Production of leukotriene B4 and prostaglandin E2 by turbot (Scophthalmus maximus) leukocytes
6 páginas, 4 figuras Production of two eicosanoids derived from lipoxygenase and cyclooxygenase activities: leukotriene B4 (LTB4) and prostaglandin E2 (PGE2), respectively, have been simultaneously determined in turbot (Scophthalmus maximus) blood leucocyte and kidney macrophage supernatants by a re...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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1999
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Online Access: | http://hdl.handle.net/10261/57351 https://doi.org/10.1016/S0305-0491(99)00075-9 |
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ftcsic:oai:digital.csic.es:10261/57351 2024-02-11T10:08:25+01:00 Production of leukotriene B4 and prostaglandin E2 by turbot (Scophthalmus maximus) leukocytes Tafalla, Carolina Medina, Isabel Figueras Huerta, Antonio Novoa, Beatriz 1999 http://hdl.handle.net/10261/57351 https://doi.org/10.1016/S0305-0491(99)00075-9 en eng Elsevier http://dx.doi.org/10.1016/S0305-0491(99)00075-9 Comparative Biochemistry and Physiology - B - Biochemistry and Molecular Biology 123(4): 351-356 (1999) 1096-4959 http://hdl.handle.net/10261/57351 doi:10.1016/S0305-0491(99)00075-9 none Leukocyte Fish Eicosanoids HPLC Leukotriene B4 Prostaglandin E2 Macrophage Scophthalmus maximus artículo http://purl.org/coar/resource_type/c_6501 1999 ftcsic https://doi.org/10.1016/S0305-0491(99)00075-9 2024-01-16T09:41:12Z 6 páginas, 4 figuras Production of two eicosanoids derived from lipoxygenase and cyclooxygenase activities: leukotriene B4 (LTB4) and prostaglandin E2 (PGE2), respectively, have been simultaneously determined in turbot (Scophthalmus maximus) blood leucocyte and kidney macrophage supernatants by a reverse phase high performance liquid chromatography (HPLC) system coupled with a Diode–Array detector. Levels of LTB4 after calcium ionophore challenge were 4.08 ng ml 1 in blood leukocyte supernatants and 0.25 ng ml 1 in kidney macrophage supernatants. The levels found for PGE2 were 428.23 and 606.67 ng ml 1 in blood leukocytes and kidney macrophage supernatants, respectively. When blood leukocytes were treated with the respective inhibitors for the enzymes implicated on the synthesis of both compounds an inhibition of 90.35% was observed for PGE2 and 76.44% for LTB4. The detection limit of the method was 0.15 ng ml 1 for LTB4 and 50 ng ml 1 for PGE2. This research was supported by grant MAR 96-1775 from the Comisio´n Interministerial de Ciencia y Tecnologı´a (CICYT, Spain). Peer reviewed Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 123 4 351 356 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Leukocyte Fish Eicosanoids HPLC Leukotriene B4 Prostaglandin E2 Macrophage Scophthalmus maximus |
spellingShingle |
Leukocyte Fish Eicosanoids HPLC Leukotriene B4 Prostaglandin E2 Macrophage Scophthalmus maximus Tafalla, Carolina Medina, Isabel Figueras Huerta, Antonio Novoa, Beatriz Production of leukotriene B4 and prostaglandin E2 by turbot (Scophthalmus maximus) leukocytes |
topic_facet |
Leukocyte Fish Eicosanoids HPLC Leukotriene B4 Prostaglandin E2 Macrophage Scophthalmus maximus |
description |
6 páginas, 4 figuras Production of two eicosanoids derived from lipoxygenase and cyclooxygenase activities: leukotriene B4 (LTB4) and prostaglandin E2 (PGE2), respectively, have been simultaneously determined in turbot (Scophthalmus maximus) blood leucocyte and kidney macrophage supernatants by a reverse phase high performance liquid chromatography (HPLC) system coupled with a Diode–Array detector. Levels of LTB4 after calcium ionophore challenge were 4.08 ng ml 1 in blood leukocyte supernatants and 0.25 ng ml 1 in kidney macrophage supernatants. The levels found for PGE2 were 428.23 and 606.67 ng ml 1 in blood leukocytes and kidney macrophage supernatants, respectively. When blood leukocytes were treated with the respective inhibitors for the enzymes implicated on the synthesis of both compounds an inhibition of 90.35% was observed for PGE2 and 76.44% for LTB4. The detection limit of the method was 0.15 ng ml 1 for LTB4 and 50 ng ml 1 for PGE2. This research was supported by grant MAR 96-1775 from the Comisio´n Interministerial de Ciencia y Tecnologı´a (CICYT, Spain). Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Tafalla, Carolina Medina, Isabel Figueras Huerta, Antonio Novoa, Beatriz |
author_facet |
Tafalla, Carolina Medina, Isabel Figueras Huerta, Antonio Novoa, Beatriz |
author_sort |
Tafalla, Carolina |
title |
Production of leukotriene B4 and prostaglandin E2 by turbot (Scophthalmus maximus) leukocytes |
title_short |
Production of leukotriene B4 and prostaglandin E2 by turbot (Scophthalmus maximus) leukocytes |
title_full |
Production of leukotriene B4 and prostaglandin E2 by turbot (Scophthalmus maximus) leukocytes |
title_fullStr |
Production of leukotriene B4 and prostaglandin E2 by turbot (Scophthalmus maximus) leukocytes |
title_full_unstemmed |
Production of leukotriene B4 and prostaglandin E2 by turbot (Scophthalmus maximus) leukocytes |
title_sort |
production of leukotriene b4 and prostaglandin e2 by turbot (scophthalmus maximus) leukocytes |
publisher |
Elsevier |
publishDate |
1999 |
url |
http://hdl.handle.net/10261/57351 https://doi.org/10.1016/S0305-0491(99)00075-9 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://dx.doi.org/10.1016/S0305-0491(99)00075-9 Comparative Biochemistry and Physiology - B - Biochemistry and Molecular Biology 123(4): 351-356 (1999) 1096-4959 http://hdl.handle.net/10261/57351 doi:10.1016/S0305-0491(99)00075-9 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/S0305-0491(99)00075-9 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
123 |
container_issue |
4 |
container_start_page |
351 |
op_container_end_page |
356 |
_version_ |
1790607737548177408 |