Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota
Parasitic organisms inhabiting the alimentary canal should permanently resist the destructive action of host digestive enzymes. The intestinal parasites were shown to produce specific protease inhibitors protecting them from proteolysis. However, little is known about this adaptive mechanism in cest...
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ftczechacademysc:oai:kramerius.lib.cas.cz:uuid:2a99eccf-1f69-4006-88c1-e9ddac8dd75c 2024-03-17T08:57:14+00:00 Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota Izvekova, Galina I Frolova, Tatyana V Izvekov, Evgeny I 1-8 print počítač zdroj https://kramerius.lib.cas.cz/view/uuid:2a99eccf-1f69-4006-88c1-e9ddac8dd75c https://doi.org/10.14411/fp.2017.016 unknown https://kramerius.lib.cas.cz/view/uuid:2a99eccf-1f69-4006-88c1-e9ddac8dd75c issn:1803-6465 doi:10.14411/fp.2017.016 policy:public ryby trávicí enzymy inhibitory proteáz fishes digestive enzymes protease inhibitors helminths trypsin 2 59 model:article ftczechacademysc https://doi.org/10.14411/fp.2017.016 2024-02-19T22:58:13Z Parasitic organisms inhabiting the alimentary canal should permanently resist the destructive action of host digestive enzymes. The intestinal parasites were shown to produce specific protease inhibitors protecting them from proteolysis. However, little is known about this adaptive mechanism in cestodes so far, especially for the tapeworms dwelling inside the fish intestines. Here, we explored the ability to inactivate proteolytic enzymes in the fish tapeworm Eubothrium rugosum (Batsch, 1786) (Bothriocephalidea) parasitising the intestine of wild burbot, Lota lota (Linnaeus). The assays were conducted with different concentrations of commercial trypsin and homogenate of intestinal mucosa both being the sources of proteinases. The incubation of live E. rugosum in trypsin solutions of two different concentrations caused a significant decrease in the enzyme activity. The extent of activity reduction was dependent on trypsin concentration. At the same time, the inhibitory effect of the worm incubation medium turned out to be statistically insignificant. These findings suggest partial adsorption of the enzyme to the tegument surface, with its further inactivation. In contrast to the incubation medium, the worm extract suppressed over 80% of trypsin activity and nearly half of the proteolytic activity in the mucosa homogenate. Notably, the inhibitory activity of the tapeworms hardly depended on their size characteristics. Finally, the research has demonstrated secretion of proteinase inhibitor in E. rugosum, which appears to be essential for its survival in enzymatically hostile environment. Galina I. Izvekova, Tatyana V. Frolova, Evgeny I. Izvekov. Obsahuje bibliografii Article in Journal/Newspaper Burbot Lota lota lota Czech Academy of Sciences: dKNAV Frolova ENVELOPE(162.250,162.250,-70.667,-70.667) Folia Parasitologica 64 |
institution |
Open Polar |
collection |
Czech Academy of Sciences: dKNAV |
op_collection_id |
ftczechacademysc |
language |
unknown |
topic |
ryby trávicí enzymy inhibitory proteáz fishes digestive enzymes protease inhibitors helminths trypsin 2 59 |
spellingShingle |
ryby trávicí enzymy inhibitory proteáz fishes digestive enzymes protease inhibitors helminths trypsin 2 59 Izvekova, Galina I Frolova, Tatyana V Izvekov, Evgeny I Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota |
topic_facet |
ryby trávicí enzymy inhibitory proteáz fishes digestive enzymes protease inhibitors helminths trypsin 2 59 |
description |
Parasitic organisms inhabiting the alimentary canal should permanently resist the destructive action of host digestive enzymes. The intestinal parasites were shown to produce specific protease inhibitors protecting them from proteolysis. However, little is known about this adaptive mechanism in cestodes so far, especially for the tapeworms dwelling inside the fish intestines. Here, we explored the ability to inactivate proteolytic enzymes in the fish tapeworm Eubothrium rugosum (Batsch, 1786) (Bothriocephalidea) parasitising the intestine of wild burbot, Lota lota (Linnaeus). The assays were conducted with different concentrations of commercial trypsin and homogenate of intestinal mucosa both being the sources of proteinases. The incubation of live E. rugosum in trypsin solutions of two different concentrations caused a significant decrease in the enzyme activity. The extent of activity reduction was dependent on trypsin concentration. At the same time, the inhibitory effect of the worm incubation medium turned out to be statistically insignificant. These findings suggest partial adsorption of the enzyme to the tegument surface, with its further inactivation. In contrast to the incubation medium, the worm extract suppressed over 80% of trypsin activity and nearly half of the proteolytic activity in the mucosa homogenate. Notably, the inhibitory activity of the tapeworms hardly depended on their size characteristics. Finally, the research has demonstrated secretion of proteinase inhibitor in E. rugosum, which appears to be essential for its survival in enzymatically hostile environment. Galina I. Izvekova, Tatyana V. Frolova, Evgeny I. Izvekov. Obsahuje bibliografii |
format |
Article in Journal/Newspaper |
author |
Izvekova, Galina I Frolova, Tatyana V Izvekov, Evgeny I |
author_facet |
Izvekova, Galina I Frolova, Tatyana V Izvekov, Evgeny I |
author_sort |
Izvekova, Galina I |
title |
Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota |
title_short |
Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota |
title_full |
Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota |
title_fullStr |
Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota |
title_full_unstemmed |
Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota |
title_sort |
inactivation of proteolytic enzymes by eubothrium rugosum (cestoda) from the gut of burbot lota lota |
url |
https://kramerius.lib.cas.cz/view/uuid:2a99eccf-1f69-4006-88c1-e9ddac8dd75c https://doi.org/10.14411/fp.2017.016 |
op_coverage |
1-8 |
long_lat |
ENVELOPE(162.250,162.250,-70.667,-70.667) |
geographic |
Frolova |
geographic_facet |
Frolova |
genre |
Burbot Lota lota lota |
genre_facet |
Burbot Lota lota lota |
op_relation |
https://kramerius.lib.cas.cz/view/uuid:2a99eccf-1f69-4006-88c1-e9ddac8dd75c issn:1803-6465 doi:10.14411/fp.2017.016 |
op_rights |
policy:public |
op_doi |
https://doi.org/10.14411/fp.2017.016 |
container_title |
Folia Parasitologica |
container_volume |
64 |
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1793766318101495808 |