Metabolic pathways of benzimidazole anthelmintics in harebell (Campanula rotundifolia)
Benzimidazoles anthelmintics, which enter into environment primarily through excretion in the feces or urine of treated animals, can affect various organisms and disrupt ecosystem balance. The present study was designed to test the phytotoxicity and biotransformation of the three benzimidazole anthe...
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ftczacademyscien:oai:asep.lib.cas.cz:CavUnEpca/0461920 2024-02-04T09:59:27+01:00 Metabolic pathways of benzimidazole anthelmintics in harebell (Campanula rotundifolia) Stuchlíková, L. Jirásko, R. Skálová, L. Pavlík, F. Szotáková, B. Holčapek, M. Vaněk, T. (Tomáš) Podlipná, R. (Radka) 2016 https://doi.org/10.1016/j.chemosphere.2016.05.015 http://hdl.handle.net/11104/0261487 eng eng doi:10.1016/j.chemosphere.2016.05.015 urn:pissn: 0045-6535 urn:eissn: 1879-1298 http://hdl.handle.net/11104/0261487 info:eu-repo/semantics/openAccess Drug metabolism Biotransformation Albendazole info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2016 ftczacademyscien https://doi.org/10.1016/j.chemosphere.2016.05.015 2024-01-09T17:35:51Z Benzimidazoles anthelmintics, which enter into environment primarily through excretion in the feces or urine of treated animals, can affect various organisms and disrupt ecosystem balance. The present study was designed to test the phytotoxicity and biotransformation of the three benzimidazole anthelmintics albendazole (ABZ), fenbendazole (FBZ) and flubendazole (FLU) in the harebell (Campanula rotundifolia). This meadow plant commonly grows in pastures and comes into contact with anthelmintics through the excrements of treated animals. Suspensions of harebell cells in culture medium were used as an in vitro model system. ABZ, FLU and FBZ were not found to be toxic for harebell cells, which were able to metabolize ABZ, FLU and FBZ via the formation of a wide scale of metabolites. Ultrahigh-performance liquid chromatography coupled with high mass accuracy tandem mass spectrometry (UHPLC-MS/MS) led to the identification of 24, 18 and 29 metabolites of ABZ, FLU and FBZ, respectively. Several novel metabolites were identified for the first time. Based on the obtained results, the schemes of the metabolic pathways of these anthelmintics were proposed. Most of these metabolites can be considered deactivation products, but a substantial portion of them may readily be decomposed to biologically active substances which could negatively affect ecosystems. Article in Journal/Newspaper Campanula rotundifolia Harebell The Czech Academy of Sciences: Publication Activity (ASEP) Chemosphere 157 10 17 |
institution |
Open Polar |
collection |
The Czech Academy of Sciences: Publication Activity (ASEP) |
op_collection_id |
ftczacademyscien |
language |
English |
topic |
Drug metabolism Biotransformation Albendazole |
spellingShingle |
Drug metabolism Biotransformation Albendazole Stuchlíková, L. Jirásko, R. Skálová, L. Pavlík, F. Szotáková, B. Holčapek, M. Vaněk, T. (Tomáš) Podlipná, R. (Radka) Metabolic pathways of benzimidazole anthelmintics in harebell (Campanula rotundifolia) |
topic_facet |
Drug metabolism Biotransformation Albendazole |
description |
Benzimidazoles anthelmintics, which enter into environment primarily through excretion in the feces or urine of treated animals, can affect various organisms and disrupt ecosystem balance. The present study was designed to test the phytotoxicity and biotransformation of the three benzimidazole anthelmintics albendazole (ABZ), fenbendazole (FBZ) and flubendazole (FLU) in the harebell (Campanula rotundifolia). This meadow plant commonly grows in pastures and comes into contact with anthelmintics through the excrements of treated animals. Suspensions of harebell cells in culture medium were used as an in vitro model system. ABZ, FLU and FBZ were not found to be toxic for harebell cells, which were able to metabolize ABZ, FLU and FBZ via the formation of a wide scale of metabolites. Ultrahigh-performance liquid chromatography coupled with high mass accuracy tandem mass spectrometry (UHPLC-MS/MS) led to the identification of 24, 18 and 29 metabolites of ABZ, FLU and FBZ, respectively. Several novel metabolites were identified for the first time. Based on the obtained results, the schemes of the metabolic pathways of these anthelmintics were proposed. Most of these metabolites can be considered deactivation products, but a substantial portion of them may readily be decomposed to biologically active substances which could negatively affect ecosystems. |
format |
Article in Journal/Newspaper |
author |
Stuchlíková, L. Jirásko, R. Skálová, L. Pavlík, F. Szotáková, B. Holčapek, M. Vaněk, T. (Tomáš) Podlipná, R. (Radka) |
author_facet |
Stuchlíková, L. Jirásko, R. Skálová, L. Pavlík, F. Szotáková, B. Holčapek, M. Vaněk, T. (Tomáš) Podlipná, R. (Radka) |
author_sort |
Stuchlíková, L. |
title |
Metabolic pathways of benzimidazole anthelmintics in harebell (Campanula rotundifolia) |
title_short |
Metabolic pathways of benzimidazole anthelmintics in harebell (Campanula rotundifolia) |
title_full |
Metabolic pathways of benzimidazole anthelmintics in harebell (Campanula rotundifolia) |
title_fullStr |
Metabolic pathways of benzimidazole anthelmintics in harebell (Campanula rotundifolia) |
title_full_unstemmed |
Metabolic pathways of benzimidazole anthelmintics in harebell (Campanula rotundifolia) |
title_sort |
metabolic pathways of benzimidazole anthelmintics in harebell (campanula rotundifolia) |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.chemosphere.2016.05.015 http://hdl.handle.net/11104/0261487 |
genre |
Campanula rotundifolia Harebell |
genre_facet |
Campanula rotundifolia Harebell |
op_relation |
doi:10.1016/j.chemosphere.2016.05.015 urn:pissn: 0045-6535 urn:eissn: 1879-1298 http://hdl.handle.net/11104/0261487 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.chemosphere.2016.05.015 |
container_title |
Chemosphere |
container_volume |
157 |
container_start_page |
10 |
op_container_end_page |
17 |
_version_ |
1789964249840222208 |