Single-cell genomics reveals the genome content of the uncultured cyanobacterial diazotroph UCYN-A3
ASLO Aquatic Sciences Meeting, Resilience and Recovery in Aquatic Systems, 4-9 June 2023, Palma de Mallorca, Spain The unicellular cyanobacteria Candidatus Atelocyanobacterium thalassa (hereafter, UCYN-A) are important nitrogen fixers that live in symbiosis with the haptophyte algae Braarudosphaera...
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Association for the Sciences of Limnology and Oceanography
2023
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ftcsic:oai:digital.csic.es:10261/354350 2024-05-19T07:48:33+00:00 Single-cell genomics reveals the genome content of the uncultured cyanobacterial diazotroph UCYN-A3 Vieyra Mexicano, Cinthya Bianchini, Giorgio Garczarek, Laurence Wincker, Patrick Acinas, Silvia G. Sánchez-Baracaldo, Patricia Zehr, Jonathan P. Cornejo-Castillo, Francisco M. 2023-06-07 http://hdl.handle.net/10261/354350 en eng Association for the Sciences of Limnology and Oceanography Sí ASLO Aquatic Sciences Meeting (2023) http://hdl.handle.net/10261/354350 none Conserve and sustainably use the oceans seas and marine resources for sustainable development comunicación de congreso 2023 ftcsic 2024-04-23T23:34:38Z ASLO Aquatic Sciences Meeting, Resilience and Recovery in Aquatic Systems, 4-9 June 2023, Palma de Mallorca, Spain The unicellular cyanobacteria Candidatus Atelocyanobacterium thalassa (hereafter, UCYN-A) are important nitrogen fixers that live in symbiosis with the haptophyte algae Braarudosphaera bigelowii and its close relative species. Although the UCYN-A phylogenetic group includes at least six sublineages, only the genomes of UCYN-A1 and UCYN-A2 had been sequenced to date. Therefore, despite its biogeochemical relevance, comparative genomics on the UCYN-A phylogenetic group has been limited by the poor representation of genomic data. Here, using a high-throughput DNA sequencing approach on a single sorted cell from the South Atlantic Ocean, we reconstructed about 65% of the genome of the UCYN-A3 sublineage. We show that although the genomic content of UCYN-A3 is very similar to the previously sequenced UCYN-A1 and UCYN-A2 sublineages, there are some differential genomic features such as the presence of genes for cell shape and cell wall biogenesis, as well as genes implicated in pyrimidine metabolism that are only present in UCYN-A2 and UCYN-A3. Also, comparative genomics suggests that the three sublineages form a monophyletic group that diverged from a common ancestor with a reduced genome, with UCYN-A2 being the closest to UCYN-A3 and UCYN-A1 its relative. Finally, phylogenomic and molecular clock analyses were also performed to estimate the age of divergence of the UCYN-A1, UCYN-A2 and UCYN-A3 sublineages both within the UCYN-A group and in a broader phylogenetic context, thus providing a paleo-oceanographic context for when these UCYN-A sublineages originated Peer reviewed Conference Object South Atlantic Ocean Digital.CSIC (Spanish National Research Council) |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Conserve and sustainably use the oceans seas and marine resources for sustainable development |
spellingShingle |
Conserve and sustainably use the oceans seas and marine resources for sustainable development Vieyra Mexicano, Cinthya Bianchini, Giorgio Garczarek, Laurence Wincker, Patrick Acinas, Silvia G. Sánchez-Baracaldo, Patricia Zehr, Jonathan P. Cornejo-Castillo, Francisco M. Single-cell genomics reveals the genome content of the uncultured cyanobacterial diazotroph UCYN-A3 |
topic_facet |
Conserve and sustainably use the oceans seas and marine resources for sustainable development |
description |
ASLO Aquatic Sciences Meeting, Resilience and Recovery in Aquatic Systems, 4-9 June 2023, Palma de Mallorca, Spain The unicellular cyanobacteria Candidatus Atelocyanobacterium thalassa (hereafter, UCYN-A) are important nitrogen fixers that live in symbiosis with the haptophyte algae Braarudosphaera bigelowii and its close relative species. Although the UCYN-A phylogenetic group includes at least six sublineages, only the genomes of UCYN-A1 and UCYN-A2 had been sequenced to date. Therefore, despite its biogeochemical relevance, comparative genomics on the UCYN-A phylogenetic group has been limited by the poor representation of genomic data. Here, using a high-throughput DNA sequencing approach on a single sorted cell from the South Atlantic Ocean, we reconstructed about 65% of the genome of the UCYN-A3 sublineage. We show that although the genomic content of UCYN-A3 is very similar to the previously sequenced UCYN-A1 and UCYN-A2 sublineages, there are some differential genomic features such as the presence of genes for cell shape and cell wall biogenesis, as well as genes implicated in pyrimidine metabolism that are only present in UCYN-A2 and UCYN-A3. Also, comparative genomics suggests that the three sublineages form a monophyletic group that diverged from a common ancestor with a reduced genome, with UCYN-A2 being the closest to UCYN-A3 and UCYN-A1 its relative. Finally, phylogenomic and molecular clock analyses were also performed to estimate the age of divergence of the UCYN-A1, UCYN-A2 and UCYN-A3 sublineages both within the UCYN-A group and in a broader phylogenetic context, thus providing a paleo-oceanographic context for when these UCYN-A sublineages originated Peer reviewed |
format |
Conference Object |
author |
Vieyra Mexicano, Cinthya Bianchini, Giorgio Garczarek, Laurence Wincker, Patrick Acinas, Silvia G. Sánchez-Baracaldo, Patricia Zehr, Jonathan P. Cornejo-Castillo, Francisco M. |
author_facet |
Vieyra Mexicano, Cinthya Bianchini, Giorgio Garczarek, Laurence Wincker, Patrick Acinas, Silvia G. Sánchez-Baracaldo, Patricia Zehr, Jonathan P. Cornejo-Castillo, Francisco M. |
author_sort |
Vieyra Mexicano, Cinthya |
title |
Single-cell genomics reveals the genome content of the uncultured cyanobacterial diazotroph UCYN-A3 |
title_short |
Single-cell genomics reveals the genome content of the uncultured cyanobacterial diazotroph UCYN-A3 |
title_full |
Single-cell genomics reveals the genome content of the uncultured cyanobacterial diazotroph UCYN-A3 |
title_fullStr |
Single-cell genomics reveals the genome content of the uncultured cyanobacterial diazotroph UCYN-A3 |
title_full_unstemmed |
Single-cell genomics reveals the genome content of the uncultured cyanobacterial diazotroph UCYN-A3 |
title_sort |
single-cell genomics reveals the genome content of the uncultured cyanobacterial diazotroph ucyn-a3 |
publisher |
Association for the Sciences of Limnology and Oceanography |
publishDate |
2023 |
url |
http://hdl.handle.net/10261/354350 |
genre |
South Atlantic Ocean |
genre_facet |
South Atlantic Ocean |
op_relation |
Sí ASLO Aquatic Sciences Meeting (2023) http://hdl.handle.net/10261/354350 |
op_rights |
none |
_version_ |
1799466857086844928 |