Sequential injection analysis for automation of the Winkler methodology, with real-time SIMPLEX optimization and shipboard application
A multipurpose analyzer system based on sequential injection analysis (SIA) for the determination of dissolved oxygen (DO) in seawater is presented. Three operation modes were established and successfully applied onboard during a research cruise in the Southern ocean: 1st, in-line execution of the e...
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ftcsic:oai:digital.csic.es:10261/60324 2024-02-11T10:08:53+01:00 Sequential injection analysis for automation of the Winkler methodology, with real-time SIMPLEX optimization and shipboard application Horstkotte, Burkhard Tovar-Sánchez, Antonio Duarte, Carlos M. Cerdà, Víctor 2010 http://hdl.handle.net/10261/60324 https://doi.org/10.1016/j.aca.2009.11.018 en eng Elsevier doi:10.1016/j.aca.2009.11.018 issn: 0003-2670 Analytica Chimica Acta 658: 147- 155 (2010) http://hdl.handle.net/10261/60324 open artículo http://purl.org/coar/resource_type/c_6501 2010 ftcsic https://doi.org/10.1016/j.aca.2009.11.018 2024-01-16T09:42:31Z A multipurpose analyzer system based on sequential injection analysis (SIA) for the determination of dissolved oxygen (DO) in seawater is presented. Three operation modes were established and successfully applied onboard during a research cruise in the Southern ocean: 1st, in-line execution of the entire Winkler method including precipitation of manganese (II) hydroxide, fixation of DO, precipitate dissolution by confluent acidification, and spectrophotometric quantification of the generated iodine/tri-iodide (I2/I3 -), 2nd, spectrophotometric quantification of I2/I3 - in samples prepared according the classical Winkler protocol, and 3rd, accurate batch-wise titration of I2/I3 - with thiosulfate using one syringe pump of the analyzer as automatic burette. In the first mode, the zone stacking principle was applied to achieve high dispersion of the reagent solutions in the sample zone. Spectrophotometric detection was done at the isobestic wavelength 466 nm of I2/I3 -. Highly reduced consumption of reagents and sample compared to the classical Winkler protocol, linear response up to 16 mg L-1 DO, and an injection frequency of 30 per hour were achieved. It is noteworthy that for the offline protocol, sample metering and quantification with a potentiometric titrator lasts in general over 5 min without counting sample fixation, incubation, and glassware cleaning. The modified SIMPLEX methodology was used for the simultaneous optimization of four volumetric and two chemical variables. Vertex calculation and consequent application including in-line preparation of one reagent was carried out in real-time using the software AutoAnalysis. The analytical system featured high signal stability, robustness, and a repeatability of 3% RSD (1st mode) and 0.8% (2nd mode) during shipboard application. © 2009 Elsevier B.V. All rights reserved. This work was funded by the projects “Design and application of analytical systems for the quantification of trace elements in the ocean” supported from the Government of the Balearic ... Article in Journal/Newspaper Southern Ocean Digital.CSIC (Spanish National Research Council) Southern Ocean Analytica Chimica Acta 658 2 147 155 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
A multipurpose analyzer system based on sequential injection analysis (SIA) for the determination of dissolved oxygen (DO) in seawater is presented. Three operation modes were established and successfully applied onboard during a research cruise in the Southern ocean: 1st, in-line execution of the entire Winkler method including precipitation of manganese (II) hydroxide, fixation of DO, precipitate dissolution by confluent acidification, and spectrophotometric quantification of the generated iodine/tri-iodide (I2/I3 -), 2nd, spectrophotometric quantification of I2/I3 - in samples prepared according the classical Winkler protocol, and 3rd, accurate batch-wise titration of I2/I3 - with thiosulfate using one syringe pump of the analyzer as automatic burette. In the first mode, the zone stacking principle was applied to achieve high dispersion of the reagent solutions in the sample zone. Spectrophotometric detection was done at the isobestic wavelength 466 nm of I2/I3 -. Highly reduced consumption of reagents and sample compared to the classical Winkler protocol, linear response up to 16 mg L-1 DO, and an injection frequency of 30 per hour were achieved. It is noteworthy that for the offline protocol, sample metering and quantification with a potentiometric titrator lasts in general over 5 min without counting sample fixation, incubation, and glassware cleaning. The modified SIMPLEX methodology was used for the simultaneous optimization of four volumetric and two chemical variables. Vertex calculation and consequent application including in-line preparation of one reagent was carried out in real-time using the software AutoAnalysis. The analytical system featured high signal stability, robustness, and a repeatability of 3% RSD (1st mode) and 0.8% (2nd mode) during shipboard application. © 2009 Elsevier B.V. All rights reserved. This work was funded by the projects “Design and application of analytical systems for the quantification of trace elements in the ocean” supported from the Government of the Balearic ... |
format |
Article in Journal/Newspaper |
author |
Horstkotte, Burkhard Tovar-Sánchez, Antonio Duarte, Carlos M. Cerdà, Víctor |
spellingShingle |
Horstkotte, Burkhard Tovar-Sánchez, Antonio Duarte, Carlos M. Cerdà, Víctor Sequential injection analysis for automation of the Winkler methodology, with real-time SIMPLEX optimization and shipboard application |
author_facet |
Horstkotte, Burkhard Tovar-Sánchez, Antonio Duarte, Carlos M. Cerdà, Víctor |
author_sort |
Horstkotte, Burkhard |
title |
Sequential injection analysis for automation of the Winkler methodology, with real-time SIMPLEX optimization and shipboard application |
title_short |
Sequential injection analysis for automation of the Winkler methodology, with real-time SIMPLEX optimization and shipboard application |
title_full |
Sequential injection analysis for automation of the Winkler methodology, with real-time SIMPLEX optimization and shipboard application |
title_fullStr |
Sequential injection analysis for automation of the Winkler methodology, with real-time SIMPLEX optimization and shipboard application |
title_full_unstemmed |
Sequential injection analysis for automation of the Winkler methodology, with real-time SIMPLEX optimization and shipboard application |
title_sort |
sequential injection analysis for automation of the winkler methodology, with real-time simplex optimization and shipboard application |
publisher |
Elsevier |
publishDate |
2010 |
url |
http://hdl.handle.net/10261/60324 https://doi.org/10.1016/j.aca.2009.11.018 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_relation |
doi:10.1016/j.aca.2009.11.018 issn: 0003-2670 Analytica Chimica Acta 658: 147- 155 (2010) http://hdl.handle.net/10261/60324 |
op_rights |
open |
op_doi |
https://doi.org/10.1016/j.aca.2009.11.018 |
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Analytica Chimica Acta |
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658 |
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2 |
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147 |
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155 |
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1790608519843545088 |