Use of lectins to characterise genetic variability and geographic differentiation in natural populations of Thunnus alalunga (Bonn. 1788)
New tools from cell and molecular biology can be used to implement the knowledge of fish biology and fisheries. In this sense, we suggest that the use of lectins can be an understandable procedure to study population structure of fishes. Lectins are proteins or glycoproteins of nonimmune origin that...
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International Commission for the Conservation of Atlantic Tunas (ICCAT)
2002
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ftcsic:oai:digital.csic.es:10261/328349 2024-02-11T10:06:20+01:00 Use of lectins to characterise genetic variability and geographic differentiation in natural populations of Thunnus alalunga (Bonn. 1788) López-Rodas, Victoria Arrizabalaga, Haritz Nieto, Blanca González-Garcés-Santiso, Alberto Costas, Eduardo Atlantic Ocean Central Atlantic Eastern Central Atlantic Guinea waters 2002 http://hdl.handle.net/10508/1660 http://hdl.handle.net/10261/328349 en eng International Commission for the Conservation of Atlantic Tunas (ICCAT) Centro Oceanográfico de Vigo López-Rodas, V., H. Arrizabalaga, B. Nieto, A. González-Garcés, E. Costas. 2002. Use of lectins to characterise genetic variability and geographic differentiation in natural populations of Thunnus alalunga (Bonn. 1788). Col. Vol. Sci. Pap. ICCAT, 54 (5): 1495-1507. http://hdl.handle.net/10508/1660 http://hdl.handle.net/10261/328349 open Centro Oceanográfico de Vigo Pesquerías documento de trabajo http://purl.org/coar/resource_type/c_8042 2002 ftcsic 2024-01-16T11:49:20Z New tools from cell and molecular biology can be used to implement the knowledge of fish biology and fisheries. In this sense, we suggest that the use of lectins can be an understandable procedure to study population structure of fishes. Lectins are proteins or glycoproteins of nonimmune origin that agglutinate cells and/or precipitate complex carbohydrates. Several lectins behave like blood group antibodies and can be used as specific gene markers. We characterise blood groups of Thunnus alalunga worldwide populations under a population genetics point of view using lectins. Fishes from several populations of Mediterranean Sea, Atlantic, Pacific and Indian Oceans were captured and 5 mL blood was obtained from each fish. Each fish was treated with 8 FITC-labelled lectins. The binding activity of the lectins was detected using a fluorescence assay. Several statistical procedures of populations genetics were employed to analyse genetic variability, geographic differentiation, and genetic distance showing that: i) there is genetic variability within Thunnus alalunga populations, ii) we detect highly significant heterogeneity among populations, and considerable geographic differentiation, iii) Mediterranean population is quite distant of North Atlantic population, iv) North and South Atlantic populations are extremely distant, v) Pacific population is the most distant from the others , vi) Gulf of Guinea and South Atlantic populations are distant, vii) Gulf of Guinea and North Atlantic are proximate, viii) Indian and South Atlantic populations are also proximate. De nouveaux moyens fournis par la biologie cellulaire et moléculaire peuvent servir à appliquer les connaissances sur la biologie et la pêche du poisson. A cet égard, nous suggérons que l'utilisation des lectines peut constituer un processus raisonnable pour étudier la structure des stocks de poisson. Les lectines sont des protéines ou des glycoprotéines d'origine non-immunitaire qui agglutinent les cellules et/ou précipitent les carbohydrates complexes. ... Report North Atlantic Digital.CSIC (Spanish National Research Council) Indian Pacific |
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Digital.CSIC (Spanish National Research Council) |
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ftcsic |
language |
English |
topic |
Centro Oceanográfico de Vigo Pesquerías |
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Centro Oceanográfico de Vigo Pesquerías López-Rodas, Victoria Arrizabalaga, Haritz Nieto, Blanca González-Garcés-Santiso, Alberto Costas, Eduardo Use of lectins to characterise genetic variability and geographic differentiation in natural populations of Thunnus alalunga (Bonn. 1788) |
