Three-dimensional reconstruction of the S885A mutant of human mitochondrial Lon protease
The Lon protein is a protease belonging to the superfamily of ATPases associated with diverse cellular activities (AAA+). Its main function is the control of protein quality and the maintenance of proteostasis by degradation of misfolded and damaged proteins, which occur in response to numerous stre...
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ftczechacademysc:oai:kramerius.lib.cas.cz:uuid:a772303b-908a-456b-9373-7beb411040e4 2024-03-17T09:00:04+00:00 Three-dimensional reconstruction of the S885A mutant of human mitochondrial Lon protease Kereïche, S. Kováčik, L. Pevala, V. Ambro, L. Bellová, J. Kutejová, L. Raška, I. [62]-65 média svazek https://kramerius.lib.cas.cz/view/uuid:a772303b-908a-456b-9373-7beb411040e4 unknown https://kramerius.lib.cas.cz/view/uuid:a772303b-908a-456b-9373-7beb411040e4 policy:private transmission electron microscopy 3D reconstruction AAA+ protease human mitochondrial protein model:article ftczechacademysc 2024-02-19T23:32:25Z The Lon protein is a protease belonging to the superfamily of ATPases associated with diverse cellular activities (AAA+). Its main function is the control of protein quality and the maintenance of proteostasis by degradation of misfolded and damaged proteins, which occur in response to numerous stress conditions. It also participates in the regulation of levels of transcription factors that control pathogenesis, development and stress response. We focus our interest on the structure of human mitochondrial Lon (hLon) protease, whose altered expression levels are linked to some severe diseases such as epilepsy, myopathy, or lateral sclerosis. We present the first 3D structure of the ADP-bound human Lon S885A mutant obtained by electron microscopy as a result of preliminary negative staining studies. S885A appears as a hexameric ring of 120 Å diameter having 90 Å in height. Its resolution was estimated at 19 Å by the FSC = 0.5 criterion. This model is a primary step towards the understanding of the mechanism of action of the Lon protease and its involvement in the pathogenesis development Corresponding author: Sami Kereïche Article in Journal/Newspaper sami Czech Academy of Sciences: dKNAV |
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Open Polar |
collection |
Czech Academy of Sciences: dKNAV |
op_collection_id |
ftczechacademysc |
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unknown |
topic |
transmission electron microscopy 3D reconstruction AAA+ protease human mitochondrial protein |
spellingShingle |
transmission electron microscopy 3D reconstruction AAA+ protease human mitochondrial protein Kereïche, S. Kováčik, L. Pevala, V. Ambro, L. Bellová, J. Kutejová, L. Raška, I. Three-dimensional reconstruction of the S885A mutant of human mitochondrial Lon protease |
topic_facet |
transmission electron microscopy 3D reconstruction AAA+ protease human mitochondrial protein |
description |
The Lon protein is a protease belonging to the superfamily of ATPases associated with diverse cellular activities (AAA+). Its main function is the control of protein quality and the maintenance of proteostasis by degradation of misfolded and damaged proteins, which occur in response to numerous stress conditions. It also participates in the regulation of levels of transcription factors that control pathogenesis, development and stress response. We focus our interest on the structure of human mitochondrial Lon (hLon) protease, whose altered expression levels are linked to some severe diseases such as epilepsy, myopathy, or lateral sclerosis. We present the first 3D structure of the ADP-bound human Lon S885A mutant obtained by electron microscopy as a result of preliminary negative staining studies. S885A appears as a hexameric ring of 120 Å diameter having 90 Å in height. Its resolution was estimated at 19 Å by the FSC = 0.5 criterion. This model is a primary step towards the understanding of the mechanism of action of the Lon protease and its involvement in the pathogenesis development Corresponding author: Sami Kereïche |
format |
Article in Journal/Newspaper |
author |
Kereïche, S. Kováčik, L. Pevala, V. Ambro, L. Bellová, J. Kutejová, L. Raška, I. |
author_facet |
Kereïche, S. Kováčik, L. Pevala, V. Ambro, L. Bellová, J. Kutejová, L. Raška, I. |
author_sort |
Kereïche, S. |
title |
Three-dimensional reconstruction of the S885A mutant of human mitochondrial Lon protease |
title_short |
Three-dimensional reconstruction of the S885A mutant of human mitochondrial Lon protease |
title_full |
Three-dimensional reconstruction of the S885A mutant of human mitochondrial Lon protease |
title_fullStr |
Three-dimensional reconstruction of the S885A mutant of human mitochondrial Lon protease |
title_full_unstemmed |
Three-dimensional reconstruction of the S885A mutant of human mitochondrial Lon protease |
title_sort |
three-dimensional reconstruction of the s885a mutant of human mitochondrial lon protease |
url |
https://kramerius.lib.cas.cz/view/uuid:a772303b-908a-456b-9373-7beb411040e4 |
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[62]-65 |
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sami |
op_relation |
https://kramerius.lib.cas.cz/view/uuid:a772303b-908a-456b-9373-7beb411040e4 |
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policy:private |
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1793771225895403520 |