Possible role of LTB4 in the antiviral activity of turbot (Scophthalmus maximus) leukocyte-derived supernatants against viral hemorrhagic septicemia virus (VHSV)
11 páginas, 4 figuras, 1 tabla Turbot (Scophthalmus maximus) blood leukocyte-derived supernatants were tested for antiviral activity against viral hemorrhagic septicemia virus (VHSV). The assays were performed by quantifying the effect of the supernatants on the replication of VHSV in the rainbow tr...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://hdl.handle.net/10261/56439 https://doi.org/10.1016/S0145-305X(01)00076-3 |
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ftcsic:oai:digital.csic.es:10261/56439 2024-02-11T10:08:26+01:00 Possible role of LTB4 in the antiviral activity of turbot (Scophthalmus maximus) leukocyte-derived supernatants against viral hemorrhagic septicemia virus (VHSV) Tafalla, Carolina Figueras Huerta, Antonio Novoa, Beatriz 2002 http://hdl.handle.net/10261/56439 https://doi.org/10.1016/S0145-305X(01)00076-3 en eng Elsevier http://dx.doi.org/10.1016/S0145-305X(01)00076-3 Developmental and Comparative Immunology 26(3): 283-293 (2002) 0145-305X http://hdl.handle.net/10261/56439 doi:10.1016/S0145-305X(01)00076-3 none artículo http://purl.org/coar/resource_type/c_6501 2002 ftcsic https://doi.org/10.1016/S0145-305X(01)00076-3 2024-01-16T09:40:48Z 11 páginas, 4 figuras, 1 tabla Turbot (Scophthalmus maximus) blood leukocyte-derived supernatants were tested for antiviral activity against viral hemorrhagic septicemia virus (VHSV). The assays were performed by quantifying the effect of the supernatants on the replication of VHSV in the rainbow trout (Oncorhynchus mykiss) cell line, RTG-2. Supernatants were obtained by incubating the leukocytes for 17 h at 18°C in L-15 medium supplemented with 5% fetal calf serum (FCS). Testing of leukocyte supernatants indicated that antiviral activity against VHSV resulted in a viral titer reduction of 72.1%. After the supernatants were extracted with calcium ionophore A23187 treatment, the antiviral activity significantly increased, resulting in a viral titer reduction of 99.9%. In order to determine the nature of this antiviral activity, supernatants were produced from leukocytes treated for 17 h with inhibitors of eicosanoid biosynthesis, reactive oxygen intermediates and nitric oxide (NO) production. None of the inhibitors significantly suppressed the supernatant antiviral activity. The presence of oxygen radicals and NO was measured in the case of co-cultures of leukocytes and RTG-2 cells, but no significant differences were found in the VHSV-infected co-cultures compared to non-infected controls. Since previous work demonstrated that leukotriene B4 (LTB4) was present in turbot blood leukocyte-derived supernatants, we assessed the effect of the VHSV in vitro infection on turbot leukocyte LTB4 production by high performance liquid chromatography (HPLC). The levels of LTB4 were significantly increased in the supernatants after VHSV infection. Furthermore, exogenous LTB4 significantly inhibited VHSV replication in RTG-2 cells. These findings suggest that LTB4 may play a significant role in VHSV replication This research was supported by grant MAR 96-1775 from the Comisión Interministerial de Ciencia y Tecnologı́a (CICYT, Spain). Peer reviewed Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Developmental & Comparative Immunology 26 3 283 293 |
institution |
Open Polar |
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Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
11 páginas, 4 figuras, 1 tabla Turbot (Scophthalmus maximus) blood leukocyte-derived supernatants were tested for antiviral activity against viral hemorrhagic septicemia virus (VHSV). The assays were performed by quantifying the effect of the supernatants on the replication of VHSV in the rainbow trout (Oncorhynchus mykiss) cell line, RTG-2. Supernatants were obtained by incubating the leukocytes for 17 h at 18°C in L-15 medium supplemented with 5% fetal calf serum (FCS). Testing of leukocyte supernatants indicated that antiviral activity against VHSV resulted in a viral titer reduction of 72.1%. After the supernatants were extracted with calcium ionophore A23187 treatment, the antiviral activity significantly increased, resulting in a viral titer reduction of 99.9%. In order to determine the nature of this antiviral activity, supernatants were produced from leukocytes treated for 17 h with inhibitors of eicosanoid biosynthesis, reactive oxygen intermediates and nitric oxide (NO) production. None of the inhibitors significantly suppressed the supernatant antiviral activity. The presence of oxygen radicals and NO was measured in the case of co-cultures of leukocytes and RTG-2 cells, but no significant differences were found in the VHSV-infected co-cultures compared to non-infected controls. Since previous work demonstrated that leukotriene B4 (LTB4) was present in turbot blood leukocyte-derived supernatants, we assessed the effect of the VHSV in vitro infection on turbot leukocyte LTB4 production by high performance liquid chromatography (HPLC). The levels of LTB4 were significantly increased in the supernatants after VHSV infection. Furthermore, exogenous LTB4 significantly inhibited VHSV replication in RTG-2 cells. These findings suggest that LTB4 may play a significant role in VHSV replication This research was supported by grant MAR 96-1775 from the Comisión Interministerial de Ciencia y Tecnologı́a (CICYT, Spain). Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Tafalla, Carolina Figueras Huerta, Antonio Novoa, Beatriz |
spellingShingle |
Tafalla, Carolina Figueras Huerta, Antonio Novoa, Beatriz Possible role of LTB4 in the antiviral activity of turbot (Scophthalmus maximus) leukocyte-derived supernatants against viral hemorrhagic septicemia virus (VHSV) |
author_facet |
Tafalla, Carolina Figueras Huerta, Antonio Novoa, Beatriz |
author_sort |
Tafalla, Carolina |
title |
Possible role of LTB4 in the antiviral activity of turbot (Scophthalmus maximus) leukocyte-derived supernatants against viral hemorrhagic septicemia virus (VHSV) |
title_short |
Possible role of LTB4 in the antiviral activity of turbot (Scophthalmus maximus) leukocyte-derived supernatants against viral hemorrhagic septicemia virus (VHSV) |
title_full |
Possible role of LTB4 in the antiviral activity of turbot (Scophthalmus maximus) leukocyte-derived supernatants against viral hemorrhagic septicemia virus (VHSV) |
title_fullStr |
Possible role of LTB4 in the antiviral activity of turbot (Scophthalmus maximus) leukocyte-derived supernatants against viral hemorrhagic septicemia virus (VHSV) |
title_full_unstemmed |
Possible role of LTB4 in the antiviral activity of turbot (Scophthalmus maximus) leukocyte-derived supernatants against viral hemorrhagic septicemia virus (VHSV) |
title_sort |
possible role of ltb4 in the antiviral activity of turbot (scophthalmus maximus) leukocyte-derived supernatants against viral hemorrhagic septicemia virus (vhsv) |
publisher |
Elsevier |
publishDate |
2002 |
url |
http://hdl.handle.net/10261/56439 https://doi.org/10.1016/S0145-305X(01)00076-3 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://dx.doi.org/10.1016/S0145-305X(01)00076-3 Developmental and Comparative Immunology 26(3): 283-293 (2002) 0145-305X http://hdl.handle.net/10261/56439 doi:10.1016/S0145-305X(01)00076-3 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/S0145-305X(01)00076-3 |
container_title |
Developmental & Comparative Immunology |
container_volume |
26 |
container_issue |
3 |
container_start_page |
283 |
op_container_end_page |
293 |
_version_ |
1790607765422473216 |