РЕАКЦИЯ ИММУНОФИЗИОЛОГИЧЕСКИХ ПОКАЗАТЕЛЕЙ МОЛОДИ СИБИРСКОГО ОСЕТРА (ACIPENSER BAERII BRANDT) НА ДЕЙСТВИЕ ПЕРМЕТРИНА

• Main Author: Лапирова, Татьяна
• Format: Text
• Language: unknown
• Published: Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Национальный исследовательский Томский государственный университет" 2011
• Subjects: ПЕРМЕТРИН; МОЛОДЬ ОСЕТРА; ИММУНОФИЗИОЛОГИЧЕСКИЕ ПОКАЗАТЕЛИ; ХРОНИЧЕСКИЙ ЭКСПЕРИМЕНТ; Acipenser baerii; Siberian sturgeon
• Online Access: http://cyberleninka.ru/article/n/reaktsiya-immunofiziologicheskih-pokazateley-molodi-sibirskogo-osetra-acipenser-baerii-brandt-na-deystvie-permetrina; http://cyberleninka.ru/article_covers/13987389.png

Изучено влияние пестицида перметрина на некоторые морфофизиологические и иммунологические показатели молоди сибирского осетра в хроническом эксперименте. Показано, что даже низкие сублетальные концентрации пестицида (0,1 мкг/л) вызывают значительные колебания всех исследованных параметров. Выявленные изменения свидетельствуют о сдвигах в функционировании печени, нарушении белкового обмена, угнетении защитных функций организма. The paper studies the influence of the pesticide permethrin (concentration 0,1 μg/L, which accounted for 0.1 of the established 96-hour. LC50) on some morphophysiological and immunological parameters of Siberian sturgeon fingerling in a chronic experiment. The results of the experiment revealed active participation of all investigated immunocompetent organs (heart, spleen, liver) in response to the toxic effects. Fluctuations in the relative masses found for all organs, the most obvious changes were observed in the values of hepatosomatic index, which were significantly lower than the controls at the end of the observation period (8 weeks). A hepatotoxic effect of the pesticide is also confirmed by the decrease in the level of total serum protein, detected in the experimental fish. Proteinsynthesis oppression, in turn, is one of the reasons for humoral protection suppression. Another important reason for reducing the production of innate immunity humoral factors may be a malfunction of leukopoiesis processes, caused by the toxic effect of pesticide on immunocompetent organs. The combination of these effects could lead to a decrease in protective properties of serum. This statement is confirmed by our data on the complete suppression of blood serum bacteriostatic properties at the end of the observation period. Besides the shifts in relative mass of immunocompetent organs, in their tissues there were noticed fluctuations in the concentration of lysozyme. Maximum excess against control values was revealed during the first sampling period in the extracts from tissues of heart and spleen, which had the largest concentrations of lymphomyeloid tissue about 8 and 7 times respectively. Such a sharp rise in the concentration of lysozyme can lead subsequently to the depletion of resources of the humoral protection and persistent reduction in the level of enzyme in tissues. Generally, the observed changes are characteristic of nonspecific stress response case. It is noteworthy that by the end of the experiment not all of the tested parameters had recovered to control values (as this happens during the adaptation phase), which is indicative of intense violation of homeostatic mechanisms. The obtained data supports the conclusion of high toxicity of permethrin for the fingerling sturgeon, even when exposed to low sublethal concentrations.