for type 2 diabetes. The aim of this study was therefore to identify the sequences and characteristics of potential bioactive peptides from the SPF with a functional effect in cultured L6 myotubes. Separation of progenitor proteins by electrophoresis and of SPF by column chromatography with gel filtration and strong anion exchange formats were successful at concentrating bioactive peptides into multiple subfractions. Tandem mass spectrometry of subfractions suggested that di- and tripeptides composed of Ile/Leu, Val, Asp, Glu, Trp, Tyr were common among peptides identified in bioactive fractions. The importance of functional peptide concentration and sequence motifs on bioactivity was tested using the synthetic sequences Ile-Ala-Tyr and Ile-Gly-Tyr and exhibited a stimulating effect at 2.8 nM, but an inhibiting effect at 2.8 pM. Swiss Target Prediction suggested these sequences are peptidomimetics of agonists to mu-type, delta-type and kappa-type opioid receptors. When considering the large total number of peptides and abundance of sequences containing similar motifs in SPF, its bioactivity is likely the result of complex interactions of many peptides. |
author2 |
Department of Process Engineering and Applied Science Doctor of Philosophy Dr. David Kitts Dr. Suzanne Budge Dr. Laurent Bazinet Dr. Andre Marette Dr. Tom Gill Dr. Allan Paulson Not Applicable |
format |
Thesis |
author |
Rolin, Jonathan |
author_facet |
Rolin, Jonathan |
author_sort |
Rolin, Jonathan |
title |
Identification and Aspects of Commercial Production of Anti-Diabetic Peptide(s) from Salmon Protein Hydrolysates |
title_short |
Identification and Aspects of Commercial Production of Anti-Diabetic Peptide(s) from Salmon Protein Hydrolysates |
title_full |
Identification and Aspects of Commercial Production of Anti-Diabetic Peptide(s) from Salmon Protein Hydrolysates |
title_fullStr |
Identification and Aspects of Commercial Production of Anti-Diabetic Peptide(s) from Salmon Protein Hydrolysates |
title_full_unstemmed |
Identification and Aspects of Commercial Production of Anti-Diabetic Peptide(s) from Salmon Protein Hydrolysates |
title_sort |
identification and aspects of commercial production of anti-diabetic peptide(s) from salmon protein hydrolysates |
publishDate |
2020 |
url |
http://hdl.handle.net/10222/80024 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://hdl.handle.net/10222/80024 |
_version_ |
1766362181117411328 |