Nitric oxide synthase type I (nNOS), vascular endothelial growth factor (VEGF) and myoglobin-like expression in skeletal muscle of Antarctic icefishes (Notothenioidei: Channichthyidae)
The Antarctic icefishes Channichthyidae lack haemoglobin and are thought to lack myoglobin (Mb) in their skeletal muscle as well. Due to the absence of both respiratory pigments, icefishes may present a variety of physiological adaptations in their skeletal muscles. In mammals, molecular responses t...
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ftcsic:oai:digital.csic.es:10261/85412 2024-02-11T09:56:54+01:00 Nitric oxide synthase type I (nNOS), vascular endothelial growth factor (VEGF) and myoglobin-like expression in skeletal muscle of Antarctic icefishes (Notothenioidei: Channichthyidae) Morlá, Montse Agustí, Alvar Rahman, Irfan Motterlini, Roberto Saus, Carles Morales-Nin, Beatriz Company, Joan B. Busquets, Xavier 2003 http://hdl.handle.net/10261/85412 en eng Springer issn: 0722-4060 Polar Biology 26: 458- 462 (2003) http://hdl.handle.net/10261/85412 none artículo http://purl.org/coar/resource_type/c_6501 2003 ftcsic 2024-01-16T09:53:32Z The Antarctic icefishes Channichthyidae lack haemoglobin and are thought to lack myoglobin (Mb) in their skeletal muscle as well. Due to the absence of both respiratory pigments, icefishes may present a variety of physiological adaptations in their skeletal muscles. In mammals, molecular responses to limiting oxygen availability in the skeletal muscle include, among others, the over expression of nitric oxide synthases (NOS), such as type I (neuronal nNOS) and type III (endothelial eNOS), as well as the vascular endothelial growth factor (VEGF). In this paper, we evaluated by western blot analysis whether the skeletal muscle of haemoglobin-less icefishes expresses in a constitutive manner higher levels of the type I and type III NOS isoforms and VEGF. Our results demonstrate that haemoglobin-less icefish of the family Channichthyidae do indeed present higher expression of the type I NOS isoform compared with red-blooded Antarctic fish species of other families of the same suborder Notothenioidei. In contrast, VEGF was not over-expressed. Moreover, we show that some icefish species, thought previously to lack Mb in oxidative muscles, actually present Mb-like immunoreactivity in their skeletal muscle. Peer Reviewed Article in Journal/Newspaper Antarc* Antarctic Icefish Polar Biology Digital.CSIC (Spanish National Research Council) Antarctic The Antarctic |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
The Antarctic icefishes Channichthyidae lack haemoglobin and are thought to lack myoglobin (Mb) in their skeletal muscle as well. Due to the absence of both respiratory pigments, icefishes may present a variety of physiological adaptations in their skeletal muscles. In mammals, molecular responses to limiting oxygen availability in the skeletal muscle include, among others, the over expression of nitric oxide synthases (NOS), such as type I (neuronal nNOS) and type III (endothelial eNOS), as well as the vascular endothelial growth factor (VEGF). In this paper, we evaluated by western blot analysis whether the skeletal muscle of haemoglobin-less icefishes expresses in a constitutive manner higher levels of the type I and type III NOS isoforms and VEGF. Our results demonstrate that haemoglobin-less icefish of the family Channichthyidae do indeed present higher expression of the type I NOS isoform compared with red-blooded Antarctic fish species of other families of the same suborder Notothenioidei. In contrast, VEGF was not over-expressed. Moreover, we show that some icefish species, thought previously to lack Mb in oxidative muscles, actually present Mb-like immunoreactivity in their skeletal muscle. Peer Reviewed |
format |
Article in Journal/Newspaper |
author |
Morlá, Montse Agustí, Alvar Rahman, Irfan Motterlini, Roberto Saus, Carles Morales-Nin, Beatriz Company, Joan B. Busquets, Xavier |
spellingShingle |
Morlá, Montse Agustí, Alvar Rahman, Irfan Motterlini, Roberto Saus, Carles Morales-Nin, Beatriz Company, Joan B. Busquets, Xavier Nitric oxide synthase type I (nNOS), vascular endothelial growth factor (VEGF) and myoglobin-like expression in skeletal muscle of Antarctic icefishes (Notothenioidei: Channichthyidae) |
author_facet |
Morlá, Montse Agustí, Alvar Rahman, Irfan Motterlini, Roberto Saus, Carles Morales-Nin, Beatriz Company, Joan B. Busquets, Xavier |
author_sort |
Morlá, Montse |
title |
Nitric oxide synthase type I (nNOS), vascular endothelial growth factor (VEGF) and myoglobin-like expression in skeletal muscle of Antarctic icefishes (Notothenioidei: Channichthyidae) |
title_short |
Nitric oxide synthase type I (nNOS), vascular endothelial growth factor (VEGF) and myoglobin-like expression in skeletal muscle of Antarctic icefishes (Notothenioidei: Channichthyidae) |
title_full |
Nitric oxide synthase type I (nNOS), vascular endothelial growth factor (VEGF) and myoglobin-like expression in skeletal muscle of Antarctic icefishes (Notothenioidei: Channichthyidae) |
title_fullStr |
Nitric oxide synthase type I (nNOS), vascular endothelial growth factor (VEGF) and myoglobin-like expression in skeletal muscle of Antarctic icefishes (Notothenioidei: Channichthyidae) |
title_full_unstemmed |
Nitric oxide synthase type I (nNOS), vascular endothelial growth factor (VEGF) and myoglobin-like expression in skeletal muscle of Antarctic icefishes (Notothenioidei: Channichthyidae) |
title_sort |
nitric oxide synthase type i (nnos), vascular endothelial growth factor (vegf) and myoglobin-like expression in skeletal muscle of antarctic icefishes (notothenioidei: channichthyidae) |
publisher |
Springer |
publishDate |
2003 |
url |
http://hdl.handle.net/10261/85412 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Icefish Polar Biology |
genre_facet |
Antarc* Antarctic Icefish Polar Biology |
op_relation |
issn: 0722-4060 Polar Biology 26: 458- 462 (2003) http://hdl.handle.net/10261/85412 |
op_rights |
none |
_version_ |
1790606848824442880 |