Cryoprotectant systems and cold tolerance of insects inhabiting central Yakutia (Russian Far East)
Strong tolerance of freezing is an important strategy for insects living in extremely cold regions. They produce highly effective cryoprotectant systems consisting of ice-nucleating proteins and polyols, which enables tolerable freezing of the body fluid. Therefore, the measurement of the concentrat...
Published in: | European Journal of Entomology |
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ftczechacademysc:oai:kramerius.lib.cas.cz:uuid:32e97e07-f01d-4138-a973-6db3ac48c7dd 2024-03-17T09:00:34+00:00 Cryoprotectant systems and cold tolerance of insects inhabiting central Yakutia (Russian Far East) Li, Natalia G. print počítač zdroj https://kramerius.lib.cas.cz/view/uuid:32e97e07-f01d-4138-a973-6db3ac48c7dd https://doi.org/10.14411/eje.2016.073 unknown https://kramerius.lib.cas.cz/view/uuid:32e97e07-f01d-4138-a973-6db3ac48c7dd issn:1210-5759 doi:10.14411/eje.2016.073 policy:public Zoologie glycerol hmyz insects freeze-tolerance ice nucleators cold hardiness potential Siberian insects 2 59 article články journal articles model:article ftczechacademysc https://doi.org/10.14411/eje.2016.073 2024-02-19T23:03:31Z Strong tolerance of freezing is an important strategy for insects living in extremely cold regions. They produce highly effective cryoprotectant systems consisting of ice-nucleating proteins and polyols, which enables tolerable freezing of the body fluid. Therefore, the measurement of the concentrations of polyols and the activity of ice nucleators in the haemolymph is an essential tool for describing tolerance to ice formation in insects occurring in particularly cold places. This study evaluates three parameters: insect body supercooling point (SCP), haemolymph glycerol content and the profile of haemolymph ice nucleating activity that characterize the strategies of cold adaptation and cold hardiness in two previously unstudied beetles, Chrysolina graminis graminis L. and Galerucella nymphaea L., inhabiting Yakutia (Russian Far East, latitude 62°N). The high SCP values, ice nucleating activity and survival of the chrysomelids after freezing indicate that both species are tolerant of freezing. According to the profiles of ice-nucleating activity, the haemolymph from C. graminis graminis is characterized by a higher nucleating potential than that from G. nymphaea. The glycerol level is also higher in C. graminis graminis. The results indicate that both species develop tolerance to low temperatures, but the cold hardiness potential of C. graminis graminis is greater than that of G. nymphaea. This was revealed by the survival test, in which beetles were frozen to a temperature of -22°C for 30 min; 86% of C. graminis graminis and 72% of G. nymphaea survived the test. Thus, the freeze-tolerance of these beetles seems to be based on the production of an integrated cryoprotectant system, the quality of which apparently influences the range of their cold resistance. Natalia G. Li. Obsahuje bibliografii Article in Journal/Newspaper Yakutia Czech Academy of Sciences: dKNAV European Journal of Entomology 113 537 541 |
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Open Polar |
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Czech Academy of Sciences: dKNAV |
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ftczechacademysc |
language |
unknown |
topic |
Zoologie glycerol hmyz insects freeze-tolerance ice nucleators cold hardiness potential Siberian insects 2 59 |
spellingShingle |
Zoologie glycerol hmyz insects freeze-tolerance ice nucleators cold hardiness potential Siberian insects 2 59 Li, Natalia G. Cryoprotectant systems and cold tolerance of insects inhabiting central Yakutia (Russian Far East) |
topic_facet |
Zoologie glycerol hmyz insects freeze-tolerance ice nucleators cold hardiness potential Siberian insects 2 59 |
description |
Strong tolerance of freezing is an important strategy for insects living in extremely cold regions. They produce highly effective cryoprotectant systems consisting of ice-nucleating proteins and polyols, which enables tolerable freezing of the body fluid. Therefore, the measurement of the concentrations of polyols and the activity of ice nucleators in the haemolymph is an essential tool for describing tolerance to ice formation in insects occurring in particularly cold places. This study evaluates three parameters: insect body supercooling point (SCP), haemolymph glycerol content and the profile of haemolymph ice nucleating activity that characterize the strategies of cold adaptation and cold hardiness in two previously unstudied beetles, Chrysolina graminis graminis L. and Galerucella nymphaea L., inhabiting Yakutia (Russian Far East, latitude 62°N). The high SCP values, ice nucleating activity and survival of the chrysomelids after freezing indicate that both species are tolerant of freezing. According to the profiles of ice-nucleating activity, the haemolymph from C. graminis graminis is characterized by a higher nucleating potential than that from G. nymphaea. The glycerol level is also higher in C. graminis graminis. The results indicate that both species develop tolerance to low temperatures, but the cold hardiness potential of C. graminis graminis is greater than that of G. nymphaea. This was revealed by the survival test, in which beetles were frozen to a temperature of -22°C for 30 min; 86% of C. graminis graminis and 72% of G. nymphaea survived the test. Thus, the freeze-tolerance of these beetles seems to be based on the production of an integrated cryoprotectant system, the quality of which apparently influences the range of their cold resistance. Natalia G. Li. Obsahuje bibliografii |
format |
Article in Journal/Newspaper |
author |
Li, Natalia G. |
author_facet |
Li, Natalia G. |
author_sort |
Li, Natalia G. |
title |
Cryoprotectant systems and cold tolerance of insects inhabiting central Yakutia (Russian Far East) |
title_short |
Cryoprotectant systems and cold tolerance of insects inhabiting central Yakutia (Russian Far East) |
title_full |
Cryoprotectant systems and cold tolerance of insects inhabiting central Yakutia (Russian Far East) |
title_fullStr |
Cryoprotectant systems and cold tolerance of insects inhabiting central Yakutia (Russian Far East) |
title_full_unstemmed |
Cryoprotectant systems and cold tolerance of insects inhabiting central Yakutia (Russian Far East) |
title_sort |
cryoprotectant systems and cold tolerance of insects inhabiting central yakutia (russian far east) |
url |
https://kramerius.lib.cas.cz/view/uuid:32e97e07-f01d-4138-a973-6db3ac48c7dd https://doi.org/10.14411/eje.2016.073 |
genre |
Yakutia |
genre_facet |
Yakutia |
op_relation |
https://kramerius.lib.cas.cz/view/uuid:32e97e07-f01d-4138-a973-6db3ac48c7dd issn:1210-5759 doi:10.14411/eje.2016.073 |
op_rights |
policy:public |
op_doi |
https://doi.org/10.14411/eje.2016.073 |
container_title |
European Journal of Entomology |
container_volume |
113 |
container_start_page |
537 |
op_container_end_page |
541 |
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1793772357762940928 |