topic_facet |
Centro Oceanográfico de Vigo Pesquerías |
description |
New tools from cell and molecular biology can be used to implement the knowledge of fish biology and fisheries. In this sense, we suggest that the use of lectins can be an understandable procedure to study population structure of fishes. Lectins are proteins or glycoproteins of nonimmune origin that agglutinate cells and/or precipitate complex carbohydrates. Several lectins behave like blood group antibodies and can be used as specific gene markers. We characterise blood groups of Thunnus alalunga worldwide populations under a population genetics point of view using lectins. Fishes from several populations of Mediterranean Sea, Atlantic, Pacific and Indian Oceans were captured and 5 mL blood was obtained from each fish. Each fish was treated with 8 FITC-labelled lectins. The binding activity of the lectins was detected using a fluorescence assay. Several statistical procedures of populations genetics were employed to analyse genetic variability, geographic differentiation, and genetic distance showing that: i) there is genetic variability within Thunnus alalunga populations, ii) we detect highly significant heterogeneity among populations, and considerable geographic differentiation, iii) Mediterranean population is quite distant of North Atlantic population, iv) North and South Atlantic populations are extremely distant, v) Pacific population is the most distant from the others , vi) Gulf of Guinea and South Atlantic populations are distant, vii) Gulf of Guinea and North Atlantic are proximate, viii) Indian and South Atlantic populations are also proximate. De nouveaux moyens fournis par la biologie cellulaire et moléculaire peuvent servir à appliquer les connaissances sur la biologie et la pêche du poisson. A cet égard, nous suggérons que l'utilisation des lectines peut constituer un processus raisonnable pour étudier la structure des stocks de poisson. Les lectines sont des protéines ou des glycoprotéines d'origine non-immunitaire qui agglutinent les cellules et/ou précipitent les carbohydrates complexes. ... |
format |
Report |
author |
López-Rodas, Victoria Arrizabalaga, Haritz Nieto, Blanca González-Garcés-Santiso, Alberto Costas, Eduardo |
author_facet |
López-Rodas, Victoria Arrizabalaga, Haritz Nieto, Blanca González-Garcés-Santiso, Alberto Costas, Eduardo |
author_sort |
López-Rodas, Victoria |
title |
Use of lectins to characterise genetic variability and geographic differentiation in natural populations of Thunnus alalunga (Bonn. 1788) |
title_short |
Use of lectins to characterise genetic variability and geographic differentiation in natural populations of Thunnus alalunga (Bonn. 1788) |
title_full |
Use of lectins to characterise genetic variability and geographic differentiation in natural populations of Thunnus alalunga (Bonn. 1788) |
title_fullStr |
Use of lectins to characterise genetic variability and geographic differentiation in natural populations of Thunnus alalunga (Bonn. 1788) |
title_full_unstemmed |
Use of lectins to characterise genetic variability and geographic differentiation in natural populations of Thunnus alalunga (Bonn. 1788) |
title_sort |
use of lectins to characterise genetic variability and geographic differentiation in natural populations of thunnus alalunga (bonn. 1788) |
publisher |
International Commission for the Conservation of Atlantic Tunas (ICCAT) |
publishDate |
2002 |
url |
http://hdl.handle.net/10508/1660 http://hdl.handle.net/10261/328349 |
op_coverage |
Atlantic Ocean Central Atlantic Eastern Central Atlantic Guinea waters |
geographic |
Indian Pacific |
geographic_facet |
Indian Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
Centro Oceanográfico de Vigo López-Rodas, V., H. Arrizabalaga, B. Nieto, A. González-Garcés, E. Costas. 2002. Use of lectins to characterise genetic variability and geographic differentiation in natural populations of Thunnus alalunga (Bonn. 1788). Col. Vol. Sci. Pap. ICCAT, 54 (5): 1495-1507. http://hdl.handle.net/10508/1660 http://hdl.handle.net/10261/328349 |
op_rights |
open |
_version_ |
1790603987635929088 |