Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism

This paper shows that mechanisms of freeze-tolerance studied by us previously in subarctic fly Chymomyza costata can be applied to a fruit fly of tropical origin with a weak innate capacity to tolerate even mild chilling. We found that surprisingly simple laboratory manipulations can change the chil...

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Published in:Proceedings of the National Academy of Sciences
Main Authors: Košťál, V. (Vladimír), Šimek, P. (Petr), Zahradníčková, H. (Helena), Cimlová, J. (Jana), Štětina, T.
Format: Article in Journal/Newspaper
Language:English
Published: 2012
Subjects:
Online Access:https://doi.org/10.1073/pnas.1119986109
http://hdl.handle.net/11104/0208325
id ftczacademyscien:oai:asep.lib.cas.cz:CavUnEpca/0375752
record_format openpolar
spelling ftczacademyscien:oai:asep.lib.cas.cz:CavUnEpca/0375752 2024-02-11T10:08:59+01:00 Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism Košťál, V. (Vladimír) Šimek, P. (Petr) Zahradníčková, H. (Helena) Cimlová, J. (Jana) Štětina, T. 2012 https://doi.org/10.1073/pnas.1119986109 http://hdl.handle.net/11104/0208325 eng eng doi:10.1073/pnas.1119986109 urn:pissn: 0027-8424 http://hdl.handle.net/11104/0208325 insect cold tolerance long-term storage metabolomics info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2012 ftczacademyscien https://doi.org/10.1073/pnas.1119986109 2024-01-16T17:15:08Z This paper shows that mechanisms of freeze-tolerance studied by us previously in subarctic fly Chymomyza costata can be applied to a fruit fly of tropical origin with a weak innate capacity to tolerate even mild chilling. We found that surprisingly simple laboratory manipulations can change the chill susceptible insect to the freeze-tolerant one. Larvae of Drosophila melanogaster can then survive at subzero temperatures when approximately 50% of their body water turns to ice crystals. To achieve this goal, synergy of two fundamental prerequisites is required: (a) shutdown of larval development, including all the chill sensitive processes linked to it, by exposing larvae to low but above-lethal temperatures (quiescence), and (b) incorporating the free amino acid proline in tissues by feeding larvae a proline-augmented diet (cryopreservation). Article in Journal/Newspaper Subarctic The Czech Academy of Sciences: Publication Activity (ASEP) Proceedings of the National Academy of Sciences 109 9 3270 3274
institution Open Polar
collection The Czech Academy of Sciences: Publication Activity (ASEP)
op_collection_id ftczacademyscien
language English
topic insect cold tolerance
long-term storage
metabolomics
spellingShingle insect cold tolerance
long-term storage
metabolomics
Košťál, V. (Vladimír)
Šimek, P. (Petr)
Zahradníčková, H. (Helena)
Cimlová, J. (Jana)
Štětina, T.
Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism
topic_facet insect cold tolerance
long-term storage
metabolomics
description This paper shows that mechanisms of freeze-tolerance studied by us previously in subarctic fly Chymomyza costata can be applied to a fruit fly of tropical origin with a weak innate capacity to tolerate even mild chilling. We found that surprisingly simple laboratory manipulations can change the chill susceptible insect to the freeze-tolerant one. Larvae of Drosophila melanogaster can then survive at subzero temperatures when approximately 50% of their body water turns to ice crystals. To achieve this goal, synergy of two fundamental prerequisites is required: (a) shutdown of larval development, including all the chill sensitive processes linked to it, by exposing larvae to low but above-lethal temperatures (quiescence), and (b) incorporating the free amino acid proline in tissues by feeding larvae a proline-augmented diet (cryopreservation).
format Article in Journal/Newspaper
author Košťál, V. (Vladimír)
Šimek, P. (Petr)
Zahradníčková, H. (Helena)
Cimlová, J. (Jana)
Štětina, T.
author_facet Košťál, V. (Vladimír)
Šimek, P. (Petr)
Zahradníčková, H. (Helena)
Cimlová, J. (Jana)
Štětina, T.
author_sort Košťál, V. (Vladimír)
title Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism
title_short Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism
title_full Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism
title_fullStr Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism
title_full_unstemmed Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism
title_sort conversion of the chill susceptible fruit fly larva (drosophila melanogaster) to a freeze tolerant organism
publishDate 2012
url https://doi.org/10.1073/pnas.1119986109
http://hdl.handle.net/11104/0208325
genre Subarctic
genre_facet Subarctic
op_relation doi:10.1073/pnas.1119986109
urn:pissn: 0027-8424
http://hdl.handle.net/11104/0208325
op_doi https://doi.org/10.1073/pnas.1119986109
container_title Proceedings of the National Academy of Sciences
container_volume 109
container_issue 9
container_start_page 3270
op_container_end_page 3274
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