Sunlight Effects on the Osmotrophic Uptake of DMSP-Sulfur and Leucine by Polar Phytoplankton
14 pages, 8 figures, 4 tables Even though the uptake and assimilation of organic compounds by phytoplankton has been long recognized, very little is still known about its potential ecological role in natural marine communities and whether it varies depending on the light regimes the algae experience...
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ftcsic:oai:digital.csic.es:10261/59885 2024-02-11T09:57:19+01:00 Sunlight Effects on the Osmotrophic Uptake of DMSP-Sulfur and Leucine by Polar Phytoplankton Ruiz-González, Clara Galí, Martí Sintes, Eva Herndl, Gerhard J. Gasol, Josep M. Simó, Rafel 2012-09-19 http://hdl.handle.net/10261/59885 https://doi.org/10.1371/journal.pone.0045545 en eng Public Library of Science Publisher’s version https://doi.org/10.1371/journal.pone.0045545 PLoS ONE 7(9): e45545 1932-6203 http://hdl.handle.net/10261/59885 doi:10.1371/journal.pone.0045545 23029084 open artículo http://purl.org/coar/resource_type/c_6501 2012 ftcsic https://doi.org/10.1371/journal.pone.0045545 2024-01-16T09:42:20Z 14 pages, 8 figures, 4 tables Even though the uptake and assimilation of organic compounds by phytoplankton has been long recognized, very little is still known about its potential ecological role in natural marine communities and whether it varies depending on the light regimes the algae experience. We combined measurements of size-fractionated assimilation of trace additions of 3H-leucine and 35S-dimethylsulfoniopropionate (DMSP) with microautoradiography to assess the extent and relevance of osmoheterotrophy in summer phytoplankton assemblages from Arctic and Antarctic waters, and the role of solar radiation on it was further investigated by exposing samples to different radiation spectra. Significant assimilation of both substrates occurred in the size fraction containing most phytoplankton (>5 µm), sunlight exposure generally increasing 35S-DMSP-sulfur assimilation and decreasing 3H-leucine assimilation. Microautoradiography revealed that the capacity to take up both organic substrates seemed widespread among different polar algal phyla, particularly in pennate and centric diatoms, and photosynthetic dinoflagellates. Image analysis of the microautoradiograms showed for the first time interspecific variability in the uptakes of 35S-DMSP and 3H-leucine by phytoplankton depending on the solar spectrum. Overall, these results suggest that the role of polar phytoplankton in the utilization of labile dissolved organic matter may be significant under certain conditions and further confirm the relevance of solar radiation in regulating heterotrophy in the pelagic ocean This study was supported by the Spanish Ministry of Science and Innovation through a PhD studentship to Clara Ruiz-González and through the projects ATOS (POL2006-00550/CTM), MODIVUS (CTM2005-04795/MAR) and SUMMER (CTM2008-03309/MAR). The funders only provided funding after recommendation by a peer review panel. They had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript Peer reviewed Article in Journal/Newspaper Antarc* Antarctic Arctic Phytoplankton Digital.CSIC (Spanish National Research Council) Antarctic Arctic PLoS ONE 7 9 e45545 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
14 pages, 8 figures, 4 tables Even though the uptake and assimilation of organic compounds by phytoplankton has been long recognized, very little is still known about its potential ecological role in natural marine communities and whether it varies depending on the light regimes the algae experience. We combined measurements of size-fractionated assimilation of trace additions of 3H-leucine and 35S-dimethylsulfoniopropionate (DMSP) with microautoradiography to assess the extent and relevance of osmoheterotrophy in summer phytoplankton assemblages from Arctic and Antarctic waters, and the role of solar radiation on it was further investigated by exposing samples to different radiation spectra. Significant assimilation of both substrates occurred in the size fraction containing most phytoplankton (>5 µm), sunlight exposure generally increasing 35S-DMSP-sulfur assimilation and decreasing 3H-leucine assimilation. Microautoradiography revealed that the capacity to take up both organic substrates seemed widespread among different polar algal phyla, particularly in pennate and centric diatoms, and photosynthetic dinoflagellates. Image analysis of the microautoradiograms showed for the first time interspecific variability in the uptakes of 35S-DMSP and 3H-leucine by phytoplankton depending on the solar spectrum. Overall, these results suggest that the role of polar phytoplankton in the utilization of labile dissolved organic matter may be significant under certain conditions and further confirm the relevance of solar radiation in regulating heterotrophy in the pelagic ocean This study was supported by the Spanish Ministry of Science and Innovation through a PhD studentship to Clara Ruiz-González and through the projects ATOS (POL2006-00550/CTM), MODIVUS (CTM2005-04795/MAR) and SUMMER (CTM2008-03309/MAR). The funders only provided funding after recommendation by a peer review panel. They had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Ruiz-González, Clara Galí, Martí Sintes, Eva Herndl, Gerhard J. Gasol, Josep M. Simó, Rafel |
spellingShingle |
Ruiz-González, Clara Galí, Martí Sintes, Eva Herndl, Gerhard J. Gasol, Josep M. Simó, Rafel Sunlight Effects on the Osmotrophic Uptake of DMSP-Sulfur and Leucine by Polar Phytoplankton |
author_facet |
Ruiz-González, Clara Galí, Martí Sintes, Eva Herndl, Gerhard J. Gasol, Josep M. Simó, Rafel |
author_sort |
Ruiz-González, Clara |
title |
Sunlight Effects on the Osmotrophic Uptake of DMSP-Sulfur and Leucine by Polar Phytoplankton |
title_short |
Sunlight Effects on the Osmotrophic Uptake of DMSP-Sulfur and Leucine by Polar Phytoplankton |
title_full |
Sunlight Effects on the Osmotrophic Uptake of DMSP-Sulfur and Leucine by Polar Phytoplankton |
title_fullStr |
Sunlight Effects on the Osmotrophic Uptake of DMSP-Sulfur and Leucine by Polar Phytoplankton |
title_full_unstemmed |
Sunlight Effects on the Osmotrophic Uptake of DMSP-Sulfur and Leucine by Polar Phytoplankton |
title_sort |
sunlight effects on the osmotrophic uptake of dmsp-sulfur and leucine by polar phytoplankton |
publisher |
Public Library of Science |
publishDate |
2012 |
url |
http://hdl.handle.net/10261/59885 https://doi.org/10.1371/journal.pone.0045545 |
geographic |
Antarctic Arctic |
geographic_facet |
Antarctic Arctic |
genre |
Antarc* Antarctic Arctic Phytoplankton |
genre_facet |
Antarc* Antarctic Arctic Phytoplankton |
op_relation |
Publisher’s version https://doi.org/10.1371/journal.pone.0045545 PLoS ONE 7(9): e45545 1932-6203 http://hdl.handle.net/10261/59885 doi:10.1371/journal.pone.0045545 23029084 |
op_rights |
open |
op_doi |
https://doi.org/10.1371/journal.pone.0045545 |
container_title |
PLoS ONE |
container_volume |
7 |
container_issue |
9 |
container_start_page |
e45545 |
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1790609610947690496 |