Study of Genetic Diversity of Eukaryotic Picoplankton in Different Oceanic Regions by Small-Subunit rRNA Gene Cloning and Sequencing
10 pages, 3 figures, 4 tables Very small eukaryotic organisms (picoeukaryotes) are fundamental components of marine planktonic systems, often accounting for a significant fraction of the biomass and activity in a system. Their identity, however, has remained elusive, since the small cells lack morph...
Published in: | Applied and Environmental Microbiology |
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American Society for Microbiology
2001
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Online Access: | http://hdl.handle.net/10261/49543 https://doi.org/10.1128/AEM.67.7.2932-2941.2001 |
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ftcsic:oai:digital.csic.es:10261/49543 2024-02-11T10:06:18+01:00 Study of Genetic Diversity of Eukaryotic Picoplankton in Different Oceanic Regions by Small-Subunit rRNA Gene Cloning and Sequencing Díez, Beatriz Pedrós-Alió, Carlos Massana, Ramon 2001-07 http://hdl.handle.net/10261/49543 https://doi.org/10.1128/AEM.67.7.2932-2941.2001 en eng American Society for Microbiology https://doi.org/10.1128/AEM.67.7.2932-2941.2001 Applied and Environmental Microbiology 67(7): 2932-2941 (2001) 0099-2240 http://hdl.handle.net/10261/49543 doi:10.1128/AEM.67.7.2932-2941.2001 1098-5336 11425705 none artículo http://purl.org/coar/resource_type/c_6501 2001 ftcsic https://doi.org/10.1128/AEM.67.7.2932-2941.2001 2024-01-16T09:37:45Z 10 pages, 3 figures, 4 tables Very small eukaryotic organisms (picoeukaryotes) are fundamental components of marine planktonic systems, often accounting for a significant fraction of the biomass and activity in a system. Their identity, however, has remained elusive, since the small cells lack morphological features for identification. We determined the diversity of marine picoeukaryotes by sequencing cloned 18S rRNA genes in five genetic libraries from North Atlantic, Southern Ocean, and Mediterranean Sea surface waters. Picoplankton were obtained by filter size fractionation, a step that excluded most large eukaryotes and recovered most picoeukaryotes. Genetic libraries of eukaryotic ribosomal DNA were screened by restriction fragment length polymorphism analysis, and at least one clone of each operational taxonomic unit (OTU) was partially sequenced. In general, the phylogenetic diversity in each library was rather great, and each library included many different OTUs and members of very distantly related phylogenetic groups. Of 225 eukaryotic clones, 126 were affiliated with algal classes, especially the Prasinophyceae, the Prymnesiophyceae, the Bacillariophyceae, and the Dinophyceae. A minor fraction (27 clones) was affiliated with clearly heterotrophic organisms, such as ciliates, the chrysomonad Paraphysomonas, cercomonads, and fungi. There were two relatively abundant novel lineages, novel stramenopiles (53 clones) and novel alveolates (19 clones). These lineages are very different from any organism that has been isolated, suggesting that there are previously unknown picoeukaryotes. Prasinophytes and novel stramenopile clones were very abundant in all of the libraries analyzed. These findings underscore the importance of attempts to grow the small eukaryotic plankton in pure culture This work was funded by EU contracts MIDAS (MAS3-CT97-00154) and PICODIV (EVK3-CT1999-00021). The North Atlantic samples were collected during the ACSOE NAE cruise of the RRS Discovery funded by the British NERC and ... Article in Journal/Newspaper North Atlantic Southern Ocean Digital.CSIC (Spanish National Research Council) Southern Ocean Applied and Environmental Microbiology 67 7 2932 2941 |
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Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
