A whole-cell hypersensitive biosensor for beta-lactams based on the AmpR-AmpC regulatory circuit from the Antarctic Pseudomonas sp. IB20
Detecting antibiotic residues is vital to minimize their impact. Yet, existing methods are complex and costly. Biosensors offer an alternative. While many biosensors detect various antibiotics, specific ones for beta-lactams are lacking. To address this gap, a biosensor based on the AmpC beta-lactam...
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2024
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Online Access: | http://hdl.handle.net/10261/359905 https://doi.org/10.1111/1751-7915.14385 https://api.elsevier.com/content/abstract/scopus_id/85181952138 |
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ftcsic:oai:digital.csic.es:10261/359905 2024-06-23T07:47:33+00:00 A whole-cell hypersensitive biosensor for beta-lactams based on the AmpR-AmpC regulatory circuit from the Antarctic Pseudomonas sp. IB20 Higuera-Llantén, Sebastián Alcalde-Rico, Manuel Vásquez-Ponce, Felipe Ibacache-Quiroga, Claudia Blazquez, Jesús Olivares-Pacheco, Jorge Universidad de Valparaíso Agencia Nacional de Investigación y Desarrollo (Chile) 2024-01 application/pdf http://hdl.handle.net/10261/359905 https://doi.org/10.1111/1751-7915.14385 https://api.elsevier.com/content/abstract/scopus_id/85181952138 en eng John Wiley & Sons Publisher's version The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1111/1751-7915.14385 https://doi.org/10.1111/1751-7915.14385 Sí Microbial Biotechnology 17(1): e14385 (2024) http://hdl.handle.net/10261/359905 doi:10.1111/1751-7915.14385 1751-7915 38197486 2-s2.0-85181952138 https://api.elsevier.com/content/abstract/scopus_id/85181952138 open artículo 2024 ftcsic https://doi.org/10.1111/1751-7915.14385 2024-06-11T23:56:09Z Detecting antibiotic residues is vital to minimize their impact. Yet, existing methods are complex and costly. Biosensors offer an alternative. While many biosensors detect various antibiotics, specific ones for beta-lactams are lacking. To address this gap, a biosensor based on the AmpC beta-lactamase regulation system (ampR-ampC) from Pseudomonas sp. IB20, an Antarctic isolate, was developed in this study. The AmpR-AmpC system is well-conserved in the genus Pseudomonas and has been extensively studied for its involvement in peptidoglycan recycling and beta-lactam resistance. To create the biosensor, the ampC coding sequence was replaced with the mCherry fluorescent protein as a reporter, resulting in a transcriptional fusion. This construct was then inserted into Escherichia coli SN0301, a beta-lactam hypersensitive strain, generating a whole-cell biosensor. The biosensor demonstrated dose-dependent detection of penicillins, cephalosporins and carbapenems. However, the most interesting aspect of this work is the high sensitivity presented by the biosensor in the detection of carbapenems, as it was able to detect 8 pg/mL of meropenem and 40 pg/mL of imipenem and reach levels of 1-10 ng/mL for penicillins and cephalosporins. This makes the biosensor a powerful tool for the detection of beta-lactam antibiotics, specifically carbapenems, in different matrices. This work was funded by the ANID Millennium Science Initiative/Millennium Initiative for Collaborative Research on Bacterial Resistance, MICROB-R, NCN17-08 and grant REDES190148 from ANID and DIUV-CIDI 4/2016 from Universidad de Valparaíso. Peer reviewed Article in Journal/Newspaper Antarc* Antarctic Digital.CSIC (Spanish National Research Council) Antarctic The Antarctic Valparaíso ENVELOPE(-62.983,-62.983,-64.833,-64.833) Microbial Biotechnology 17 1 |
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Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
