Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot, Scophthalmus maximus
12 páginas, 8 figuras, 3 tablas This report describes the cloning, characterization and gene expression pattern of two Th17 cytokines, interleukin (IL)-17A/F and -22, in turbot Scophthalmus maximus. The turbot IL-17A/F cDNA contains a 516 bp open reading frame encoding a deduced IL-17A/F protein of...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://hdl.handle.net/10261/59774 https://doi.org/10.1016/j.dci.2012.09.002 |
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ftcsic:oai:digital.csic.es:10261/59774 2024-02-11T10:08:26+01:00 Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot, Scophthalmus maximus Costa, M. M. Pereiro, Patricia Wang, T. Secombes, Christopher J. Figueras Huerta, Antonio Novoa, Beatriz 2012 http://hdl.handle.net/10261/59774 https://doi.org/10.1016/j.dci.2012.09.002 en eng Elsevier http://dx.doi.org/10.1016/j.dci.2012.09.002 Developmental and Comparative Immunology 38(4): 505-516 (2012) 0145-305X http://hdl.handle.net/10261/59774 doi:10.1016/j.dci.2012.09.002 1879-0089 open IL-22 IL-17 Th-17 response Mucosal-associated immunity artículo http://purl.org/coar/resource_type/c_6501 2012 ftcsic https://doi.org/10.1016/j.dci.2012.09.002 2024-01-16T09:42:17Z 12 páginas, 8 figuras, 3 tablas This report describes the cloning, characterization and gene expression pattern of two Th17 cytokines, interleukin (IL)-17A/F and -22, in turbot Scophthalmus maximus. The turbot IL-17A/F cDNA contains a 516 bp open reading frame encoding a deduced IL-17A/F protein of 171 amino acid (aa) residues, containing a predicted signal peptide of 31 aa. Turbot IL-22 had a 564 bp ORF coding for a 187 aa protein with a 33 aa signal peptide. The turbot IL-22 protein contained a typical IL-10 family signature. Both cytokines had highest expression levels in the intestine followed by head kidney and gills. Stimulation with the Gram negative bacterium Aeromonas salmonicida was able to modulate IL-17A/F and IL-22 expression in head kidney, spleen and liver but not the intestine. PMA and PHA were also able to induce the expression of both cytokines, suggesting that, as expected, T-cells are likely the main producers of these molecules in turbot as in mammals. This work was supported by the projects CSD2007-00002 ‘‘Aquagenomics’’ of the program Consolider-Ingenio 2010 and AGL2009- 10677 from the Spanish Ministerio de Ciencia e Innovación. Peer reviewed Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Developmental & Comparative Immunology 38 4 505 516 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
IL-22 IL-17 Th-17 response Mucosal-associated immunity |
spellingShingle |
IL-22 IL-17 Th-17 response Mucosal-associated immunity Costa, M. M. Pereiro, Patricia Wang, T. Secombes, Christopher J. Figueras Huerta, Antonio Novoa, Beatriz Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot, Scophthalmus maximus |
topic_facet |
IL-22 IL-17 Th-17 response Mucosal-associated immunity |
description |
12 páginas, 8 figuras, 3 tablas This report describes the cloning, characterization and gene expression pattern of two Th17 cytokines, interleukin (IL)-17A/F and -22, in turbot Scophthalmus maximus. The turbot IL-17A/F cDNA contains a 516 bp open reading frame encoding a deduced IL-17A/F protein of 171 amino acid (aa) residues, containing a predicted signal peptide of 31 aa. Turbot IL-22 had a 564 bp ORF coding for a 187 aa protein with a 33 aa signal peptide. The turbot IL-22 protein contained a typical IL-10 family signature. Both cytokines had highest expression levels in the intestine followed by head kidney and gills. Stimulation with the Gram negative bacterium Aeromonas salmonicida was able to modulate IL-17A/F and IL-22 expression in head kidney, spleen and liver but not the intestine. PMA and PHA were also able to induce the expression of both cytokines, suggesting that, as expected, T-cells are likely the main producers of these molecules in turbot as in mammals. This work was supported by the projects CSD2007-00002 ‘‘Aquagenomics’’ of the program Consolider-Ingenio 2010 and AGL2009- 10677 from the Spanish Ministerio de Ciencia e Innovación. Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Costa, M. M. Pereiro, Patricia Wang, T. Secombes, Christopher J. Figueras Huerta, Antonio Novoa, Beatriz |
author_facet |
Costa, M. M. Pereiro, Patricia Wang, T. Secombes, Christopher J. Figueras Huerta, Antonio Novoa, Beatriz |
author_sort |
Costa, M. M. |
title |
Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot, Scophthalmus maximus |
title_short |
Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot, Scophthalmus maximus |
title_full |
Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot, Scophthalmus maximus |
title_fullStr |
Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot, Scophthalmus maximus |
title_full_unstemmed |
Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot, Scophthalmus maximus |
title_sort |
characterization and gene expression analysis of the two main th17 cytokines (il-17a/f and il-22) in turbot, scophthalmus maximus |
publisher |
Elsevier |
publishDate |
2012 |
url |
http://hdl.handle.net/10261/59774 https://doi.org/10.1016/j.dci.2012.09.002 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://dx.doi.org/10.1016/j.dci.2012.09.002 Developmental and Comparative Immunology 38(4): 505-516 (2012) 0145-305X http://hdl.handle.net/10261/59774 doi:10.1016/j.dci.2012.09.002 1879-0089 |
op_rights |
open |
op_doi |
https://doi.org/10.1016/j.dci.2012.09.002 |
container_title |
Developmental & Comparative Immunology |
container_volume |
38 |
container_issue |
4 |
container_start_page |
505 |
op_container_end_page |
516 |
_version_ |
1790607758500823040 |