Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton
Cuvelier, Marie L. . et al.-- 6 pages, 4 figures, 1 table, this article contains supporting information online at https://www.pnas.org/content/pnas/suppl/2010/07/27/1001665107.DCSupplemental/pnas.201001665SI.pdf.-- Data deposition: The sequences reported in this paper have been deposited in the Gen-...
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Online Access: | http://hdl.handle.net/10261/80830 https://doi.org/10.1073/pnas.1001665107 |
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ftcsic:oai:digital.csic.es:10261/80830 2024-02-11T10:06:39+01:00 Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton Cuvelier, Marie L. Latasa, Mikel 2010-08-17 http://hdl.handle.net/10261/80830 https://doi.org/10.1073/pnas.1001665107 en eng National Academy of Sciences (U.S.) https://doi.org/10.1073/pnas.1001665107 doi:10.1073/pnas.1001665107 issn: 0027-8424 e-issn: 1091-6490 Proceedings of the National Academy of Sciences 107: 14679-14684 (2010) http://hdl.handle.net/10261/80830 20668244 none Comparative genomics Primary production Prymnesiophytes Marine photosynthesis Haptophytes artículo http://purl.org/coar/resource_type/c_6501 2010 ftcsic https://doi.org/10.1073/pnas.1001665107 2024-01-16T09:51:31Z Cuvelier, Marie L. . et al.-- 6 pages, 4 figures, 1 table, this article contains supporting information online at https://www.pnas.org/content/pnas/suppl/2010/07/27/1001665107.DCSupplemental/pnas.201001665SI.pdf.-- Data deposition: The sequences reported in this paper have been deposited in the Gen-Bank database (accession nos. HM581528–HM581638 and HM565909–HM565914). Other scaffolds with predicted genes from this Whole Genome Shotgun/454 project have been deposited at DNA Data Bank of Japan/European Molecular Biology Laboratory/GenBank under the accession no. AEAR00000000. The version described in this paper is the first version, AEAR01000000 Among eukaryotes, four major phytoplankton lineages are responsible for marine photosynthesis; prymnesiophytes, alveolates, stramenopiles, and prasinophytes. Contributions by individual taxa, however, are not well known, and genomes have been analyzed fromonly the latter two lineages. Tiny >picoplanktonic> members of the prymnesiophyte lineage have long been inferred to be ecologically important but remain poorly characterized. Here, we examine pico-prymnesiophyte evolutionary history and ecology using cultivation-independent methods. 18S rRNA gene analysis showed picoprymnesiophytes belonged to broadly distributed uncultivated taxa. Therefore, we used targeted metagenomics to analyze uncultured pico-prymnesiophytes sorted by flow cytometry from subtropical North Atlantic waters. The data reveal a composite nuclear-encoded gene repertoire with strong green-lineage affiliations, which contrasts with the evolutionary history indicated by the plastid genome. Measured pico-prymnesiophyte growth rates were rapid in this region, resulting in primary production contributions similar to the cyanobacterium Prochlorococcus. On average, pico-prymnesiophytes formed 25% of global picophytoplankton biomass, with differing contributions in five biogeographical provinces spanning tropical to subpolar systems. Elements likely contributing to success include high gene density and ... Article in Journal/Newspaper North Atlantic Digital.CSIC (Spanish National Research Council) Proceedings of the National Academy of Sciences 107 33 14679 14684 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Comparative genomics Primary production Prymnesiophytes Marine photosynthesis Haptophytes |
spellingShingle |
Comparative genomics Primary production Prymnesiophytes Marine photosynthesis Haptophytes Cuvelier, Marie L. Latasa, Mikel Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
topic_facet |
Comparative genomics Primary production Prymnesiophytes Marine photosynthesis Haptophytes |
description |
Cuvelier, Marie L. . et al.-- 6 pages, 4 figures, 1 table, this article contains supporting information online at https://www.pnas.org/content/pnas/suppl/2010/07/27/1001665107.DCSupplemental/pnas.201001665SI.pdf.-- Data deposition: The sequences reported in this paper have been deposited in the Gen-Bank database (accession nos. HM581528–HM581638 and HM565909–HM565914). Other scaffolds with predicted genes from this Whole Genome Shotgun/454 project have been deposited at DNA Data Bank of Japan/European Molecular Biology Laboratory/GenBank under the accession no. AEAR00000000. The version described in this paper is the first version, AEAR01000000 Among eukaryotes, four major phytoplankton lineages are responsible for marine photosynthesis; prymnesiophytes, alveolates, stramenopiles, and prasinophytes. Contributions by individual taxa, however, are not well known, and genomes have been analyzed fromonly the latter two lineages. Tiny >picoplanktonic> members of the prymnesiophyte lineage have long been inferred to be ecologically important but remain poorly characterized. Here, we examine pico-prymnesiophyte evolutionary history and ecology using cultivation-independent methods. 18S rRNA gene analysis showed picoprymnesiophytes belonged to broadly distributed uncultivated taxa. Therefore, we used targeted metagenomics to analyze uncultured pico-prymnesiophytes sorted by flow cytometry from subtropical North Atlantic waters. The data reveal a composite nuclear-encoded gene repertoire with strong green-lineage affiliations, which contrasts with the evolutionary history indicated by the plastid genome. Measured pico-prymnesiophyte growth rates were rapid in this region, resulting in primary production contributions similar to the cyanobacterium Prochlorococcus. On average, pico-prymnesiophytes formed 25% of global picophytoplankton biomass, with differing contributions in five biogeographical provinces spanning tropical to subpolar systems. Elements likely contributing to success include high gene density and ... |
format |
Article in Journal/Newspaper |
author |
Cuvelier, Marie L. Latasa, Mikel |
author_facet |
Cuvelier, Marie L. Latasa, Mikel |
author_sort |
Cuvelier, Marie L. |
title |
Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
title_short |
Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
title_full |
Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
title_fullStr |
Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
title_full_unstemmed |
Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
title_sort |
targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
publisher |
National Academy of Sciences (U.S.) |
publishDate |
2010 |
url |
http://hdl.handle.net/10261/80830 https://doi.org/10.1073/pnas.1001665107 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
https://doi.org/10.1073/pnas.1001665107 doi:10.1073/pnas.1001665107 issn: 0027-8424 e-issn: 1091-6490 Proceedings of the National Academy of Sciences 107: 14679-14684 (2010) http://hdl.handle.net/10261/80830 20668244 |
op_rights |
none |
op_doi |
https://doi.org/10.1073/pnas.1001665107 |
container_title |
Proceedings of the National Academy of Sciences |
container_volume |
107 |
container_issue |
33 |
container_start_page |
14679 |
op_container_end_page |
14684 |
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1790604476342599680 |