Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development
12 páginas, 4 figuras, 3 tablas. Epidermal, branchial and digestive mucous cells, and the gastric glands of larvae/postlarvae (from hatching until 45 days posthatching) of three fish species (two teleostean and a chondrostean) were investigated using conventional histochemical methods (periodic acid...
Published in: | European Journal of Histochemistry |
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Language: | English |
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PAGEPress
2001
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Online Access: | http://hdl.handle.net/10261/48340 https://doi.org/10.4081/1637 |
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ftcsic:oai:digital.csic.es:10261/48340 2024-02-11T10:08:34+01:00 Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development Sarasquete, Carmen Gisbert, Enric Ribeiro, Laura Vieira, L. Dinis, M. T. 2001 http://hdl.handle.net/10261/48340 https://doi.org/10.4081/1637 en eng PAGEPress http://dx.doi.org/10.4081/1637 European Journal of Histochemistry 45(3): 267-278 (2001) 1121-760X PMID: 11759813 http://hdl.handle.net/10261/48340 doi:10.4081/1637 2038-8306 open Histochemistry Carbohydrates Proteins Glycoproteins Mucous cells Gastric glands Sea bream Sole Sturgeon Larvae artículo http://purl.org/coar/resource_type/c_6501 2001 ftcsic https://doi.org/10.4081/1637 2024-01-16T09:37:15Z 12 páginas, 4 figuras, 3 tablas. Epidermal, branchial and digestive mucous cells, and the gastric glands of larvae/postlarvae (from hatching until 45 days posthatching) of three fish species (two teleostean and a chondrostean) were investigated using conventional histochemical methods (periodic acid schiff -PAS-, diastase-PAS; alcian blue pH 0.5, 1 and 2.5) in order to distinguish neutral and acidic (carboxylated and sulphated) glycoconjugates, as well as bromophenol blue reaction for identification of proteins. Additionally, the presence and distribution of sugar residues in the oligosaccharide side chains of glycoconjugates were investigated using horseradish peroxidase (HPR)-conjugated lectins (Con A, DBA, WGA and UEA-I). Most mucous cells (digestive, epidermal and branchial) of Siberian sturgeon, Acipenser baeri, sea bream, Sparus aurata and Senegal sole, Solea senegalensis larvae were PAS- and alcian blue- (pH 2.5 and 0.5) positive, with small variations between organs/tissues and species. Bromophenol blue reaction (general proteins) was positive in a minority of the mucous cells, usually in those cells which were PAS-negative. Proteins rich in sulphydryl (-SH) and/or disulphide (-S-S-) groups related with the glycoprotein nature of the glycoconjugates present in mucous cells were also observed. Epidermal, branchial and digestive mucous cells of all studied larvae did not contain glycogen or lipids. Con A lectin staining was negative in all mucous cells types of sea bream and sole, but oesophageal mucous cell of sturgeon were reactive to different lectin reactions, suggesting the presence of mannose -Man- and/or glucose -Glc-, L-fucose -Fuc- N-acetyl-D-galactosamine -GalNAc-, as well as N-acetyl-D-glucosamine- GlcNAc - and/or sialic acid -NANA- residues. Digestive mucous cells of all studied larvae were positive to WGA and DBA lectins. Epidermal and branchial mucous cells of sea bream and sole were Con A, DBA and UEA-I unreactive. However, mucous cells of sturgeon larvae were stained with UEA-I lectin. ... Article in Journal/Newspaper Siberian sturgeon Digital.CSIC (Spanish National Research Council) European Journal of Histochemistry 45 3 267 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Histochemistry Carbohydrates Proteins Glycoproteins Mucous cells Gastric glands Sea bream Sole Sturgeon Larvae |
spellingShingle |
Histochemistry Carbohydrates Proteins Glycoproteins Mucous cells Gastric glands Sea bream Sole Sturgeon Larvae Sarasquete, Carmen Gisbert, Enric Ribeiro, Laura Vieira, L. Dinis, M. T. Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development |
topic_facet |
Histochemistry Carbohydrates Proteins Glycoproteins Mucous cells Gastric glands Sea bream Sole Sturgeon Larvae |
description |
12 páginas, 4 figuras, 3 tablas. Epidermal, branchial and digestive mucous cells, and the gastric glands of larvae/postlarvae (from hatching until 45 days posthatching) of three fish species (two teleostean and a chondrostean) were investigated using conventional histochemical methods (periodic acid schiff -PAS-, diastase-PAS; alcian blue pH 0.5, 1 and 2.5) in order to distinguish neutral and acidic (carboxylated and sulphated) glycoconjugates, as well as bromophenol blue reaction for identification of proteins. Additionally, the presence and distribution of sugar residues in the oligosaccharide side chains of glycoconjugates were investigated using horseradish peroxidase (HPR)-conjugated lectins (Con A, DBA, WGA and UEA-I). Most mucous cells (digestive, epidermal and branchial) of Siberian sturgeon, Acipenser baeri, sea bream, Sparus aurata and Senegal sole, Solea senegalensis larvae were PAS- and alcian blue- (pH 2.5 and 0.5) positive, with small variations between organs/tissues and species. Bromophenol blue reaction (general proteins) was positive in a minority of the mucous cells, usually in those cells which were PAS-negative. Proteins rich in sulphydryl (-SH) and/or disulphide (-S-S-) groups related with the glycoprotein nature of the glycoconjugates present in mucous cells were also observed. Epidermal, branchial and digestive mucous cells of all studied larvae did not contain glycogen or lipids. Con A lectin staining was negative in all mucous cells types of sea bream and sole, but oesophageal mucous cell of sturgeon were reactive to different lectin reactions, suggesting the presence of mannose -Man- and/or glucose -Glc-, L-fucose -Fuc- N-acetyl-D-galactosamine -GalNAc-, as well as N-acetyl-D-glucosamine- GlcNAc - and/or sialic acid -NANA- residues. Digestive mucous cells of all studied larvae were positive to WGA and DBA lectins. Epidermal and branchial mucous cells of sea bream and sole were Con A, DBA and UEA-I unreactive. However, mucous cells of sturgeon larvae were stained with UEA-I lectin. ... |
format |
Article in Journal/Newspaper |
author |
Sarasquete, Carmen Gisbert, Enric Ribeiro, Laura Vieira, L. Dinis, M. T. |
author_facet |
Sarasquete, Carmen Gisbert, Enric Ribeiro, Laura Vieira, L. Dinis, M. T. |
author_sort |
Sarasquete, Carmen |
title |
Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development |
title_short |
Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development |
title_full |
Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development |
title_fullStr |
Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development |
title_full_unstemmed |
Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development |
title_sort |
glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, sparus aurata, senegal sole, solea senegalensis and siberian sturgeon, acipenser baeri development |
publisher |
PAGEPress |
publishDate |
2001 |
url |
http://hdl.handle.net/10261/48340 https://doi.org/10.4081/1637 |
genre |
Siberian sturgeon |
genre_facet |
Siberian sturgeon |
op_relation |
http://dx.doi.org/10.4081/1637 European Journal of Histochemistry 45(3): 267-278 (2001) 1121-760X PMID: 11759813 http://hdl.handle.net/10261/48340 doi:10.4081/1637 2038-8306 |
op_rights |
open |
op_doi |
https://doi.org/10.4081/1637 |
container_title |
European Journal of Histochemistry |
container_volume |
45 |
container_issue |
3 |
container_start_page |
267 |
_version_ |
1790607980138332160 |