Detecting antibiotic residues is vital to minimize their impact. Yet, existing methods are complex and costly. Biosensors offer an alternative. While many biosensors detect various antibiotics, specific ones for beta-lactams are lacking. To address this gap, a biosensor based on the AmpC beta-lactamase regulation system (ampR-ampC) from Pseudomonas sp. IB20, an Antarctic isolate, was developed in this study. The AmpR-AmpC system is well-conserved in the genus Pseudomonas and has been extensively studied for its involvement in peptidoglycan recycling and beta-lactam resistance. To create the biosensor, the ampC coding sequence was replaced with the mCherry fluorescent protein as a reporter, resulting in a transcriptional fusion. This construct was then inserted into Escherichia coli SN0301, a beta-lactam hypersensitive strain, generating a whole-cell biosensor. The biosensor demonstrated dose-dependent detection of penicillins, cephalosporins and carbapenems. However, the most interesting aspect of this work is the high sensitivity presented by the biosensor in the detection of carbapenems, as it was able to detect 8 pg/mL of meropenem and 40 pg/mL of imipenem and reach levels of 1-10 ng/mL for penicillins and cephalosporins. This makes the biosensor a powerful tool for the detection of beta-lactam antibiotics, specifically carbapenems, in different matrices. This work was funded by the ANID Millennium Science Initiative/Millennium Initiative for Collaborative Research on Bacterial Resistance, MICROB-R, NCN17-08 and grant REDES190148 from ANID and DIUV-CIDI 4/2016 from Universidad de Valparaíso. Peer reviewed |
author2 |
Universidad de Valparaíso Agencia Nacional de Investigación y Desarrollo (Chile) |
format |
Article in Journal/Newspaper |
author |
Higuera-Llantén, Sebastián Alcalde-Rico, Manuel Vásquez-Ponce, Felipe Ibacache-Quiroga, Claudia Blazquez, Jesús Olivares-Pacheco, Jorge |
spellingShingle |
Higuera-Llantén, Sebastián Alcalde-Rico, Manuel Vásquez-Ponce, Felipe Ibacache-Quiroga, Claudia Blazquez, Jesús Olivares-Pacheco, Jorge A whole-cell hypersensitive biosensor for beta-lactams based on the AmpR-AmpC regulatory circuit from the Antarctic Pseudomonas sp. IB20 |
author_facet |
Higuera-Llantén, Sebastián Alcalde-Rico, Manuel Vásquez-Ponce, Felipe Ibacache-Quiroga, Claudia Blazquez, Jesús Olivares-Pacheco, Jorge |
author_sort |
Higuera-Llantén, Sebastián |
title |
A whole-cell hypersensitive biosensor for beta-lactams based on the AmpR-AmpC regulatory circuit from the Antarctic Pseudomonas sp. IB20 |
title_short |
A whole-cell hypersensitive biosensor for beta-lactams based on the AmpR-AmpC regulatory circuit from the Antarctic Pseudomonas sp. IB20 |
title_full |
A whole-cell hypersensitive biosensor for beta-lactams based on the AmpR-AmpC regulatory circuit from the Antarctic Pseudomonas sp. IB20 |
title_fullStr |
A whole-cell hypersensitive biosensor for beta-lactams based on the AmpR-AmpC regulatory circuit from the Antarctic Pseudomonas sp. IB20 |
title_full_unstemmed |
A whole-cell hypersensitive biosensor for beta-lactams based on the AmpR-AmpC regulatory circuit from the Antarctic Pseudomonas sp. IB20 |
title_sort |
whole-cell hypersensitive biosensor for beta-lactams based on the ampr-ampc regulatory circuit from the antarctic pseudomonas sp. ib20 |
publisher |
John Wiley & Sons |
publishDate |
2024 |
url |
http://hdl.handle.net/10261/359905 https://doi.org/10.1111/1751-7915.14385 https://api.elsevier.com/content/abstract/scopus_id/85181952138 |
long_lat |
ENVELOPE(-62.983,-62.983,-64.833,-64.833) |
geographic |
Antarctic The Antarctic Valparaíso |
geographic_facet |
Antarctic The Antarctic Valparaíso |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Publisher's version The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1111/1751-7915.14385 https://doi.org/10.1111/1751-7915.14385 Sí Microbial Biotechnology 17(1): e14385 (2024) http://hdl.handle.net/10261/359905 doi:10.1111/1751-7915.14385 1751-7915 38197486 2-s2.0-85181952138 https://api.elsevier.com/content/abstract/scopus_id/85181952138 |
op_rights |
open |
op_doi |
https://doi.org/10.1111/1751-7915.14385 |
container_title |
Microbial Biotechnology |
container_volume |
17 |
container_issue |
1 |
_version_ |
1802651678077353984 |