Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake
To identify trematode diversity and life-cycles in the sub-Arctic Lake Takvatn, Norway, we characterised 120 trematode isolates from mollusc first intermediate hosts, metacercariae from second intermediate host fishes and invertebrates, and adults from fish and invertebrate definitive hosts, using m...
Published in: | International Journal for Parasitology |
---|---|
Main Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.ijpara.2016.12.008 http://hdl.handle.net/11104/0275244 |
id |
ftczacademyscien:oai:asep.lib.cas.cz:CavUnEpca/0479250 |
---|---|
record_format |
openpolar |
spelling |
ftczacademyscien:oai:asep.lib.cas.cz:CavUnEpca/0479250 2024-02-04T09:57:04+01:00 Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake Soldánová, M. (Miroslava) Georgieva, S. (Simona) Roháčová, J. (Jana) Knudsen, R. Kuhn, J. A. Henriksen, E. H. Siwertsson, A. Shaw, J. C. Kuris, A. M. Amundsen, P.-A. Scholz, T. (Tomáš) Lafferty, K. D. Kostadinova, A. (Aneta) 2017 https://doi.org/10.1016/j.ijpara.2016.12.008 http://hdl.handle.net/11104/0275244 eng eng info:eu-repo/semantics/altIdentifier/pmid/28315362 doi:10.1016/j.ijpara.2016.12.008 urn:pissn: 0020-7519 urn:eissn: 1879-0135 http://hdl.handle.net/11104/0275244 Trematode diversity intermediate hosts phylogeny mitochondrial DNA nuclear DNA Lake Takvatn Norway Sub-Arctic info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2017 ftczacademyscien https://doi.org/10.1016/j.ijpara.2016.12.008 2024-01-09T17:39:55Z To identify trematode diversity and life-cycles in the sub-Arctic Lake Takvatn, Norway, we characterised 120 trematode isolates from mollusc first intermediate hosts, metacercariae from second intermediate host fishes and invertebrates, and adults from fish and invertebrate definitive hosts, using molecular techniques. Phylogenies based on nuclear and/or mtDNA revealed high species richness (24 species or species-level genetic lineages) and uncovered trematode diversity (16 putative new species) from five families typical in lake ecosystems (Allocreadiidae, Diplostomidae, Plagiorchiidae, Schistosomatidae and Strigeidae). Sampling potential invertebrate hosts allowed matching of sequence data for different stages, thus achieving molecular elucidation of trematode life-cycles and exploration of host-parasite interactions. Phylogenetic analyses also helped identify three major mollusc intermediate hosts (Radix balthica, Pisidium casertanum and Sphaerium sp.) in the lake. Our findings increase the known trematode diversity at the sub-Arctic Lake Takvatn, showing that digenean diversity is high in this otherwise depauperate sub-Arctic freshwater ecosystem and indicating that sub-Arctic and Arctic ecosystems may be characterised by unique trematode assemblages. (C) 2017 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Arctic The Czech Academy of Sciences: Publication Activity (ASEP) Arctic Arctic Lake ENVELOPE(-130.826,-130.826,57.231,57.231) Norway International Journal for Parasitology 47 6 327 345 |
institution |
Open Polar |
collection |
The Czech Academy of Sciences: Publication Activity (ASEP) |
op_collection_id |
ftczacademyscien |
language |
English |
topic |
Trematode diversity intermediate hosts phylogeny mitochondrial DNA nuclear DNA Lake Takvatn Norway Sub-Arctic |
spellingShingle |
Trematode diversity intermediate hosts phylogeny mitochondrial DNA nuclear DNA Lake Takvatn Norway Sub-Arctic Soldánová, M. (Miroslava) Georgieva, S. (Simona) Roháčová, J. (Jana) Knudsen, R. Kuhn, J. A. Henriksen, E. H. Siwertsson, A. Shaw, J. C. Kuris, A. M. Amundsen, P.-A. Scholz, T. (Tomáš) Lafferty, K. D. Kostadinova, A. (Aneta) Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake |
topic_facet |
Trematode diversity intermediate hosts phylogeny mitochondrial DNA nuclear DNA Lake Takvatn Norway Sub-Arctic |
description |
To identify trematode diversity and life-cycles in the sub-Arctic Lake Takvatn, Norway, we characterised 120 trematode isolates from mollusc first intermediate hosts, metacercariae from second intermediate host fishes and invertebrates, and adults from fish and invertebrate definitive hosts, using molecular techniques. Phylogenies based on nuclear and/or mtDNA revealed high species richness (24 species or species-level genetic lineages) and uncovered trematode diversity (16 putative new species) from five families typical in lake ecosystems (Allocreadiidae, Diplostomidae, Plagiorchiidae, Schistosomatidae and Strigeidae). Sampling potential invertebrate hosts allowed matching of sequence data for different stages, thus achieving molecular elucidation of trematode life-cycles and exploration of host-parasite interactions. Phylogenetic analyses also helped identify three major mollusc intermediate hosts (Radix balthica, Pisidium casertanum and Sphaerium sp.) in the lake. Our findings increase the known trematode diversity at the sub-Arctic Lake Takvatn, showing that digenean diversity is high in this otherwise depauperate sub-Arctic freshwater ecosystem and indicating that sub-Arctic and Arctic ecosystems may be characterised by unique trematode assemblages. (C) 2017 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Soldánová, M. (Miroslava) Georgieva, S. (Simona) Roháčová, J. (Jana) Knudsen, R. Kuhn, J. A. Henriksen, E. H. Siwertsson, A. Shaw, J. C. Kuris, A. M. Amundsen, P.-A. Scholz, T. (Tomáš) Lafferty, K. D. Kostadinova, A. (Aneta) |
author_facet |
Soldánová, M. (Miroslava) Georgieva, S. (Simona) Roháčová, J. (Jana) Knudsen, R. Kuhn, J. A. Henriksen, E. H. Siwertsson, A. Shaw, J. C. Kuris, A. M. Amundsen, P.-A. Scholz, T. (Tomáš) Lafferty, K. D. Kostadinova, A. (Aneta) |
author_sort |
Soldánová, M. (Miroslava) |
title |
Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake |
title_short |
Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake |
title_full |
Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake |
title_fullStr |
Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake |
title_full_unstemmed |
Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake |
title_sort |
molecular analyses reveal high species diversity of trematodes in a sub-arctic lake |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.ijpara.2016.12.008 http://hdl.handle.net/11104/0275244 |
long_lat |
ENVELOPE(-130.826,-130.826,57.231,57.231) |
geographic |
Arctic Arctic Lake Norway |
geographic_facet |
Arctic Arctic Lake Norway |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/28315362 doi:10.1016/j.ijpara.2016.12.008 urn:pissn: 0020-7519 urn:eissn: 1879-0135 http://hdl.handle.net/11104/0275244 |
op_doi |
https://doi.org/10.1016/j.ijpara.2016.12.008 |
container_title |
International Journal for Parasitology |
container_volume |
47 |
container_issue |
6 |
container_start_page |
327 |
op_container_end_page |
345 |
_version_ |
1789961383677263872 |