Protection and antibody response induced by intramuscular DNA vaccine encoding for viral haemorrhagic septicaemia virus (VHSV) G glycoprotein in turbot (Scophthalmus maximus)
7 páginas, 5 figuras Turbot (Scophthalmus maximus) is a high-value farmed marine flatfish with growing demand and production levels in Europe susceptible to turbot-specific viral haemorrhagic septicaemia virus (VHSV) strains. To evaluate the possibility of controlling the outbreaks of this infectiou...
Published in: | Fish & Shellfish Immunology |
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Online Access: | http://hdl.handle.net/10261/64702 https://doi.org/10.1016/j.fsi.2012.03.004 |
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ftcsic:oai:digital.csic.es:10261/64702 2024-02-11T10:08:26+01:00 Protection and antibody response induced by intramuscular DNA vaccine encoding for viral haemorrhagic septicaemia virus (VHSV) G glycoprotein in turbot (Scophthalmus maximus) Pereiro, Patricia Martínez-López, Alicia Falcó, Alberto Dios, S. Figueras Huerta, Antonio Coll, Julio Novoa, Beatriz Estepa, Amparo 2012 http://hdl.handle.net/10261/64702 https://doi.org/10.1016/j.fsi.2012.03.004 en eng Elsevier http://dx.doi.org/10.1016/j.fsi.2012.03.004 Fish and Shellfish Immunology 32(6): 1088-1096 (2012) 1050-4648 http://hdl.handle.net/10261/64702 doi:10.1016/j.fsi.2012.03.004 1095-9947 none DNA vaccine VHSV Turbot Glycoprotein G Rhabdovirus artículo http://purl.org/coar/resource_type/c_6501 2012 ftcsic https://doi.org/10.1016/j.fsi.2012.03.004 2024-01-16T09:44:30Z 7 páginas, 5 figuras Turbot (Scophthalmus maximus) is a high-value farmed marine flatfish with growing demand and production levels in Europe susceptible to turbot-specific viral haemorrhagic septicaemia virus (VHSV) strains. To evaluate the possibility of controlling the outbreaks of this infectious disease by means of DNA vaccination, the gpG of a VHSV isolated from farmed turbot (VHSV860) was cloned into an expression plasmid containing the human cytomegalovirus (CMV) promoter (pMCV1.4-G860). In our experimental conditions, DNA immunised turbots were more than 85% protected against VHSV860 lethal challenge and showed both VHSV-gpG specific and neutralizing antibodies. To our knowledge this is the first report showing the efficacy of turbot genetic immunisation against a VHSV. Work is in progress to determine the contribution of innate and adaptive immunity to the protective response elicited by the immunization. This work was supported by the projects Consolider Ingenio 2010, CSD2007-00002 and AGL2011-28921-C03 both from the Spanish Ministerio de Ciencia e Innovación (MICINN). Peer reviewed Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Fish & Shellfish Immunology 32 6 1088 1094 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
DNA vaccine VHSV Turbot Glycoprotein G Rhabdovirus |
spellingShingle |
DNA vaccine VHSV Turbot Glycoprotein G Rhabdovirus Pereiro, Patricia Martínez-López, Alicia Falcó, Alberto Dios, S. Figueras Huerta, Antonio Coll, Julio Novoa, Beatriz Estepa, Amparo Protection and antibody response induced by intramuscular DNA vaccine encoding for viral haemorrhagic septicaemia virus (VHSV) G glycoprotein in turbot (Scophthalmus maximus) |
topic_facet |
DNA vaccine VHSV Turbot Glycoprotein G Rhabdovirus |
description |
7 páginas, 5 figuras Turbot (Scophthalmus maximus) is a high-value farmed marine flatfish with growing demand and production levels in Europe susceptible to turbot-specific viral haemorrhagic septicaemia virus (VHSV) strains. To evaluate the possibility of controlling the outbreaks of this infectious disease by means of DNA vaccination, the gpG of a VHSV isolated from farmed turbot (VHSV860) was cloned into an expression plasmid containing the human cytomegalovirus (CMV) promoter (pMCV1.4-G860). In our experimental conditions, DNA immunised turbots were more than 85% protected against VHSV860 lethal challenge and showed both VHSV-gpG specific and neutralizing antibodies. To our knowledge this is the first report showing the efficacy of turbot genetic immunisation against a VHSV. Work is in progress to determine the contribution of innate and adaptive immunity to the protective response elicited by the immunization. This work was supported by the projects Consolider Ingenio 2010, CSD2007-00002 and AGL2011-28921-C03 both from the Spanish Ministerio de Ciencia e Innovación (MICINN). Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Pereiro, Patricia Martínez-López, Alicia Falcó, Alberto Dios, S. Figueras Huerta, Antonio Coll, Julio Novoa, Beatriz Estepa, Amparo |
author_facet |
Pereiro, Patricia Martínez-López, Alicia Falcó, Alberto Dios, S. Figueras Huerta, Antonio Coll, Julio Novoa, Beatriz Estepa, Amparo |
author_sort |
Pereiro, Patricia |
title |
Protection and antibody response induced by intramuscular DNA vaccine encoding for viral haemorrhagic septicaemia virus (VHSV) G glycoprotein in turbot (Scophthalmus maximus) |
title_short |
Protection and antibody response induced by intramuscular DNA vaccine encoding for viral haemorrhagic septicaemia virus (VHSV) G glycoprotein in turbot (Scophthalmus maximus) |
title_full |
Protection and antibody response induced by intramuscular DNA vaccine encoding for viral haemorrhagic septicaemia virus (VHSV) G glycoprotein in turbot (Scophthalmus maximus) |
title_fullStr |
Protection and antibody response induced by intramuscular DNA vaccine encoding for viral haemorrhagic septicaemia virus (VHSV) G glycoprotein in turbot (Scophthalmus maximus) |
title_full_unstemmed |
Protection and antibody response induced by intramuscular DNA vaccine encoding for viral haemorrhagic septicaemia virus (VHSV) G glycoprotein in turbot (Scophthalmus maximus) |
title_sort |
protection and antibody response induced by intramuscular dna vaccine encoding for viral haemorrhagic septicaemia virus (vhsv) g glycoprotein in turbot (scophthalmus maximus) |
publisher |
Elsevier |
publishDate |
2012 |
url |
http://hdl.handle.net/10261/64702 https://doi.org/10.1016/j.fsi.2012.03.004 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://dx.doi.org/10.1016/j.fsi.2012.03.004 Fish and Shellfish Immunology 32(6): 1088-1096 (2012) 1050-4648 http://hdl.handle.net/10261/64702 doi:10.1016/j.fsi.2012.03.004 1095-9947 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.fsi.2012.03.004 |
container_title |
Fish & Shellfish Immunology |
container_volume |
32 |
container_issue |
6 |
container_start_page |
1088 |
op_container_end_page |
1094 |
_version_ |
1790607750802178048 |