An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms
Copyright: 2017 Wiley. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website. Autonomous platforms will begin to address the space-time gaps required to improve estimates of...
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ftcsir:oai:researchspace.csir.co.za:10204/10932 2023-05-15T14:01:59+02:00 An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms Thomalla, Sandy J Moutier, William Ryan-Keogh, Thomas J Gregor, Luke Schutt, Julia 2017-12 application/pdf http://hdl.handle.net/10204/10932 http://onlinelibrary.wiley.com/doi/10.1002/lom3.10234/abstract en eng Wiley Worklist;20295 Thomalla, S.J., Moutier, W., Ryan-Keogh, T.J., Gregor, L and Schutt, J. 2017. An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms. Limnology and Oceanography: Methods, DOI:10.1002/lom3.10234 1541-5856 http://onlinelibrary.wiley.com/doi/10.1002/lom3.10234/abstract http://hdl.handle.net/10204/10932 Thomalla, S. J., Moutier, W., Ryan-Keogh, T. J., Gregor, L., & Schutt, J. (2017). An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms. http://hdl.handle.net/10204/10932 Thomalla, Sandy J, William Moutier, Thomas J Ryan-Keogh, Luke Gregor, and Julia Schutt "An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms." (2017) http://hdl.handle.net/10204/10932 Thomalla SJ, Moutier W, Ryan-Keogh TJ, Gregor L, Schutt J. An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms. 2017; http://hdl.handle.net/10204/10932. Sub-Antarctic Southern Ocean Fluorescence quenching Autonomous platforms Backscattering Article 2017 ftcsir https://doi.org/10.1002/lom3.10234 2022-05-19T06:12:09Z Copyright: 2017 Wiley. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website. Autonomous platforms will begin to address the space-time gaps required to improve estimates of phytoplankton distribution, which will aid in the quantification of baseline conditions necessary to detect long-term trends that can be attributed to factors such as climate change. However, there is a need for high quality controlled and verified datasets. In vivo fluorescence provides a proxy for chlorophyll pigment concentration, but it is sensitive to physiological downregulation under incident irradiance (fluorescence quenching). Quenching can undermine the validity of these datasets by underestimating daytime fluorescence derived chlorophyll across regional and temporal scales. Existing methods from the literature have corrected for quenching, however, these methods require certain assumptions to be made that do not hold true across all regions and seasons. The method presented here overcomes some of these assumptions to produce corrected surface fluorescence during the day that closely matched profiles from the previous (or following) night, decreasing the difference to less than 10%. This method corrects daytime quenched fluorescence using a mean nighttime profile of the fluorescence to backscattering ratio multiplied by daytime profiles of backscattering from the surface to the depth of quenching (determined as the depth at which the day fluorescence profile diverges from the mean night profile). This method was applied to a 7-month glider time series in the sub-Antarctic Southern Ocean together with four other methods from the literature for comparison. In addition, the method was applied to a glider time series from the North Atlantic to demonstrate its applicability to other ocean regions. Article in Journal/Newspaper Antarc* Antarctic North Atlantic Southern Ocean Council for Scientific and Industrial Research (South Africa): CSIR Research Space Antarctic Southern Ocean Limnology and Oceanography: Methods 16 2 132 144 |
institution |
Open Polar |
collection |
Council for Scientific and Industrial Research (South Africa): CSIR Research Space |
op_collection_id |
ftcsir |
language |
English |
topic |
Sub-Antarctic Southern Ocean Fluorescence quenching Autonomous platforms Backscattering |
spellingShingle |
Sub-Antarctic Southern Ocean Fluorescence quenching Autonomous platforms Backscattering Thomalla, Sandy J Moutier, William Ryan-Keogh, Thomas J Gregor, Luke Schutt, Julia An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms |
topic_facet |
Sub-Antarctic Southern Ocean Fluorescence quenching Autonomous platforms Backscattering |
description |
Copyright: 2017 Wiley. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website. Autonomous platforms will begin to address the space-time gaps required to improve estimates of phytoplankton distribution, which will aid in the quantification of baseline conditions necessary to detect long-term trends that can be attributed to factors such as climate change. However, there is a need for high quality controlled and verified datasets. In vivo fluorescence provides a proxy for chlorophyll pigment concentration, but it is sensitive to physiological downregulation under incident irradiance (fluorescence quenching). Quenching can undermine the validity of these datasets by underestimating daytime fluorescence derived chlorophyll across regional and temporal scales. Existing methods from the literature have corrected for quenching, however, these methods require certain assumptions to be made that do not hold true across all regions and seasons. The method presented here overcomes some of these assumptions to produce corrected surface fluorescence during the day that closely matched profiles from the previous (or following) night, decreasing the difference to less than 10%. This method corrects daytime quenched fluorescence using a mean nighttime profile of the fluorescence to backscattering ratio multiplied by daytime profiles of backscattering from the surface to the depth of quenching (determined as the depth at which the day fluorescence profile diverges from the mean night profile). This method was applied to a 7-month glider time series in the sub-Antarctic Southern Ocean together with four other methods from the literature for comparison. In addition, the method was applied to a glider time series from the North Atlantic to demonstrate its applicability to other ocean regions. |
format |
Article in Journal/Newspaper |
author |
Thomalla, Sandy J Moutier, William Ryan-Keogh, Thomas J Gregor, Luke Schutt, Julia |
author_facet |
Thomalla, Sandy J Moutier, William Ryan-Keogh, Thomas J Gregor, Luke Schutt, Julia |
author_sort |
Thomalla, Sandy J |
title |
An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms |
title_short |
An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms |
title_full |
An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms |
title_fullStr |
An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms |
title_full_unstemmed |
An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms |
title_sort |
optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms |
publisher |
Wiley |
publishDate |
2017 |
url |
http://hdl.handle.net/10204/10932 http://onlinelibrary.wiley.com/doi/10.1002/lom3.10234/abstract |
geographic |
Antarctic Southern Ocean |
geographic_facet |
Antarctic Southern Ocean |
genre |
Antarc* Antarctic North Atlantic Southern Ocean |
genre_facet |
Antarc* Antarctic North Atlantic Southern Ocean |
op_relation |
Worklist;20295 Thomalla, S.J., Moutier, W., Ryan-Keogh, T.J., Gregor, L and Schutt, J. 2017. An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms. Limnology and Oceanography: Methods, DOI:10.1002/lom3.10234 1541-5856 http://onlinelibrary.wiley.com/doi/10.1002/lom3.10234/abstract http://hdl.handle.net/10204/10932 Thomalla, S. J., Moutier, W., Ryan-Keogh, T. J., Gregor, L., & Schutt, J. (2017). An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms. http://hdl.handle.net/10204/10932 Thomalla, Sandy J, William Moutier, Thomas J Ryan-Keogh, Luke Gregor, and Julia Schutt "An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms." (2017) http://hdl.handle.net/10204/10932 Thomalla SJ, Moutier W, Ryan-Keogh TJ, Gregor L, Schutt J. An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms. 2017; http://hdl.handle.net/10204/10932. |
op_doi |
https://doi.org/10.1002/lom3.10234 |
container_title |
Limnology and Oceanography: Methods |
container_volume |
16 |
container_issue |
2 |
container_start_page |
132 |
op_container_end_page |
144 |
_version_ |
1766272046978826240 |