Kinetics of phagocytic activity, plaque-forming cells and specific agglutinins of turbot (Scophthalmus maximus L.) immunised with O antigen of Vibrio damsela and Pasteurella piscicida

• Published in: Fish & Shellfish Immunology
• Main Authors: Santarém, M. M., Figueras Huerta, Antonio
• Format: Article in Journal/Newspaper
• Language: English
• Published: Academic Press 1994
• Subjects: Bacterins; Vibrio damsela; Pasteurella piscicida; Phagocytosis; Plaque-forming celis; Scophthalmus maximus; Turbot
• Online Access: http://hdl.handle.net/10261/60077; https://doi.org/10.1006/fsim.1994.1047

11 páginas, 1 tabla, 5 figuras The O antigens derived from Vibrio damsela and Pasteurella piscicida were evaluated to determine their ability to enhance non-specific defence mechanisms and the specific immune response in turbot. Intraperitoneal injections of 100 μg V. damsela or P. piscicida O antigen resulted in an increased phagocytic activity of splenic leucocytes 3 days after immunisation, although the level of phagocytosis of sheep red blood cells (SRBC) was always low. In both groups of immunised fish a peak in the specific plaque-forming cells (PFC) was observed 21 days post-injection. There were no differences in PFC number induced by the two bacterins in turbot. Peak antibody titres were 28 and 42 days after immunisation with O antigen from P. piscicida and V. damsela respectively. CICYT MAR91-1133-CO2-02 Peer reviewed