Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation
In sturgeon, the acquisition of the potential for motility activation called spermatozoon maturation takes place outside testes. This process can be accomplished in vitro by pre-incubation of immature testicular spermatozoa in seminal fluid collected from fully mature Wolffian duct sperm. Addition o...
Published in: | Fish Physiology and Biochemistry |
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2016
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Online Access: | https://doi.org/10.1007/s10695-016-0255-x http://hdl.handle.net/11104/0266561 |
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ftczacademyscien:oai:asep.lib.cas.cz:CavUnEpca/0468729 2024-02-04T10:04:30+01:00 Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation Dzyuba, V. Słowińska, M. Cosson, J. Ciereszko, A. Boryshpolets, S. Štěrba, J. (Ján) Rodina, M. Linhart, O. Dzyuba, B. 2016 https://doi.org/10.1007/s10695-016-0255-x http://hdl.handle.net/11104/0266561 eng eng info:eu-repo/semantics/altIdentifier/pmid/27401784 doi:10.1007/s10695-016-0255-x urn:pissn: 0920-1742 urn:eissn: 1573-5168 http://hdl.handle.net/11104/0266561 amidase activity anti-proteolytic activity casein and gelatin zymography siberian sturgeon sperm spermatozoon maturation sterlet sperm info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2016 ftczacademyscien https://doi.org/10.1007/s10695-016-0255-x 2024-01-09T17:37:28Z In sturgeon, the acquisition of the potential for motility activation called spermatozoon maturation takes place outside testes. This process can be accomplished in vitro by pre-incubation of immature testicular spermatozoa in seminal fluid collected from fully mature Wolffian duct sperm. Addition of trypsin inhibitor to the pre-incubation medium disrupts spermatozoon maturation. There are no available data for the role of proteolysis regulators in fish spermatozoon maturation, while their role is recognized in mammalian sperm maturation. The present study evaluated the involvement of seminal fluid proteases and anti-proteolytic activity in the sterlet spermatozoon maturation process. Casein and gelatin zymography and quantification of amidase and anti-proteolytic activity were conducted in sturgeon seminal fluid from Wolffian duct sperm and seminal fluid from testicular sperm, along with spermatozoon extracts from Wolffian duct spermatozoa, testicular spermatozoa, and testicular spermatozoa after in vitro maturation. We did not find significant differences in proteolytic profiles of seminal fluids from Wolffian duct sperm and ones from testicular sperm. Zymography revealed differences in spermatozoon extracts: Wolffian duct spermatozoon extracts were characterized by the presence of a broad proteolytic band ranging from 48 to 41 kDa, while testicular spermatozoon extracts did not show such activity until after in vitro maturation. The differences in amidase activity coincided with these results. It may not be the levels of proteolytic and anti-proteolytic activity per se, but the alterations in their interactions triggering a cascade of signaling events, that is crucial to the maturation process. Article in Journal/Newspaper Siberian sturgeon The Czech Academy of Sciences: Publication Activity (ASEP) Fish Physiology and Biochemistry 42 6 1755 1766 |
institution |
Open Polar |
collection |
The Czech Academy of Sciences: Publication Activity (ASEP) |
op_collection_id |
ftczacademyscien |
language |
English |
topic |
amidase activity anti-proteolytic activity casein and gelatin zymography siberian sturgeon sperm spermatozoon maturation sterlet sperm |
spellingShingle |
amidase activity anti-proteolytic activity casein and gelatin zymography siberian sturgeon sperm spermatozoon maturation sterlet sperm Dzyuba, V. Słowińska, M. Cosson, J. Ciereszko, A. Boryshpolets, S. Štěrba, J. (Ján) Rodina, M. Linhart, O. Dzyuba, B. Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation |
topic_facet |
amidase activity anti-proteolytic activity casein and gelatin zymography siberian sturgeon sperm spermatozoon maturation sterlet sperm |
description |
In sturgeon, the acquisition of the potential for motility activation called spermatozoon maturation takes place outside testes. This process can be accomplished in vitro by pre-incubation of immature testicular spermatozoa in seminal fluid collected from fully mature Wolffian duct sperm. Addition of trypsin inhibitor to the pre-incubation medium disrupts spermatozoon maturation. There are no available data for the role of proteolysis regulators in fish spermatozoon maturation, while their role is recognized in mammalian sperm maturation. The present study evaluated the involvement of seminal fluid proteases and anti-proteolytic activity in the sterlet spermatozoon maturation process. Casein and gelatin zymography and quantification of amidase and anti-proteolytic activity were conducted in sturgeon seminal fluid from Wolffian duct sperm and seminal fluid from testicular sperm, along with spermatozoon extracts from Wolffian duct spermatozoa, testicular spermatozoa, and testicular spermatozoa after in vitro maturation. We did not find significant differences in proteolytic profiles of seminal fluids from Wolffian duct sperm and ones from testicular sperm. Zymography revealed differences in spermatozoon extracts: Wolffian duct spermatozoon extracts were characterized by the presence of a broad proteolytic band ranging from 48 to 41 kDa, while testicular spermatozoon extracts did not show such activity until after in vitro maturation. The differences in amidase activity coincided with these results. It may not be the levels of proteolytic and anti-proteolytic activity per se, but the alterations in their interactions triggering a cascade of signaling events, that is crucial to the maturation process. |
format |
Article in Journal/Newspaper |
author |
Dzyuba, V. Słowińska, M. Cosson, J. Ciereszko, A. Boryshpolets, S. Štěrba, J. (Ján) Rodina, M. Linhart, O. Dzyuba, B. |
author_facet |
Dzyuba, V. Słowińska, M. Cosson, J. Ciereszko, A. Boryshpolets, S. Štěrba, J. (Ján) Rodina, M. Linhart, O. Dzyuba, B. |
author_sort |
Dzyuba, V. |
title |
Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation |
title_short |
Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation |
title_full |
Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation |
title_fullStr |
Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation |
title_full_unstemmed |
Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation |
title_sort |
characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation |
publishDate |
2016 |
url |
https://doi.org/10.1007/s10695-016-0255-x http://hdl.handle.net/11104/0266561 |
genre |
Siberian sturgeon |
genre_facet |
Siberian sturgeon |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/27401784 doi:10.1007/s10695-016-0255-x urn:pissn: 0920-1742 urn:eissn: 1573-5168 http://hdl.handle.net/11104/0266561 |
op_doi |
https://doi.org/10.1007/s10695-016-0255-x |
container_title |
Fish Physiology and Biochemistry |
container_volume |
42 |
container_issue |
6 |
container_start_page |
1755 |
op_container_end_page |
1766 |
_version_ |
1789973007182069760 |