H-HRMAS NMR study of smoked Atlantic salmon (Salmo salar)
High-resolution magic angle spinning (HRMAS) NMR spectroscopic data of smoked Atlantic salmon (Salmo salar) were fully assigned by combination of one- and two-dimensional-HRMAS experiments. Complete representative spectra, obtained after few minutes of analysis time, revealed a large number of minor...
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Online Access: | http://hdl.handle.net/10261/82176 https://doi.org/10.1002/mrc.2652 |
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ftcsic:oai:digital.csic.es:10261/82176 2024-02-11T10:02:07+01:00 H-HRMAS NMR study of smoked Atlantic salmon (Salmo salar) Castejón, David Villa, Palmira Calvo, Marta Santa-María, Guillermo Herraiz Carasa, Marta Herrera, Ana 2010 http://hdl.handle.net/10261/82176 https://doi.org/10.1002/mrc.2652 en eng Wiley-Blackwell doi:10.1002/mrc.2652 issn: 0749-1581 Magnetic Resonance in Chemistry 48: 693- 703 (2010) http://hdl.handle.net/10261/82176 none artículo http://purl.org/coar/resource_type/c_6501 2010 ftcsic https://doi.org/10.1002/mrc.2652 2024-01-16T09:52:05Z High-resolution magic angle spinning (HRMAS) NMR spectroscopic data of smoked Atlantic salmon (Salmo salar) were fully assigned by combination of one- and two-dimensional-HRMAS experiments. Complete representative spectra, obtained after few minutes of analysis time, revealed a large number of minor and major compounds in the sample. The methodology is limited by the low sensitivity of NMR, and therefore HRMAS only enables the determination of the most relevant components. These were fatty acids (FAs), carbohydrates, nucleoside derivatives, osmolytes, amino acids, dipeptides and organic acids. For the first time, spectra were resolved sufficiently to allow semiquantitative determination in intact muscle of the highly polyunsaturated FA 22 : 6 ω-3. Additionally, the feasibility of 1H-HRMAS NMR metabolite profiling was tested to identify some bioactive compounds during storage. This profiling was carried out by the non-destructive and direct analysis (i.e. without requiring sample preparation and multiple step procedures) of intact salmon muscle. The proposed procedure can be applied to a large number of sampleswith high throughput due to the short time of analysis and quick evaluation of the data. Copyright © 2010 John Wiley &Sons, Ltd. Financial support from the Spanish Science and Technology Commission, Project Consolider Ingenio 2010 (CSD2007-00016-03) and the Comunidad de Madrid (Project CM S-0505/AGR-0314) Peer Reviewed Article in Journal/Newspaper Atlantic salmon Salmo salar Digital.CSIC (Spanish National Research Council) Magnetic Resonance in Chemistry 48 9 693 703 |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
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ftcsic |
language |
English |
description |
High-resolution magic angle spinning (HRMAS) NMR spectroscopic data of smoked Atlantic salmon (Salmo salar) were fully assigned by combination of one- and two-dimensional-HRMAS experiments. Complete representative spectra, obtained after few minutes of analysis time, revealed a large number of minor and major compounds in the sample. The methodology is limited by the low sensitivity of NMR, and therefore HRMAS only enables the determination of the most relevant components. These were fatty acids (FAs), carbohydrates, nucleoside derivatives, osmolytes, amino acids, dipeptides and organic acids. For the first time, spectra were resolved sufficiently to allow semiquantitative determination in intact muscle of the highly polyunsaturated FA 22 : 6 ω-3. Additionally, the feasibility of 1H-HRMAS NMR metabolite profiling was tested to identify some bioactive compounds during storage. This profiling was carried out by the non-destructive and direct analysis (i.e. without requiring sample preparation and multiple step procedures) of intact salmon muscle. The proposed procedure can be applied to a large number of sampleswith high throughput due to the short time of analysis and quick evaluation of the data. Copyright © 2010 John Wiley &Sons, Ltd. Financial support from the Spanish Science and Technology Commission, Project Consolider Ingenio 2010 (CSD2007-00016-03) and the Comunidad de Madrid (Project CM S-0505/AGR-0314) Peer Reviewed |
format |
Article in Journal/Newspaper |
author |
Castejón, David Villa, Palmira Calvo, Marta Santa-María, Guillermo Herraiz Carasa, Marta Herrera, Ana |
spellingShingle |
Castejón, David Villa, Palmira Calvo, Marta Santa-María, Guillermo Herraiz Carasa, Marta Herrera, Ana H-HRMAS NMR study of smoked Atlantic salmon (Salmo salar) |
author_facet |
Castejón, David Villa, Palmira Calvo, Marta Santa-María, Guillermo Herraiz Carasa, Marta Herrera, Ana |
author_sort |
Castejón, David |
title |
H-HRMAS NMR study of smoked Atlantic salmon (Salmo salar) |
title_short |
H-HRMAS NMR study of smoked Atlantic salmon (Salmo salar) |
title_full |
H-HRMAS NMR study of smoked Atlantic salmon (Salmo salar) |
title_fullStr |
H-HRMAS NMR study of smoked Atlantic salmon (Salmo salar) |
title_full_unstemmed |
H-HRMAS NMR study of smoked Atlantic salmon (Salmo salar) |
title_sort |
h-hrmas nmr study of smoked atlantic salmon (salmo salar) |
publisher |
Wiley-Blackwell |
publishDate |
2010 |
url |
http://hdl.handle.net/10261/82176 https://doi.org/10.1002/mrc.2652 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.1002/mrc.2652 issn: 0749-1581 Magnetic Resonance in Chemistry 48: 693- 703 (2010) http://hdl.handle.net/10261/82176 |
op_rights |
none |
op_doi |
https://doi.org/10.1002/mrc.2652 |
container_title |
Magnetic Resonance in Chemistry |
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48 |
container_issue |
9 |
container_start_page |
693 |
op_container_end_page |
703 |
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1790598016669843456 |