The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica
Copyright: 2012 Springer Verlag. This is an ABSTRACT ONLY. The definitive version is published in Polar Biology, vol. 35(8), pp 1129-1141 Marion Island is a Sub-Antarctic island made up of distinct ecological habitats based on soil physiochemical, plant cover and physical characteristics. The microb...
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ftcsir:oai:researchspace.csir.co.za:10204/6697 2023-05-15T13:35:51+02:00 The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica Sanyika, TW Stafford, William HL Cowan, DA 2012-08 application/pdf http://hdl.handle.net/10204/6697 http://link.springer.com/content/pdf/10.1007/s00300-012-1160-0 en eng Springer Verlag Workflow;9377 Sanyika, T.W, Stafford, W, Cowan, D.A. 2012. The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica. Polar Biology, vol. 35(8), pp 1129-1141 0722-4060 http://link.springer.com/content/pdf/10.1007/s00300-012-1160-0 http://hdl.handle.net/10204/6697 Actinobacteria Marion Island terrestrial habitats Soil determinants Plant determinants Microbial diversity Sub-Antarctic environment Article 2012 ftcsir 2022-05-19T06:15:26Z Copyright: 2012 Springer Verlag. This is an ABSTRACT ONLY. The definitive version is published in Polar Biology, vol. 35(8), pp 1129-1141 Marion Island is a Sub-Antarctic island made up of distinct ecological habitats based on soil physiochemical, plant cover and physical characteristics. The microbial diversity and ecological determinants in this harsh Sub-Antarctic environment are largely uncharacterized. Actinobacteria have diverse ecological functions related to soil and plant functioning. This study was aimed at characterizing the diversity and community structures of the dominant actinobacteria in the distinct habitats and to identify their determinant soil and plant characteristics. Using the 16S rRNA gene, the denaturing gradient gel electrophoresis patterns and clone library diversity were correlated with the soil and plant characteristics. Multivariate statistical methods were also used to identify determinant soil and plant characteristics. Salinity and pH were the most important soil determinants, and a number of important site-specific plant species may have been important. The Coastal Fellfield Habitat was dominated by sequences of the suborders Micrococcineae (44%) and Propionibacterineae (18%), with salinity identified as the principal determinant. The Cotula Herbfield Habitat was dominated by Frankineae (37%) and Streptosporangineae (38%), which were correlated with organic nutrient concentrations. The Wet Mire Habitat was dominated by Acidimicrobineae (61%), with moisture and organic carbon content as principal components. Culture-dependent studies were complementary to culture-independent studies with the majority of actinobacteria isolated not identified in 16S rRNA gene clone libraries. This study demonstrates how the soil physiochemical characteristics and plant species independently determine the community structures of the dominant actinobacteria in distinct ecological habitats. These factors subsequently influence their ecological adaptation, roles and functions. Article in Journal/Newspaper Antarc* Antarctic Antarctica Marion Island Polar Biology Council for Scientific and Industrial Research (South Africa): CSIR Research Space Antarctic |
institution |
Open Polar |
collection |
Council for Scientific and Industrial Research (South Africa): CSIR Research Space |
op_collection_id |
ftcsir |
language |
English |
topic |
Actinobacteria Marion Island terrestrial habitats Soil determinants Plant determinants Microbial diversity Sub-Antarctic environment |
spellingShingle |
Actinobacteria Marion Island terrestrial habitats Soil determinants Plant determinants Microbial diversity Sub-Antarctic environment Sanyika, TW Stafford, William HL Cowan, DA The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica |
topic_facet |
Actinobacteria Marion Island terrestrial habitats Soil determinants Plant determinants Microbial diversity Sub-Antarctic environment |
description |
Copyright: 2012 Springer Verlag. This is an ABSTRACT ONLY. The definitive version is published in Polar Biology, vol. 35(8), pp 1129-1141 Marion Island is a Sub-Antarctic island made up of distinct ecological habitats based on soil physiochemical, plant cover and physical characteristics. The microbial diversity and ecological determinants in this harsh Sub-Antarctic environment are largely uncharacterized. Actinobacteria have diverse ecological functions related to soil and plant functioning. This study was aimed at characterizing the diversity and community structures of the dominant actinobacteria in the distinct habitats and to identify their determinant soil and plant characteristics. Using the 16S rRNA gene, the denaturing gradient gel electrophoresis patterns and clone library diversity were correlated with the soil and plant characteristics. Multivariate statistical methods were also used to identify determinant soil and plant characteristics. Salinity and pH were the most important soil determinants, and a number of important site-specific plant species may have been important. The Coastal Fellfield Habitat was dominated by sequences of the suborders Micrococcineae (44%) and Propionibacterineae (18%), with salinity identified as the principal determinant. The Cotula Herbfield Habitat was dominated by Frankineae (37%) and Streptosporangineae (38%), which were correlated with organic nutrient concentrations. The Wet Mire Habitat was dominated by Acidimicrobineae (61%), with moisture and organic carbon content as principal components. Culture-dependent studies were complementary to culture-independent studies with the majority of actinobacteria isolated not identified in 16S rRNA gene clone libraries. This study demonstrates how the soil physiochemical characteristics and plant species independently determine the community structures of the dominant actinobacteria in distinct ecological habitats. These factors subsequently influence their ecological adaptation, roles and functions. |
format |
Article in Journal/Newspaper |
author |
Sanyika, TW Stafford, William HL Cowan, DA |
author_facet |
Sanyika, TW Stafford, William HL Cowan, DA |
author_sort |
Sanyika, TW |
title |
The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica |
title_short |
The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica |
title_full |
The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica |
title_fullStr |
The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica |
title_full_unstemmed |
The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica |
title_sort |
soil and plant determinants of community structures of the dominant actinobacteria in marion island terrestrial habitats, sub-antarctica |
publisher |
Springer Verlag |
publishDate |
2012 |
url |
http://hdl.handle.net/10204/6697 http://link.springer.com/content/pdf/10.1007/s00300-012-1160-0 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica Marion Island Polar Biology |
genre_facet |
Antarc* Antarctic Antarctica Marion Island Polar Biology |
op_relation |
Workflow;9377 Sanyika, T.W, Stafford, W, Cowan, D.A. 2012. The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica. Polar Biology, vol. 35(8), pp 1129-1141 0722-4060 http://link.springer.com/content/pdf/10.1007/s00300-012-1160-0 http://hdl.handle.net/10204/6697 |
_version_ |
1766071273978331136 |