Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences
We applied comparative genomic hybridization (CGH) and genomic in situ hybridization (GISH) to examine genomes of artificially produced sturgeon hybrids between sterlet, Acipenser ruthenus female (120 chromosomes) or Russian sturgeon, A. gueldenstaedtii female (240 chromosomes) and a spontaneous tri...
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Online Access: | https://doi.org/10.1159/000354882 http://hdl.handle.net/11104/0225451 |
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ftczacademyscien:oai:asep.lib.cas.cz:CavUnEpca/0397793 2024-02-04T10:04:30+01:00 Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences Symonová, R. (Radka) Flajšhans, M. Sember, A. (Alexandr) Havelka, M. Gela, D. Kořínková, T. (Tereza) Rodina, M. Rábová, M. (Marie) Ráb, P. (Petr) 2013 https://doi.org/10.1159/000354882 http://hdl.handle.net/11104/0225451 eng eng doi:10.1159/000354882 urn:pissn: 1424-8581 urn:eissn: 1424-859x http://hdl.handle.net/11104/0225451 Acipenser GISH Hybridization Macrochromosomes info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2013 ftczacademyscien https://doi.org/10.1159/000354882 2024-01-09T17:25:35Z We applied comparative genomic hybridization (CGH) and genomic in situ hybridization (GISH) to examine genomes of artificially produced sturgeon hybrids between sterlet, Acipenser ruthenus female (120 chromosomes) or Russian sturgeon, A. gueldenstaedtii female (240 chromosomes) and a spontaneous triploid Siberian sturgeon A. baerii male (360 chromosomes), respectively. The ploidy levels of progenies were analyzed by karyotyping and flow cytometry. We found that the species-specific regions were surprisingly identifiable only on some micro- and small(er) macrochromosomes in hybrid metaphases. We hypothesize that these distinguishable regions are represented by species-specific repetitive sequences driven by more dynamic molecular evolutionary mechanisms. On larger chromosomes, GISH faintly visualized only blocks of pericentromeric and telomeric repetitive sequences, remaining regions were equally shared by both parental species. We concluded that the interspecies hybridization producing viable and even fertile progeny is enabled by the fact that genomes of the species involved are likely divergent at the level of the repetitive sequences only and probably highly conserved in the coding sequences. These small differences of coding sequences are in concordance with previous estimations of relatedness of examined species producing artificial as well as natural hybrids. CGH and GISH represent a challenge in sturgeon cytogenetics as a valuable though technically not simple tool to discriminate chromosomes of parental species in hybrids. The potentials and drawbacks of CGH and GISH application in sturgeons are discussed and further experimental possibilities are proposed. Article in Journal/Newspaper Siberian sturgeon The Czech Academy of Sciences: Publication Activity (ASEP) Cytogenetic and Genome Research 141 2-3 153 162 |
institution |
Open Polar |
collection |
The Czech Academy of Sciences: Publication Activity (ASEP) |
op_collection_id |
ftczacademyscien |
language |
English |
topic |
Acipenser GISH Hybridization Macrochromosomes |
spellingShingle |
Acipenser GISH Hybridization Macrochromosomes Symonová, R. (Radka) Flajšhans, M. Sember, A. (Alexandr) Havelka, M. Gela, D. Kořínková, T. (Tereza) Rodina, M. Rábová, M. (Marie) Ráb, P. (Petr) Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences |
topic_facet |
Acipenser GISH Hybridization Macrochromosomes |
description |
We applied comparative genomic hybridization (CGH) and genomic in situ hybridization (GISH) to examine genomes of artificially produced sturgeon hybrids between sterlet, Acipenser ruthenus female (120 chromosomes) or Russian sturgeon, A. gueldenstaedtii female (240 chromosomes) and a spontaneous triploid Siberian sturgeon A. baerii male (360 chromosomes), respectively. The ploidy levels of progenies were analyzed by karyotyping and flow cytometry. We found that the species-specific regions were surprisingly identifiable only on some micro- and small(er) macrochromosomes in hybrid metaphases. We hypothesize that these distinguishable regions are represented by species-specific repetitive sequences driven by more dynamic molecular evolutionary mechanisms. On larger chromosomes, GISH faintly visualized only blocks of pericentromeric and telomeric repetitive sequences, remaining regions were equally shared by both parental species. We concluded that the interspecies hybridization producing viable and even fertile progeny is enabled by the fact that genomes of the species involved are likely divergent at the level of the repetitive sequences only and probably highly conserved in the coding sequences. These small differences of coding sequences are in concordance with previous estimations of relatedness of examined species producing artificial as well as natural hybrids. CGH and GISH represent a challenge in sturgeon cytogenetics as a valuable though technically not simple tool to discriminate chromosomes of parental species in hybrids. The potentials and drawbacks of CGH and GISH application in sturgeons are discussed and further experimental possibilities are proposed. |
format |
Article in Journal/Newspaper |
author |
Symonová, R. (Radka) Flajšhans, M. Sember, A. (Alexandr) Havelka, M. Gela, D. Kořínková, T. (Tereza) Rodina, M. Rábová, M. (Marie) Ráb, P. (Petr) |
author_facet |
Symonová, R. (Radka) Flajšhans, M. Sember, A. (Alexandr) Havelka, M. Gela, D. Kořínková, T. (Tereza) Rodina, M. Rábová, M. (Marie) Ráb, P. (Petr) |
author_sort |
Symonová, R. (Radka) |
title |
Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences |
title_short |
Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences |
title_full |
Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences |
title_fullStr |
Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences |
title_full_unstemmed |
Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences |
title_sort |
molecular cytogenetics in artificial hybrid and highly polyploid sturgeons: an evolutionary story narrated by repetitive sequences |
publishDate |
2013 |
url |
https://doi.org/10.1159/000354882 http://hdl.handle.net/11104/0225451 |
genre |
Siberian sturgeon |
genre_facet |
Siberian sturgeon |
op_relation |
doi:10.1159/000354882 urn:pissn: 1424-8581 urn:eissn: 1424-859x http://hdl.handle.net/11104/0225451 |
op_doi |
https://doi.org/10.1159/000354882 |
container_title |
Cytogenetic and Genome Research |
container_volume |
141 |
container_issue |
2-3 |
container_start_page |
153 |
op_container_end_page |
162 |
_version_ |
1789973006998568960 |