Lipidomic analysis of psychrophilic yeasts cultivated at different temperatures

• Published in: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
• Main Authors: Řezanka, T. (Tomáš), Kolouchová, I., Sigler, K. (Karel)
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2016
• Subjects: Lipidomic analysis; Phosphatidylcholine; Phosphatidylethanolamine; Cryptococcus victoriae
• Online Access: https://doi.org/10.1016/j.bbalip.2016.07.005; http://hdl.handle.net/11104/0262323

Analysis of polar lipids from eight psychrophilic yeasts (Cryptococcus victoriae, Cystofilobasidium capitatum, Holtermaniella wattica, Mrakiella aquatica, M. cryoconiti, Rhodotorula lignophila, Kondoa malvinella and Trichosporon aggtelekiense) grown at 4–28 C by hydrophilic interaction liquid chromatography/high resolution electrospray ionization tandem mass spectrometry determined 17 classes of lipids and identified dozens of molecular species of phospholipids including their regioisomers. Most of the yeasts were able to grow over the whole temperature range, reaching the highest biomass at 4 or 10 C. On temperature drop to 4 C, all eight strains showed a significant decrease of MUFA and a simultaneous increase of PUFA such as α-linolenic acid, the content of which in the biomass reached up to 20 percent.\n