A model for experimental infections with Vibrio (Listonella) anguillarum in first feeding turbot (Scophthalmus maximus L.) larvae under hatchery conditions
12 páginas, 4 figuras, 4 tablas Vibriosis is one of the main causes of mortality in all stages of fish. In larvae, further improvements on the control and knowledge of bacterial diseases require the development of models for experimental infections. A reproducible model for in vivo infections of fir...
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Language: | English |
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Online Access: | http://hdl.handle.net/10261/51514 https://doi.org/10.1016/j.aquaculture.2005.04.050 |
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ftcsic:oai:digital.csic.es:10261/51514 2024-02-11T10:08:25+01:00 A model for experimental infections with Vibrio (Listonella) anguillarum in first feeding turbot (Scophthalmus maximus L.) larvae under hatchery conditions Planas, Miguel Pérez-Lorenzo, María Vázquez, José Antonio Pintado, José 2005 http://hdl.handle.net/10261/51514 https://doi.org/10.1016/j.aquaculture.2005.04.050 en eng Elsevier http://dx.doi.org/10.1016/j.aquaculture.2005.04.050 Aquaculture 250(1-2): 232-243 (2005) 0044-8486 http://hdl.handle.net/10261/51514 doi:10.1016/j.aquaculture.2005.04.050 open Infection model Larval rearings Vibrio anguillarum Rotifer Turbot Scophthalmus maximus artículo http://purl.org/coar/resource_type/c_6501 2005 ftcsic https://doi.org/10.1016/j.aquaculture.2005.04.050 2024-01-16T09:38:38Z 12 páginas, 4 figuras, 4 tablas Vibriosis is one of the main causes of mortality in all stages of fish. In larvae, further improvements on the control and knowledge of bacterial diseases require the development of models for experimental infections. A reproducible model for in vivo infections of first feeding turbot (Scophthalmus maximus) larvae with the pathogen Vibrio (Listonella) anguillarum is described. Experimental challenges were carried out under hatchery conditions considering different ways of delivery of V. (Listonella) anguillarum strain 90-11-287 serotype O1 to turbot larvae: via rotifers (orally) and via water of the larval rearing tanks (bath). The effect of the supernatant of the bacteria culture was also considered and discussed. The model proposed is based on the incorporation of the bacteria into previously enriched rotifers and the delivery to the larvae during the rotifer feeding period. The effect of infections was already evident 24–72 h after the first challenged day. Final mortalities (days 8–14) in infected larvae were 56–72% with respect to unchallenged larvae. Mortalities achieved by applying the protocol of infection are reliable, reproducible and adequate for experimental purposes. The model of infection via rotifers proposed in this study is a useful tool for experimental purposes in the larval rearing of marine fish. This work was supported by funds of the European Commission (Project PROBE, Contract No. Q5RS-2000-31457). Dr. J.A. Va´zquez had a postdoctoral contract (CSIC-I3P-PC 2003) financed by the European Social Fund. Peer reviewed Article in Journal/Newspaper Scophthalmus maximus Turbot Rotifer Digital.CSIC (Spanish National Research Council) Aquaculture 250 1-2 232 243 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Infection model Larval rearings Vibrio anguillarum Rotifer Turbot Scophthalmus maximus |
spellingShingle |
Infection model Larval rearings Vibrio anguillarum Rotifer Turbot Scophthalmus maximus Planas, Miguel Pérez-Lorenzo, María Vázquez, José Antonio Pintado, José A model for experimental infections with Vibrio (Listonella) anguillarum in first feeding turbot (Scophthalmus maximus L.) larvae under hatchery conditions |
topic_facet |
Infection model Larval rearings Vibrio anguillarum Rotifer Turbot Scophthalmus maximus |
description |
12 páginas, 4 figuras, 4 tablas Vibriosis is one of the main causes of mortality in all stages of fish. In larvae, further improvements on the control and knowledge of bacterial diseases require the development of models for experimental infections. A reproducible model for in vivo infections of first feeding turbot (Scophthalmus maximus) larvae with the pathogen Vibrio (Listonella) anguillarum is described. Experimental challenges were carried out under hatchery conditions considering different ways of delivery of V. (Listonella) anguillarum strain 90-11-287 serotype O1 to turbot larvae: via rotifers (orally) and via water of the larval rearing tanks (bath). The effect of the supernatant of the bacteria culture was also considered and discussed. The model proposed is based on the incorporation of the bacteria into previously enriched rotifers and the delivery to the larvae during the rotifer feeding period. The effect of infections was already evident 24–72 h after the first challenged day. Final mortalities (days 8–14) in infected larvae were 56–72% with respect to unchallenged larvae. Mortalities achieved by applying the protocol of infection are reliable, reproducible and adequate for experimental purposes. The model of infection via rotifers proposed in this study is a useful tool for experimental purposes in the larval rearing of marine fish. This work was supported by funds of the European Commission (Project PROBE, Contract No. Q5RS-2000-31457). Dr. J.A. Va´zquez had a postdoctoral contract (CSIC-I3P-PC 2003) financed by the European Social Fund. Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Planas, Miguel Pérez-Lorenzo, María Vázquez, José Antonio Pintado, José |
author_facet |
Planas, Miguel Pérez-Lorenzo, María Vázquez, José Antonio Pintado, José |
author_sort |
Planas, Miguel |
title |
A model for experimental infections with Vibrio (Listonella) anguillarum in first feeding turbot (Scophthalmus maximus L.) larvae under hatchery conditions |
title_short |
A model for experimental infections with Vibrio (Listonella) anguillarum in first feeding turbot (Scophthalmus maximus L.) larvae under hatchery conditions |
title_full |
A model for experimental infections with Vibrio (Listonella) anguillarum in first feeding turbot (Scophthalmus maximus L.) larvae under hatchery conditions |
title_fullStr |
A model for experimental infections with Vibrio (Listonella) anguillarum in first feeding turbot (Scophthalmus maximus L.) larvae under hatchery conditions |
title_full_unstemmed |
A model for experimental infections with Vibrio (Listonella) anguillarum in first feeding turbot (Scophthalmus maximus L.) larvae under hatchery conditions |
title_sort |
model for experimental infections with vibrio (listonella) anguillarum in first feeding turbot (scophthalmus maximus l.) larvae under hatchery conditions |
publisher |
Elsevier |
publishDate |
2005 |
url |
http://hdl.handle.net/10261/51514 https://doi.org/10.1016/j.aquaculture.2005.04.050 |
genre |
Scophthalmus maximus Turbot Rotifer |
genre_facet |
Scophthalmus maximus Turbot Rotifer |
op_relation |
http://dx.doi.org/10.1016/j.aquaculture.2005.04.050 Aquaculture 250(1-2): 232-243 (2005) 0044-8486 http://hdl.handle.net/10261/51514 doi:10.1016/j.aquaculture.2005.04.050 |
op_rights |
open |
op_doi |
https://doi.org/10.1016/j.aquaculture.2005.04.050 |
container_title |
Aquaculture |
container_volume |
250 |
container_issue |
1-2 |
container_start_page |
232 |
op_container_end_page |
243 |
_version_ |
1790607746600534016 |