10 pages, 3 figures, 4 tables Very small eukaryotic organisms (picoeukaryotes) are fundamental components of marine planktonic systems, often accounting for a significant fraction of the biomass and activity in a system. Their identity, however, has remained elusive, since the small cells lack morphological features for identification. We determined the diversity of marine picoeukaryotes by sequencing cloned 18S rRNA genes in five genetic libraries from North Atlantic, Southern Ocean, and Mediterranean Sea surface waters. Picoplankton were obtained by filter size fractionation, a step that excluded most large eukaryotes and recovered most picoeukaryotes. Genetic libraries of eukaryotic ribosomal DNA were screened by restriction fragment length polymorphism analysis, and at least one clone of each operational taxonomic unit (OTU) was partially sequenced. In general, the phylogenetic diversity in each library was rather great, and each library included many different OTUs and members of very distantly related phylogenetic groups. Of 225 eukaryotic clones, 126 were affiliated with algal classes, especially the Prasinophyceae, the Prymnesiophyceae, the Bacillariophyceae, and the Dinophyceae. A minor fraction (27 clones) was affiliated with clearly heterotrophic organisms, such as ciliates, the chrysomonad Paraphysomonas, cercomonads, and fungi. There were two relatively abundant novel lineages, novel stramenopiles (53 clones) and novel alveolates (19 clones). These lineages are very different from any organism that has been isolated, suggesting that there are previously unknown picoeukaryotes. Prasinophytes and novel stramenopile clones were very abundant in all of the libraries analyzed. These findings underscore the importance of attempts to grow the small eukaryotic plankton in pure culture This work was funded by EU contracts MIDAS (MAS3-CT97-00154) and PICODIV (EVK3-CT1999-00021). The North Atlantic samples were collected during the ACSOE NAE cruise of the RRS Discovery funded by the British NERC and ... |
format |
Article in Journal/Newspaper |
author |
Díez, Beatriz Pedrós-Alió, Carlos Massana, Ramon |
spellingShingle |
Díez, Beatriz Pedrós-Alió, Carlos Massana, Ramon Study of Genetic Diversity of Eukaryotic Picoplankton in Different Oceanic Regions by Small-Subunit rRNA Gene Cloning and Sequencing |
author_facet |
Díez, Beatriz Pedrós-Alió, Carlos Massana, Ramon |
author_sort |
Díez, Beatriz |
title |
Study of Genetic Diversity of Eukaryotic Picoplankton in Different Oceanic Regions by Small-Subunit rRNA Gene Cloning and Sequencing |
title_short |
Study of Genetic Diversity of Eukaryotic Picoplankton in Different Oceanic Regions by Small-Subunit rRNA Gene Cloning and Sequencing |
title_full |
Study of Genetic Diversity of Eukaryotic Picoplankton in Different Oceanic Regions by Small-Subunit rRNA Gene Cloning and Sequencing |
title_fullStr |
Study of Genetic Diversity of Eukaryotic Picoplankton in Different Oceanic Regions by Small-Subunit rRNA Gene Cloning and Sequencing |
title_full_unstemmed |
Study of Genetic Diversity of Eukaryotic Picoplankton in Different Oceanic Regions by Small-Subunit rRNA Gene Cloning and Sequencing |
title_sort |
study of genetic diversity of eukaryotic picoplankton in different oceanic regions by small-subunit rrna gene cloning and sequencing |
publisher |
American Society for Microbiology |
publishDate |
2001 |
url |
http://hdl.handle.net/10261/49543 https://doi.org/10.1128/AEM.67.7.2932-2941.2001 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
North Atlantic Southern Ocean |
genre_facet |
North Atlantic Southern Ocean |
op_relation |
https://doi.org/10.1128/AEM.67.7.2932-2941.2001 Applied and Environmental Microbiology 67(7): 2932-2941 (2001) 0099-2240 http://hdl.handle.net/10261/49543 doi:10.1128/AEM.67.7.2932-2941.2001 1098-5336 11425705 |
op_rights |
none |
op_doi |
https://doi.org/10.1128/AEM.67.7.2932-2941.2001 |
container_title |
Applied and Environmental Microbiology |
container_volume |
67 |
container_issue |
7 |
container_start_page |
2932 |
op_container_end_page |
2941 |
_version_ |
1790603942438109184 |