Evaluation of passive samplers as a monitoring tool for early warning of dinophysis toxins in shellfish

• Published in: Marine Drugs
• Main Authors: Pizarro, Gemita, Moroño, Ángeles, Paz, Beatriz, Franco, José M., Pazos, Yolanda, Reguera, B.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Multidisciplinary Digital Publishing Institute 2013
• Subjects: Dinophysis; Early warning DSP outbreaks; HAB monitoring; Modeling toxin accumulation; Solid Phase Adsorbing Toxin Tracking (SPATT); Galician Rías; Patagonia; antartic*
• Online Access: http://hdl.handle.net/10261/88744; https://doi.org/10.3390/md11103823

23 páginas, 9 figuras, 2 tablas From June 2006 to January 2007 passive samplers (solid phase adsorbing toxin tracking, SPATT) were tested as a monitoring tool with weekly monitoring of phytoplankton and toxin content (liquid chromatography–mass spectrometry, LC-MS) in picked cells of Dinophysis and plankton concentrates. Successive blooms of Dinophysis acuminata, D. acuta and D. caudata in 2006 caused a long mussel harvesting closure (4.5 months) in the Galician Rías (NW Spain) and a record (up to 9246 ng·g resin-week−1) accumulation of toxins in SPATT discs. Best fit of a toxin accumulation model was between toxin accumulation in SPATT and the product of cell densities by a constant value, for each species of Dinophysis, of toxin content (average) in picked cells. Detection of Dinophysis populations provided earlier warning of oncoming diarrhetic shellfish poisoning (DSP) outbreaks than the SPATT, which at times overestimated the expected toxin levels in shellfish because: (i) SPATT accumulated toxins did not include biotransformation and depuration loss terms and (ii) accumulation of toxins not available to mussels continued for weeks after Dinophysis cells were undetectable and mussels were toxin-free. SPATT may be a valuable environmental monitoring and research tool for toxin dynamics, in particular in areas with no aquaculture, but does not provide a practical gain for early warning of DSP outbreaks. This research was supported by projects “Dinophysis Galicia” (CTM2004-0478-CO3-01) and ASIMUTH (EU 7FP, SPACE, Grant Agreement # 261860). G. Pizarro was funded by a Chilean pre-doctoral fellowship from the Centro de Estudios del Quaternario Fuego-Patagonia y Antartica (CEQUA) Foundation and the Instituto de Fomento Pesquero (IFOP). This is a contribution to the GEOHAB-Core Research Project HABs in Fjords and Coastal Embayments. Peer reviewed