Clues from the intestinal mucus proteome of Atlantic salmon to counter inflammation

• Published in: Journal of Proteomics
• Main Authors: Kiron, Viswanath, Kathiresan, Purushothaman, Fernandes, Jorge M. O., Sørensen, Mette, Vasanth, Ghana K., Lin, Qingsong, Lin, Qifeng, Lim, Teck Kwang, Dahle, Dalia, Dias, Jorge, Verlhac Trichet, Viviane
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 2022
• Subjects: Atlantic salmon; Intestinal inflammation; Soy saponin; Mucus proteome; Ribosomal proteins; Aminoacyl-tRNA ligases; Glycogen phosphorylase; Glutamine synthetase; Conserve and sustainably use the oceans; seas and marine resources for sustainable development
• Online Access: http://hdl.handle.net/10261/357924; https://doi.org/10.1016/j.jprot.2022.104487

11 pages, 5 figures, 1 table, supplementary data https://doi.org/10.1016/j.jprot.2022.104487.-- Data availability: The sequences are submitted in a public repository (PRIDE) Intestinal inflammation in Atlantic salmon was studied by profiling the intestine mucus proteome, employing iTRAQ and 2D LC-MS/MS approach. Two fish groups were fed soy saponin-containing (inflammation inducer) diets (SO and SP) and two control fish groups were fed diets devoid of soy saponin (CO and CP) for 36 days. The CP and SP diets contained a health additive. Inflammation characteristics in the intestine were milder in the SP-fed fish compared to the SO-fed fish. The SO group was characterised by alterations of many proteins. KEGG pathways such as phagosome and lipid binding were possibly affected in the SO group due to the higher abundant proteins like Integrin beta 2 precursor, Coronin 1A, Cathepsin S precursor, Vesicle-trafficking protein, and Neutrophil cytosol factors. On the other hand, the SP group had fewer altered proteins and inflammation characteristics; aminoacyl-tRNA biosynthesis and ribosome in the fish group were plausibly changed due to the higher abundance of many large and small subunit of ribosomes. Elevation of the abundance of ribosomal proteins, aminoacyl-tRNA ligases, and appropriate abundance of Glycogen phosphorylase and Glutamine synthetase could possibly alleviate intestinal inflammation. This study was undertaken as part of a collaborative research project “Molecular studies on the intestine of Atlantic salmon” between Nord University and DSM Nutritional Products, Switzerland, funded by the latter Peer reviewed