Use of Multivariate Analysis to Assess the Nutritional Condition of Fish Larvae From Nucleic Acids and Protein Content
11 pages, 3 figures.-- PMID: 12807710 [PubMed]. The nutritional condition of turbot larvae (Scophthalmus maximus) was assessed by a multivariate analysis with DNA, RNA, and protein content as input variables. Special attention was given to the time that feeding began and to the timing and duration o...
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ftcsic:oai:digital.csic.es:10261/9443 2023-05-15T18:15:45+02:00 Use of Multivariate Analysis to Assess the Nutritional Condition of Fish Larvae From Nucleic Acids and Protein Content Cunha, Isabel Saborido-Rey, Fran Planas, Miguel 2003-06 184917 bytes application/pdf http://hdl.handle.net/10261/9443 eng eng Marine Biological Laboratory http://www.biolbull.org/cgi/content/abstract/204/3/339 The Biological Bulletin 204(3): 339-349 (2003) 0006-3185 http://hdl.handle.net/10261/9443 openAccess Turbot larvae Scophthalmus maximus Nutritional condition Multivariate analysis Input variables Developmental stage Artículo 2003 ftcsic 2018-08-21T23:17:44Z 11 pages, 3 figures.-- PMID: 12807710 [PubMed]. The nutritional condition of turbot larvae (Scophthalmus maximus) was assessed by a multivariate analysis with DNA, RNA, and protein content as input variables. Special attention was given to the time that feeding began and to the timing and duration of starvation. The combination of the principal components analysis and the stepwise discriminant analysis, both techniques of multivariate analysis, made it possible to allocate the larvae into groups that were defined and identified based on similarities in developmental stage and nutritional condition. The developmental stage was mostly determined by the input variables DNA and protein content, while nutritional condition was determined by the RNA content. In the period studied, the more developed larvae were less resistant to starvation. Furthermore, when initial feeding was delayed as little as 6 h, the variables analyzed were markedly changed, and the effect on the deprived larvae was found to be equivalent to a 3-day delay in development - when compared with the larvae fed immediately after mouth opening. Through this technique, new samples of larvae with unknown history might be classified into groups, using their DNA, RNA, and protein content as input values in the defined classification functions. Results were compared to those obtained using RNA/DNA and RNA/dry weight indices, and the multivariate method was considered to be more sensitive and to provide extra information about larval nutritional history and development. This study was financially supported by the Comisión Interministerial de Ciencia y Tecnología (CICYT, Spain) under Project AGF 185/92. I. Cunha was supported by a postgraduate scholarship (BD/3489/94) from FCT (Portugal). Peer reviewed Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Turbot larvae Scophthalmus maximus Nutritional condition Multivariate analysis Input variables Developmental stage |
spellingShingle |
Turbot larvae Scophthalmus maximus Nutritional condition Multivariate analysis Input variables Developmental stage Cunha, Isabel Saborido-Rey, Fran Planas, Miguel Use of Multivariate Analysis to Assess the Nutritional Condition of Fish Larvae From Nucleic Acids and Protein Content |
topic_facet |
Turbot larvae Scophthalmus maximus Nutritional condition Multivariate analysis Input variables Developmental stage |
description |
11 pages, 3 figures.-- PMID: 12807710 [PubMed]. The nutritional condition of turbot larvae (Scophthalmus maximus) was assessed by a multivariate analysis with DNA, RNA, and protein content as input variables. Special attention was given to the time that feeding began and to the timing and duration of starvation. The combination of the principal components analysis and the stepwise discriminant analysis, both techniques of multivariate analysis, made it possible to allocate the larvae into groups that were defined and identified based on similarities in developmental stage and nutritional condition. The developmental stage was mostly determined by the input variables DNA and protein content, while nutritional condition was determined by the RNA content. In the period studied, the more developed larvae were less resistant to starvation. Furthermore, when initial feeding was delayed as little as 6 h, the variables analyzed were markedly changed, and the effect on the deprived larvae was found to be equivalent to a 3-day delay in development - when compared with the larvae fed immediately after mouth opening. Through this technique, new samples of larvae with unknown history might be classified into groups, using their DNA, RNA, and protein content as input values in the defined classification functions. Results were compared to those obtained using RNA/DNA and RNA/dry weight indices, and the multivariate method was considered to be more sensitive and to provide extra information about larval nutritional history and development. This study was financially supported by the Comisión Interministerial de Ciencia y Tecnología (CICYT, Spain) under Project AGF 185/92. I. Cunha was supported by a postgraduate scholarship (BD/3489/94) from FCT (Portugal). Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Cunha, Isabel Saborido-Rey, Fran Planas, Miguel |
author_facet |
Cunha, Isabel Saborido-Rey, Fran Planas, Miguel |
author_sort |
Cunha, Isabel |
title |
Use of Multivariate Analysis to Assess the Nutritional Condition of Fish Larvae From Nucleic Acids and Protein Content |
title_short |
Use of Multivariate Analysis to Assess the Nutritional Condition of Fish Larvae From Nucleic Acids and Protein Content |
title_full |
Use of Multivariate Analysis to Assess the Nutritional Condition of Fish Larvae From Nucleic Acids and Protein Content |
title_fullStr |
Use of Multivariate Analysis to Assess the Nutritional Condition of Fish Larvae From Nucleic Acids and Protein Content |
title_full_unstemmed |
Use of Multivariate Analysis to Assess the Nutritional Condition of Fish Larvae From Nucleic Acids and Protein Content |
title_sort |
use of multivariate analysis to assess the nutritional condition of fish larvae from nucleic acids and protein content |
publisher |
Marine Biological Laboratory |
publishDate |
2003 |
url |
http://hdl.handle.net/10261/9443 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://www.biolbull.org/cgi/content/abstract/204/3/339 The Biological Bulletin 204(3): 339-349 (2003) 0006-3185 http://hdl.handle.net/10261/9443 |
op_rights |
openAccess |
_version_ |
1766188950506962944 |