Separation and purification of antidiabetic bioactive peptide from salmon and cod waste
Dietary proteins from Atlantic salmon and cod have previously been shown to have antidiabetic effects. Since dietary proteins are digested into small peptides before being absorbed through the intestinal mucosa, it is reasonable to deduce that the antidiabetic effect is due to enzymatically-digested...
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ftdalhouse:oai:DalSpace.library.dal.ca:10222/37804 2024-06-02T08:03:39+00:00 Separation and purification of antidiabetic bioactive peptide from salmon and cod waste Jin, Tianyi Jr Department of Process Engineering and Applied Science Master of Science Roger S. McLeod Georges J. Kipouros Allan A. Paulson Tom A. Gill Not Applicable 2013-10-28T16:22:19Z http://hdl.handle.net/10222/37804 en eng http://hdl.handle.net/10222/37804 Type 2 diabetes salmon and cod waste antidiabetic peptides 2013 ftdalhouse 2024-05-06T11:40:25Z Dietary proteins from Atlantic salmon and cod have previously been shown to have antidiabetic effects. Since dietary proteins are digested into small peptides before being absorbed through the intestinal mucosa, it is reasonable to deduce that the antidiabetic effect is due to enzymatically-digested peptides rather than the proteins themselves. The aim of this study was to develop a protocol to recover peptides with antidiabetic effects from salmon and cod protein digests and then scale up and optimize the salmon protein hydrolysate production process for industrial-scale production. The peptide mixtures were screened using cell culture assays for insulin-modulating activities and were further fractionated and purified for the final identification. Total yields of salmon and cod protein hydrolysates (<1 kDa) as measured by Kjeldahl nitrogen were 16.9% and 40.1%, respectively. The production process used for the salmon protein hydrolysate (<1 kDa) showed good reproducibility and potential for the industrial-scale production. Other/Unknown Material Atlantic salmon Dalhousie University: DalSpace Institutional Repository |
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Dalhousie University: DalSpace Institutional Repository |
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language |
English |
topic |
Type 2 diabetes salmon and cod waste antidiabetic peptides |
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Type 2 diabetes salmon and cod waste antidiabetic peptides Jin, Tianyi Jr Separation and purification of antidiabetic bioactive peptide from salmon and cod waste |
topic_facet |
Type 2 diabetes salmon and cod waste antidiabetic peptides |
description |
Dietary proteins from Atlantic salmon and cod have previously been shown to have antidiabetic effects. Since dietary proteins are digested into small peptides before being absorbed through the intestinal mucosa, it is reasonable to deduce that the antidiabetic effect is due to enzymatically-digested peptides rather than the proteins themselves. The aim of this study was to develop a protocol to recover peptides with antidiabetic effects from salmon and cod protein digests and then scale up and optimize the salmon protein hydrolysate production process for industrial-scale production. The peptide mixtures were screened using cell culture assays for insulin-modulating activities and were further fractionated and purified for the final identification. Total yields of salmon and cod protein hydrolysates (<1 kDa) as measured by Kjeldahl nitrogen were 16.9% and 40.1%, respectively. The production process used for the salmon protein hydrolysate (<1 kDa) showed good reproducibility and potential for the industrial-scale production. |
author2 |
Department of Process Engineering and Applied Science Master of Science Roger S. McLeod Georges J. Kipouros Allan A. Paulson Tom A. Gill Not Applicable |
author |
Jin, Tianyi Jr |
author_facet |
Jin, Tianyi Jr |
author_sort |
Jin, Tianyi Jr |
title |
Separation and purification of antidiabetic bioactive peptide from salmon and cod waste |
title_short |
Separation and purification of antidiabetic bioactive peptide from salmon and cod waste |
title_full |
Separation and purification of antidiabetic bioactive peptide from salmon and cod waste |
title_fullStr |
Separation and purification of antidiabetic bioactive peptide from salmon and cod waste |
title_full_unstemmed |
Separation and purification of antidiabetic bioactive peptide from salmon and cod waste |
title_sort |
separation and purification of antidiabetic bioactive peptide from salmon and cod waste |
publishDate |
2013 |
url |
http://hdl.handle.net/10222/37804 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://hdl.handle.net/10222/37804 |
_version_ |
1800748243074678784 |