Isolation and characterization of nine polymorphic microsatellite markers in the two-banded sea bream (Diplodus vulgaris) and cross-species amplification in the white sea bream (Diplodus sargus) and the saddled bream (Oblada melanura)
We have developed nine new microsatellite markers for the two-banded sea bream (Diplodus vulgaris) from an enriched genome library protocol. All these loci are polymorphic, with mean allelic diversity of 13 (range 5 -21), and expected and observed heterozygosities from 0.641 to 0.932 and 0.428 to 0....
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Online Access: | http://hdl.handle.net/10261/59428 https://doi.org/10.1111/j.1471-8286.2006.01667.x |
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ftcsic:oai:digital.csic.es:10261/59428 2024-02-11T10:09:23+01:00 Isolation and characterization of nine polymorphic microsatellite markers in the two-banded sea bream (Diplodus vulgaris) and cross-species amplification in the white sea bream (Diplodus sargus) and the saddled bream (Oblada melanura) Roques, Séverine Galarza, Juan A. Macpherson, Enrique Turner, George F. Rico, Ciro 2007 http://hdl.handle.net/10261/59428 https://doi.org/10.1111/j.1471-8286.2006.01667.x en eng Blackwell Publishing doi:10.1111/j.1471-8286.2006.01667.x issn: 1471-8278 MOLECULAR ECOLOGY NOTES 7: 661- 663 (2007) http://hdl.handle.net/10261/59428 open artículo http://purl.org/coar/resource_type/c_6501 2007 ftcsic https://doi.org/10.1111/j.1471-8286.2006.01667.x 2024-01-16T09:42:07Z We have developed nine new microsatellite markers for the two-banded sea bream (Diplodus vulgaris) from an enriched genome library protocol. All these loci are polymorphic, with mean allelic diversity of 13 (range 5 -21), and expected and observed heterozygosities from 0.641 to 0.932 and 0.428 to 0.914, respectively. Cross-species tests in two close-related species of the genus Diplodus (D. sargus and O. melanura) revealed successful amplifications at 8 out of 9 loci, with mean allele number of 4.75 (range 2-8) and 5.50 (range 3 -10), respectively. These results are consistent with the close phylogenetic relationships between the three species, indicating this set of primers might prove useful for studying the levels of genetic diversity and population differentiation in these three species and in other phylogenetically close species of the genus Diplodus and Sparus. © 2006 The Authors. Peer Reviewed Article in Journal/Newspaper White Sea Digital.CSIC (Spanish National Research Council) White Sea Molecular Ecology Notes 7 4 661 663 |
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Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
We have developed nine new microsatellite markers for the two-banded sea bream (Diplodus vulgaris) from an enriched genome library protocol. All these loci are polymorphic, with mean allelic diversity of 13 (range 5 -21), and expected and observed heterozygosities from 0.641 to 0.932 and 0.428 to 0.914, respectively. Cross-species tests in two close-related species of the genus Diplodus (D. sargus and O. melanura) revealed successful amplifications at 8 out of 9 loci, with mean allele number of 4.75 (range 2-8) and 5.50 (range 3 -10), respectively. These results are consistent with the close phylogenetic relationships between the three species, indicating this set of primers might prove useful for studying the levels of genetic diversity and population differentiation in these three species and in other phylogenetically close species of the genus Diplodus and Sparus. © 2006 The Authors. Peer Reviewed |
format |
Article in Journal/Newspaper |
author |
Roques, Séverine Galarza, Juan A. Macpherson, Enrique Turner, George F. Rico, Ciro |
spellingShingle |
Roques, Séverine Galarza, Juan A. Macpherson, Enrique Turner, George F. Rico, Ciro Isolation and characterization of nine polymorphic microsatellite markers in the two-banded sea bream (Diplodus vulgaris) and cross-species amplification in the white sea bream (Diplodus sargus) and the saddled bream (Oblada melanura) |
author_facet |
Roques, Séverine Galarza, Juan A. Macpherson, Enrique Turner, George F. Rico, Ciro |
author_sort |
Roques, Séverine |
title |
Isolation and characterization of nine polymorphic microsatellite markers in the two-banded sea bream (Diplodus vulgaris) and cross-species amplification in the white sea bream (Diplodus sargus) and the saddled bream (Oblada melanura) |
title_short |
Isolation and characterization of nine polymorphic microsatellite markers in the two-banded sea bream (Diplodus vulgaris) and cross-species amplification in the white sea bream (Diplodus sargus) and the saddled bream (Oblada melanura) |
title_full |
Isolation and characterization of nine polymorphic microsatellite markers in the two-banded sea bream (Diplodus vulgaris) and cross-species amplification in the white sea bream (Diplodus sargus) and the saddled bream (Oblada melanura) |
title_fullStr |
Isolation and characterization of nine polymorphic microsatellite markers in the two-banded sea bream (Diplodus vulgaris) and cross-species amplification in the white sea bream (Diplodus sargus) and the saddled bream (Oblada melanura) |
title_full_unstemmed |
Isolation and characterization of nine polymorphic microsatellite markers in the two-banded sea bream (Diplodus vulgaris) and cross-species amplification in the white sea bream (Diplodus sargus) and the saddled bream (Oblada melanura) |
title_sort |
isolation and characterization of nine polymorphic microsatellite markers in the two-banded sea bream (diplodus vulgaris) and cross-species amplification in the white sea bream (diplodus sargus) and the saddled bream (oblada melanura) |
publisher |
Blackwell Publishing |
publishDate |
2007 |
url |
http://hdl.handle.net/10261/59428 https://doi.org/10.1111/j.1471-8286.2006.01667.x |
geographic |
White Sea |
geographic_facet |
White Sea |
genre |
White Sea |
genre_facet |
White Sea |
op_relation |
doi:10.1111/j.1471-8286.2006.01667.x issn: 1471-8278 MOLECULAR ECOLOGY NOTES 7: 661- 663 (2007) http://hdl.handle.net/10261/59428 |
op_rights |
open |
op_doi |
https://doi.org/10.1111/j.1471-8286.2006.01667.x |
container_title |
Molecular Ecology Notes |
container_volume |
7 |
container_issue |
4 |
container_start_page |
661 |
op_container_end_page |
663 |
_version_ |
1790609276864036864 |