A novel hepcidin-like in turbot (Scophthalmus maximus L.) highly expressed after pathogen challenge but not after iron overload
11 páginas, 9 figuras, 1 tabla Hepcidins are antimicrobial peptides with an important role in the host innate immunity. Moreover, it has been reported that mammalian hepcidins present a dual-function being a key regulator in the iron homeostasis. Here, we describe the coding sequence of a novel hepc...
Published in: | Fish & Shellfish Immunology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2012
|
Subjects: | |
Online Access: | http://hdl.handle.net/10261/48027 https://doi.org/10.1016/j.fsi.2012.02.016 |
id |
ftcsic:oai:digital.csic.es:10261/48027 |
---|---|
record_format |
openpolar |
spelling |
ftcsic:oai:digital.csic.es:10261/48027 2024-02-11T10:08:26+01:00 A novel hepcidin-like in turbot (Scophthalmus maximus L.) highly expressed after pathogen challenge but not after iron overload Pereiro, Patricia Figueras Huerta, Antonio Novoa, Beatriz 2012 http://hdl.handle.net/10261/48027 https://doi.org/10.1016/j.fsi.2012.02.016 en eng Elsevier http://dx.doi.org/10.1016/j.fsi.2012.02.016 Fish and Shellfish Immunology 32(5): 879-889 (2012) 1050-4648 http://hdl.handle.net/10261/48027 doi:10.1016/j.fsi.2012.02.016 1095-9947 open Turbot Hepcidin Aeromonas salmonicida VHSV Iron artículo http://purl.org/coar/resource_type/c_6501 2012 ftcsic https://doi.org/10.1016/j.fsi.2012.02.016 2024-01-16T09:37:07Z 11 páginas, 9 figuras, 1 tabla Hepcidins are antimicrobial peptides with an important role in the host innate immunity. Moreover, it has been reported that mammalian hepcidins present a dual-function being a key regulator in the iron homeostasis. Here, we describe the coding sequence of a novel hepcidin-like peptide in turbot, Scophthalmus maximus. This molecule presents several differences with regard to the previously characterized hepcidin in this flatfish species and it has not the hypothetical iron regulatory sequence Q-S/I-H-L/I-S/A-L in the N-terminal region. Therefore we propose the existence of at least two types of hepcidin in turbot. Moreover, results revealed a higher variability in the mRNA sequences of the novel hepcidin compared with the other form. Constitutive expression of turbot hepcidins (Hepcidin-1 and Hepcidin-2) was analyzed in several tissues and as expected, both molecules were highly represented in liver. On the other hand, the effect of three different stimuli (bacterial or viral infection and iron overloading) in the level of hepcidin mRNA was also examined and a differential response to pathogens and iron was observed. Whereas both hepcidins were affected by pathogen challenge, only Hepcidin-1 was up-regulated after iron overloading. Therefore, this and other evidences suggest that these peptides could be involved in different functions covering the dual role of mammalian hepcidins. Funding from the project CSD2007-00002 “Aquagenomics” of the program Consolider-Ingenio 2010 from the Spanish Ministerio de Ciencia e Innovación. Peer reviewed Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Fish & Shellfish Immunology 32 5 879 889 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Turbot Hepcidin Aeromonas salmonicida VHSV Iron |
spellingShingle |
Turbot Hepcidin Aeromonas salmonicida VHSV Iron Pereiro, Patricia Figueras Huerta, Antonio Novoa, Beatriz A novel hepcidin-like in turbot (Scophthalmus maximus L.) highly expressed after pathogen challenge but not after iron overload |
topic_facet |
Turbot Hepcidin Aeromonas salmonicida VHSV Iron |
description |
11 páginas, 9 figuras, 1 tabla Hepcidins are antimicrobial peptides with an important role in the host innate immunity. Moreover, it has been reported that mammalian hepcidins present a dual-function being a key regulator in the iron homeostasis. Here, we describe the coding sequence of a novel hepcidin-like peptide in turbot, Scophthalmus maximus. This molecule presents several differences with regard to the previously characterized hepcidin in this flatfish species and it has not the hypothetical iron regulatory sequence Q-S/I-H-L/I-S/A-L in the N-terminal region. Therefore we propose the existence of at least two types of hepcidin in turbot. Moreover, results revealed a higher variability in the mRNA sequences of the novel hepcidin compared with the other form. Constitutive expression of turbot hepcidins (Hepcidin-1 and Hepcidin-2) was analyzed in several tissues and as expected, both molecules were highly represented in liver. On the other hand, the effect of three different stimuli (bacterial or viral infection and iron overloading) in the level of hepcidin mRNA was also examined and a differential response to pathogens and iron was observed. Whereas both hepcidins were affected by pathogen challenge, only Hepcidin-1 was up-regulated after iron overloading. Therefore, this and other evidences suggest that these peptides could be involved in different functions covering the dual role of mammalian hepcidins. Funding from the project CSD2007-00002 “Aquagenomics” of the program Consolider-Ingenio 2010 from the Spanish Ministerio de Ciencia e Innovación. Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Pereiro, Patricia Figueras Huerta, Antonio Novoa, Beatriz |
author_facet |
Pereiro, Patricia Figueras Huerta, Antonio Novoa, Beatriz |
author_sort |
Pereiro, Patricia |
title |
A novel hepcidin-like in turbot (Scophthalmus maximus L.) highly expressed after pathogen challenge but not after iron overload |
title_short |
A novel hepcidin-like in turbot (Scophthalmus maximus L.) highly expressed after pathogen challenge but not after iron overload |
title_full |
A novel hepcidin-like in turbot (Scophthalmus maximus L.) highly expressed after pathogen challenge but not after iron overload |
title_fullStr |
A novel hepcidin-like in turbot (Scophthalmus maximus L.) highly expressed after pathogen challenge but not after iron overload |
title_full_unstemmed |
A novel hepcidin-like in turbot (Scophthalmus maximus L.) highly expressed after pathogen challenge but not after iron overload |
title_sort |
novel hepcidin-like in turbot (scophthalmus maximus l.) highly expressed after pathogen challenge but not after iron overload |
publisher |
Elsevier |
publishDate |
2012 |
url |
http://hdl.handle.net/10261/48027 https://doi.org/10.1016/j.fsi.2012.02.016 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://dx.doi.org/10.1016/j.fsi.2012.02.016 Fish and Shellfish Immunology 32(5): 879-889 (2012) 1050-4648 http://hdl.handle.net/10261/48027 doi:10.1016/j.fsi.2012.02.016 1095-9947 |
op_rights |
open |
op_doi |
https://doi.org/10.1016/j.fsi.2012.02.016 |
container_title |
Fish & Shellfish Immunology |
container_volume |
32 |
container_issue |
5 |
container_start_page |
879 |
op_container_end_page |
889 |
_version_ |
1790607761393844224 |