Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters
The diversity of Alexandrium spp. in Irish coastal waters was investigated through the morphological examination of resting cysts and vegetative cells, the determination of PSP toxin and spirolide profiles and the sequence analysis of rDNA genes. Six morphospecies were characterised: A. tamarense, A...
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ftcsic:oai:digital.csic.es:10261/323433 2024-02-11T10:07:05+01:00 Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters Touzet, Nicolás Franco, José Mariano Raine, Robin Atlantic Ocean Eastern Atlantic Northeast Atlantic Irish Sea 2008-10 http://hdl.handle.net/10508/9907 http://hdl.handle.net/10261/323433 https://doi.org/10.1016/j.hal.2008.04.001 en eng Centro Oceanográfico de Vigo VoR http://www.sciencedirect.com/science/article/pii/S1568988308000292 Harmful Algae, 7(6). 2008: 782-797 1568-9883 http://hdl.handle.net/10508/9907 http://hdl.handle.net/10261/323433 doi:10.1016/j.hal.2008.04.001 21543 open Medio Marino Alexandrium Centro Oceanográfico de Vigo HAB PSP toxins rDNA Resting cysts Spirolides research article 2008 ftcsic https://doi.org/10.1016/j.hal.2008.04.001 2024-01-16T11:47:09Z The diversity of Alexandrium spp. in Irish coastal waters was investigated through the morphological examination of resting cysts and vegetative cells, the determination of PSP toxin and spirolide profiles and the sequence analysis of rDNA genes. Six morphospecies were characterised: A. tamarense, A. minutum, A. ostenfeldii, A. peruvianum, A. tamutum and A. andersoni. Both PSP toxin producing and non-toxic strains of A. tamarense and A. minutum were observed. The average toxicities of toxic strains for both cultured species were respectively 11.3 (8.6 S.D.) and 2.3 (0.5 S.D.) pg STX equiv. cell−1. Alexandrium ostenfeldii and A. peruvianum did not synthesise PSP toxins but HPLC–MS analysis of two strains showed distinct spirolide profiles. A cyst-derived culture of A. peruvianum from Lough Swilly mainly produced spirolides 13 desmethyl-C and 13 desmethyl-D whereas one of A. ostenfeldii, from Bantry Bay, produced spirolides C and D. Species identification was confirmed through the analyses of SSU, ITS1-5.8S-ITS2 and LSU rDNA genes. Some nucleotide variability was observed among clones of toxic strains of A. tamarense, which all clustered within the North American clade. However, rDNA sequencing did not allow discrimination between the toxic and non-toxic forms of A. minutum. Phylogenetic analysis also permitted the differentiation of A. ostenfeldii from A. peruvianum. Resting cysts of PSP toxin producing Alexandrium species were found in Cork Harbour and Belfast Lough, locations where shellfish contamination events have occurred in the past, highlighting the potential for the initiation of harmful blooms from cyst beds. The finding of supposedly non-toxic and biotoxin-producing Alexandrium species near aquaculture production sites will necessitate the use of reliable discriminative methods in phytoplankton monitoring. SI Article in Journal/Newspaper Northeast Atlantic Digital.CSIC (Spanish National Research Council) Harmful Algae 7 6 782 797 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Medio Marino Alexandrium Centro Oceanográfico de Vigo HAB PSP toxins rDNA Resting cysts Spirolides |
spellingShingle |
Medio Marino Alexandrium Centro Oceanográfico de Vigo HAB PSP toxins rDNA Resting cysts Spirolides Touzet, Nicolás Franco, José Mariano Raine, Robin Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters |
topic_facet |
Medio Marino Alexandrium Centro Oceanográfico de Vigo HAB PSP toxins rDNA Resting cysts Spirolides |
description |
The diversity of Alexandrium spp. in Irish coastal waters was investigated through the morphological examination of resting cysts and vegetative cells, the determination of PSP toxin and spirolide profiles and the sequence analysis of rDNA genes. Six morphospecies were characterised: A. tamarense, A. minutum, A. ostenfeldii, A. peruvianum, A. tamutum and A. andersoni. Both PSP toxin producing and non-toxic strains of A. tamarense and A. minutum were observed. The average toxicities of toxic strains for both cultured species were respectively 11.3 (8.6 S.D.) and 2.3 (0.5 S.D.) pg STX equiv. cell−1. Alexandrium ostenfeldii and A. peruvianum did not synthesise PSP toxins but HPLC–MS analysis of two strains showed distinct spirolide profiles. A cyst-derived culture of A. peruvianum from Lough Swilly mainly produced spirolides 13 desmethyl-C and 13 desmethyl-D whereas one of A. ostenfeldii, from Bantry Bay, produced spirolides C and D. Species identification was confirmed through the analyses of SSU, ITS1-5.8S-ITS2 and LSU rDNA genes. Some nucleotide variability was observed among clones of toxic strains of A. tamarense, which all clustered within the North American clade. However, rDNA sequencing did not allow discrimination between the toxic and non-toxic forms of A. minutum. Phylogenetic analysis also permitted the differentiation of A. ostenfeldii from A. peruvianum. Resting cysts of PSP toxin producing Alexandrium species were found in Cork Harbour and Belfast Lough, locations where shellfish contamination events have occurred in the past, highlighting the potential for the initiation of harmful blooms from cyst beds. The finding of supposedly non-toxic and biotoxin-producing Alexandrium species near aquaculture production sites will necessitate the use of reliable discriminative methods in phytoplankton monitoring. SI |
format |
Article in Journal/Newspaper |
author |
Touzet, Nicolás Franco, José Mariano Raine, Robin |
author_facet |
Touzet, Nicolás Franco, José Mariano Raine, Robin |
author_sort |
Touzet, Nicolás |
title |
Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters |
title_short |
Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters |
title_full |
Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters |
title_fullStr |
Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters |
title_full_unstemmed |
Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters |
title_sort |
morphogenetic diversity and biotoxin composition of alexandrium (dinophyceae) in irish coastal waters |
publishDate |
2008 |
url |
http://hdl.handle.net/10508/9907 http://hdl.handle.net/10261/323433 https://doi.org/10.1016/j.hal.2008.04.001 |
op_coverage |
Atlantic Ocean Eastern Atlantic Northeast Atlantic Irish Sea |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_relation |
Centro Oceanográfico de Vigo VoR http://www.sciencedirect.com/science/article/pii/S1568988308000292 Harmful Algae, 7(6). 2008: 782-797 1568-9883 http://hdl.handle.net/10508/9907 http://hdl.handle.net/10261/323433 doi:10.1016/j.hal.2008.04.001 21543 |
op_rights |
open |
op_doi |
https://doi.org/10.1016/j.hal.2008.04.001 |
container_title |
Harmful Algae |
container_volume |
7 |
container_issue |
6 |
container_start_page |
782 |
op_container_end_page |
797 |
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1790605212664201216 |