Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota

Parasitic organisms inhabiting the alimentary canal should permanently resist the destructive action of host digestive enzymes. The intestinal parasites were shown to produce specific protease inhibitors protecting them from proteolysis. However, little is known about this adaptive mechanism in cest...

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Published in:Folia Parasitologica
Main Authors: Izvekova, Galina I, Frolova, Tatyana V, Izvekov, Evgeny I
Format: Article in Journal/Newspaper
Language:unknown
Subjects:
2
59
Online Access:https://kramerius.lib.cas.cz/view/uuid:2a99eccf-1f69-4006-88c1-e9ddac8dd75c
https://doi.org/10.14411/fp.2017.016
id ftczechacademysc:oai:kramerius.lib.cas.cz:uuid:2a99eccf-1f69-4006-88c1-e9ddac8dd75c
record_format openpolar
spelling ftczechacademysc:oai:kramerius.lib.cas.cz:uuid:2a99eccf-1f69-4006-88c1-e9ddac8dd75c 2024-03-17T08:57:14+00:00 Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota Izvekova, Galina I Frolova, Tatyana V Izvekov, Evgeny I 1-8 print počítač zdroj https://kramerius.lib.cas.cz/view/uuid:2a99eccf-1f69-4006-88c1-e9ddac8dd75c https://doi.org/10.14411/fp.2017.016 unknown https://kramerius.lib.cas.cz/view/uuid:2a99eccf-1f69-4006-88c1-e9ddac8dd75c issn:1803-6465 doi:10.14411/fp.2017.016 policy:public ryby trávicí enzymy inhibitory proteáz fishes digestive enzymes protease inhibitors helminths trypsin 2 59 model:article ftczechacademysc https://doi.org/10.14411/fp.2017.016 2024-02-19T22:58:13Z Parasitic organisms inhabiting the alimentary canal should permanently resist the destructive action of host digestive enzymes. The intestinal parasites were shown to produce specific protease inhibitors protecting them from proteolysis. However, little is known about this adaptive mechanism in cestodes so far, especially for the tapeworms dwelling inside the fish intestines. Here, we explored the ability to inactivate proteolytic enzymes in the fish tapeworm Eubothrium rugosum (Batsch, 1786) (Bothriocephalidea) parasitising the intestine of wild burbot, Lota lota (Linnaeus). The assays were conducted with different concentrations of commercial trypsin and homogenate of intestinal mucosa both being the sources of proteinases. The incubation of live E. rugosum in trypsin solutions of two different concentrations caused a significant decrease in the enzyme activity. The extent of activity reduction was dependent on trypsin concentration. At the same time, the inhibitory effect of the worm incubation medium turned out to be statistically insignificant. These findings suggest partial adsorption of the enzyme to the tegument surface, with its further inactivation. In contrast to the incubation medium, the worm extract suppressed over 80% of trypsin activity and nearly half of the proteolytic activity in the mucosa homogenate. Notably, the inhibitory activity of the tapeworms hardly depended on their size characteristics. Finally, the research has demonstrated secretion of proteinase inhibitor in E. rugosum, which appears to be essential for its survival in enzymatically hostile environment. Galina I. Izvekova, Tatyana V. Frolova, Evgeny I. Izvekov. Obsahuje bibliografii Article in Journal/Newspaper Burbot Lota lota lota Czech Academy of Sciences: dKNAV Frolova ENVELOPE(162.250,162.250,-70.667,-70.667) Folia Parasitologica 64
institution Open Polar
collection Czech Academy of Sciences: dKNAV
op_collection_id ftczechacademysc
language unknown
topic ryby
trávicí enzymy
inhibitory proteáz
fishes
digestive enzymes
protease inhibitors
helminths
trypsin
2
59
spellingShingle ryby
trávicí enzymy
inhibitory proteáz
fishes
digestive enzymes
protease inhibitors
helminths
trypsin
2
59
Izvekova, Galina I
Frolova, Tatyana V
Izvekov, Evgeny I
Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota
topic_facet ryby
trávicí enzymy
inhibitory proteáz
fishes
digestive enzymes
protease inhibitors
helminths
trypsin
2
59
description Parasitic organisms inhabiting the alimentary canal should permanently resist the destructive action of host digestive enzymes. The intestinal parasites were shown to produce specific protease inhibitors protecting them from proteolysis. However, little is known about this adaptive mechanism in cestodes so far, especially for the tapeworms dwelling inside the fish intestines. Here, we explored the ability to inactivate proteolytic enzymes in the fish tapeworm Eubothrium rugosum (Batsch, 1786) (Bothriocephalidea) parasitising the intestine of wild burbot, Lota lota (Linnaeus). The assays were conducted with different concentrations of commercial trypsin and homogenate of intestinal mucosa both being the sources of proteinases. The incubation of live E. rugosum in trypsin solutions of two different concentrations caused a significant decrease in the enzyme activity. The extent of activity reduction was dependent on trypsin concentration. At the same time, the inhibitory effect of the worm incubation medium turned out to be statistically insignificant. These findings suggest partial adsorption of the enzyme to the tegument surface, with its further inactivation. In contrast to the incubation medium, the worm extract suppressed over 80% of trypsin activity and nearly half of the proteolytic activity in the mucosa homogenate. Notably, the inhibitory activity of the tapeworms hardly depended on their size characteristics. Finally, the research has demonstrated secretion of proteinase inhibitor in E. rugosum, which appears to be essential for its survival in enzymatically hostile environment. Galina I. Izvekova, Tatyana V. Frolova, Evgeny I. Izvekov. Obsahuje bibliografii
format Article in Journal/Newspaper
author Izvekova, Galina I
Frolova, Tatyana V
Izvekov, Evgeny I
author_facet Izvekova, Galina I
Frolova, Tatyana V
Izvekov, Evgeny I
author_sort Izvekova, Galina I
title Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota
title_short Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota
title_full Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota
title_fullStr Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota
title_full_unstemmed Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota
title_sort inactivation of proteolytic enzymes by eubothrium rugosum (cestoda) from the gut of burbot lota lota
url https://kramerius.lib.cas.cz/view/uuid:2a99eccf-1f69-4006-88c1-e9ddac8dd75c
https://doi.org/10.14411/fp.2017.016
op_coverage 1-8
long_lat ENVELOPE(162.250,162.250,-70.667,-70.667)
geographic Frolova
geographic_facet Frolova
genre Burbot
Lota lota
lota
genre_facet Burbot
Lota lota
lota
op_relation https://kramerius.lib.cas.cz/view/uuid:2a99eccf-1f69-4006-88c1-e9ddac8dd75c
issn:1803-6465
doi:10.14411/fp.2017.016
op_rights policy:public
op_doi https://doi.org/10.14411/fp.2017.016
container_title Folia Parasitologica
container_volume 64
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