Sensitivity of photosystem 2 of Antarctic lichens to high irradiance stress: Fluorometric study of fruticose (Usnea antarctica) and foliose (Umbilicaria decussata) species
Two lichen species collected in maritime Antarctica (King George Island) were exposed under laboratory conditions to excess irradiance to evaluate the response of photosystem 2 (PS2). The response was measured on fully hydrated lichen thalli at 5 °C by means of a modulated fluorometer using chloroph...
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ftczechacademysc:oai:kramerius.lib.cas.cz:uuid:41558ab5-638a-43cd-94ee-e184dffebd57 2024-03-17T08:53:38+00:00 Sensitivity of photosystem 2 of Antarctic lichens to high irradiance stress: Fluorometric study of fruticose (Usnea antarctica) and foliose (Umbilicaria decussata) species Barták, M. Vráblíková, H. Hájek, J. 497-504 média svazek https://kramerius.lib.cas.cz/view/uuid:41558ab5-638a-43cd-94ee-e184dffebd57 https://doi.org/10.1023/B:PHOT.0000027513.90599.ad eng eng https://kramerius.lib.cas.cz/view/uuid:41558ab5-638a-43cd-94ee-e184dffebd57 issn:0300-3604 doi:10.1023/B:PHOT.0000027513.90599.ad policy:public chlorophyll fluorescence low temperature photoinhibition photosynthesis non-photochemical quenching ftczechacademysc https://doi.org/10.1023/B:PHOT.0000027513.90599.ad 2024-02-19T23:10:29Z Two lichen species collected in maritime Antarctica (King George Island) were exposed under laboratory conditions to excess irradiance to evaluate the response of photosystem 2 (PS2). The response was measured on fully hydrated lichen thalli at 5 °C by means of a modulated fluorometer using chlorophyll (Chl) fluorescence induction curve supplemented with analysis of quenching mechanisms. Chl fluorescence parameters [i.e. ratio of variable to maximum Chl fluorescence (FV/FM), quantum yield of PS2 photochemical reactions (Φ2), quenching coefficients] were evaluated before and several times after exposition to high irradiance in order to characterise the extent of photoinhibition, fast and slow phase of recovery. Strong irradiance (2 000 μmol m-2 s-1) caused high degree of photoinhibition, particularly higher in fruticose (Usnea antarctica) than in foliose (Umbilicaria decussata) lichen species. Fast phase of recovery from photoinhibition, corresponding to regulatory mechanisms of PS2, was more apparent in U. decussata and Φ2 than in U. antarctica and FV/FM and Φ2 within 40 min after photoinhibitory treatment. It was followed by a slow phase lasting several hours, corresponding to repair and re-synthesis processes. After photoinhibitory treatment, recovery of non-photochemical quenching (NPQ) was faster and more pronounced in U. decussata than in U. antarctica. Significant differences were found between the two species in the rate of recovery in fast-(qE) and slow-recovering (qT+I) component of NPQ. M. Barták, H. Vráblíková, J. Hájek. Other/Unknown Material Antarc* Antarctic Antarctica King George Island Usnea antarctica Czech Academy of Sciences: dKNAV Antarctic King George Island |
institution |
Open Polar |
collection |
Czech Academy of Sciences: dKNAV |
op_collection_id |
ftczechacademysc |
language |
English |
topic |
chlorophyll fluorescence low temperature photoinhibition photosynthesis non-photochemical quenching |
spellingShingle |
chlorophyll fluorescence low temperature photoinhibition photosynthesis non-photochemical quenching Barták, M. Vráblíková, H. Hájek, J. Sensitivity of photosystem 2 of Antarctic lichens to high irradiance stress: Fluorometric study of fruticose (Usnea antarctica) and foliose (Umbilicaria decussata) species |
topic_facet |
chlorophyll fluorescence low temperature photoinhibition photosynthesis non-photochemical quenching |
description |
Two lichen species collected in maritime Antarctica (King George Island) were exposed under laboratory conditions to excess irradiance to evaluate the response of photosystem 2 (PS2). The response was measured on fully hydrated lichen thalli at 5 °C by means of a modulated fluorometer using chlorophyll (Chl) fluorescence induction curve supplemented with analysis of quenching mechanisms. Chl fluorescence parameters [i.e. ratio of variable to maximum Chl fluorescence (FV/FM), quantum yield of PS2 photochemical reactions (Φ2), quenching coefficients] were evaluated before and several times after exposition to high irradiance in order to characterise the extent of photoinhibition, fast and slow phase of recovery. Strong irradiance (2 000 μmol m-2 s-1) caused high degree of photoinhibition, particularly higher in fruticose (Usnea antarctica) than in foliose (Umbilicaria decussata) lichen species. Fast phase of recovery from photoinhibition, corresponding to regulatory mechanisms of PS2, was more apparent in U. decussata and Φ2 than in U. antarctica and FV/FM and Φ2 within 40 min after photoinhibitory treatment. It was followed by a slow phase lasting several hours, corresponding to repair and re-synthesis processes. After photoinhibitory treatment, recovery of non-photochemical quenching (NPQ) was faster and more pronounced in U. decussata than in U. antarctica. Significant differences were found between the two species in the rate of recovery in fast-(qE) and slow-recovering (qT+I) component of NPQ. M. Barták, H. Vráblíková, J. Hájek. |
author |
Barták, M. Vráblíková, H. Hájek, J. |
author_facet |
Barták, M. Vráblíková, H. Hájek, J. |
author_sort |
Barták, M. |
title |
Sensitivity of photosystem 2 of Antarctic lichens to high irradiance stress: Fluorometric study of fruticose (Usnea antarctica) and foliose (Umbilicaria decussata) species |
title_short |
Sensitivity of photosystem 2 of Antarctic lichens to high irradiance stress: Fluorometric study of fruticose (Usnea antarctica) and foliose (Umbilicaria decussata) species |
title_full |
Sensitivity of photosystem 2 of Antarctic lichens to high irradiance stress: Fluorometric study of fruticose (Usnea antarctica) and foliose (Umbilicaria decussata) species |
title_fullStr |
Sensitivity of photosystem 2 of Antarctic lichens to high irradiance stress: Fluorometric study of fruticose (Usnea antarctica) and foliose (Umbilicaria decussata) species |
title_full_unstemmed |
Sensitivity of photosystem 2 of Antarctic lichens to high irradiance stress: Fluorometric study of fruticose (Usnea antarctica) and foliose (Umbilicaria decussata) species |
title_sort |
sensitivity of photosystem 2 of antarctic lichens to high irradiance stress: fluorometric study of fruticose (usnea antarctica) and foliose (umbilicaria decussata) species |
url |
https://kramerius.lib.cas.cz/view/uuid:41558ab5-638a-43cd-94ee-e184dffebd57 https://doi.org/10.1023/B:PHOT.0000027513.90599.ad |
op_coverage |
497-504 |
geographic |
Antarctic King George Island |
geographic_facet |
Antarctic King George Island |
genre |
Antarc* Antarctic Antarctica King George Island Usnea antarctica |
genre_facet |
Antarc* Antarctic Antarctica King George Island Usnea antarctica |
op_relation |
https://kramerius.lib.cas.cz/view/uuid:41558ab5-638a-43cd-94ee-e184dffebd57 issn:0300-3604 doi:10.1023/B:PHOT.0000027513.90599.ad |
op_rights |
policy:public |
op_doi |
https://doi.org/10.1023/B:PHOT.0000027513.90599.ad |
_version_ |
1793766933503410176 |