Viral populations in the global deep ocean conveyor belt system assessed by targeted viromics
Viruses are an abundant, diverse and dynamic component of marine and terrestrial ecosystems. In the ocean, viruses play a key role in the biogeochemical cycles and controlling microbial abundance, diversity and evolution. Recent metagenomics studies assessed the structure of the viral community in t...
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ftcsic:oai:digital.csic.es:10261/318611 2024-02-11T09:58:20+01:00 Viral populations in the global deep ocean conveyor belt system assessed by targeted viromics De Corte, Daniele Martínez-Martínez, J. Cretoiu, S. Takaki, Y. Nunoura, T. Sintes, Eva Herndl, G.J. Yokokawa, T. 2019 http://hdl.handle.net/10508/14842 http://hdl.handle.net/10261/318611 https://www.frontiersin.org/articles/10.3389/fmicb.2019.01801/pdf https://doi.org/10.3389/fmicb.2019.01801 en eng Frontiers Media SA Centro Oceanográfico de Baleares https://www.frontiersin.org/articles/10.3389/fmicb.2019.01801/pdf http://hdl.handle.net/10508/14842 http://hdl.handle.net/10261/318611 doi:10.3389/fmicb.2019.01801 31496997 25105 open Centro Oceanográfico de Baleares Medio Marino y Protección Ambiental targeted viromics deep ocean circulation viruses deep ocean next generation sequencing artículo http://purl.org/coar/resource_type/c_6501 2019 ftcsic https://doi.org/10.3389/fmicb.2019.01801 2024-01-16T11:45:09Z Viruses are an abundant, diverse and dynamic component of marine and terrestrial ecosystems. In the ocean, viruses play a key role in the biogeochemical cycles and controlling microbial abundance, diversity and evolution. Recent metagenomics studies assessed the structure of the viral community in the upper ocean. However, little is known about the compositional changes in viral communities along the deep ocean conveyor belt. To assess potential changes in the viral community in the global deepwater circulation system, water samples were collected in the core of the North Atlantic Deep Water (NADW) (∼2,500 m) and Pacific Antarctic Bottom Water (∼4,000 m). Microbial and viral abundance were evaluated by flow cytometry. Subsequently, flow cytometry was used to sort virus-like particles and next generation sequencing was applied to build DNA libraries from the sorted virus populations. The viral communities were highly diverse across different oceanic regions with high dissimilarity between samples. Only 18% of the viral protein clusters were shared between the NADW and the Pacific Antarctic Bottom Water. Few viral groups, mainly associated with uncultured environmental and uncultured Mediterranean viruses were ubiquitously distributed along the global deep-water circulation system. Thus, our results point to a few groups of widely distributed abundant viruses in addition to the presence of rare and diverse types of viruses at a local scale. Sí Article in Journal/Newspaper Antarc* Antarctic NADW North Atlantic Deep Water North Atlantic Digital.CSIC (Spanish National Research Council) Antarctic Pacific Frontiers in Microbiology 10 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Centro Oceanográfico de Baleares Medio Marino y Protección Ambiental targeted viromics deep ocean circulation viruses deep ocean next generation sequencing |
spellingShingle |
Centro Oceanográfico de Baleares Medio Marino y Protección Ambiental targeted viromics deep ocean circulation viruses deep ocean next generation sequencing De Corte, Daniele Martínez-Martínez, J. Cretoiu, S. Takaki, Y. Nunoura, T. Sintes, Eva Herndl, G.J. Yokokawa, T. Viral populations in the global deep ocean conveyor belt system assessed by targeted viromics |
topic_facet |
Centro Oceanográfico de Baleares Medio Marino y Protección Ambiental targeted viromics deep ocean circulation viruses deep ocean next generation sequencing |
description |
Viruses are an abundant, diverse and dynamic component of marine and terrestrial ecosystems. In the ocean, viruses play a key role in the biogeochemical cycles and controlling microbial abundance, diversity and evolution. Recent metagenomics studies assessed the structure of the viral community in the upper ocean. However, little is known about the compositional changes in viral communities along the deep ocean conveyor belt. To assess potential changes in the viral community in the global deepwater circulation system, water samples were collected in the core of the North Atlantic Deep Water (NADW) (∼2,500 m) and Pacific Antarctic Bottom Water (∼4,000 m). Microbial and viral abundance were evaluated by flow cytometry. Subsequently, flow cytometry was used to sort virus-like particles and next generation sequencing was applied to build DNA libraries from the sorted virus populations. The viral communities were highly diverse across different oceanic regions with high dissimilarity between samples. Only 18% of the viral protein clusters were shared between the NADW and the Pacific Antarctic Bottom Water. Few viral groups, mainly associated with uncultured environmental and uncultured Mediterranean viruses were ubiquitously distributed along the global deep-water circulation system. Thus, our results point to a few groups of widely distributed abundant viruses in addition to the presence of rare and diverse types of viruses at a local scale. Sí |
format |
Article in Journal/Newspaper |
author |
De Corte, Daniele Martínez-Martínez, J. Cretoiu, S. Takaki, Y. Nunoura, T. Sintes, Eva Herndl, G.J. Yokokawa, T. |
author_facet |
De Corte, Daniele Martínez-Martínez, J. Cretoiu, S. Takaki, Y. Nunoura, T. Sintes, Eva Herndl, G.J. Yokokawa, T. |
author_sort |
De Corte, Daniele |
title |
Viral populations in the global deep ocean conveyor belt system assessed by targeted viromics |
title_short |
Viral populations in the global deep ocean conveyor belt system assessed by targeted viromics |
title_full |
Viral populations in the global deep ocean conveyor belt system assessed by targeted viromics |
title_fullStr |
Viral populations in the global deep ocean conveyor belt system assessed by targeted viromics |
title_full_unstemmed |
Viral populations in the global deep ocean conveyor belt system assessed by targeted viromics |
title_sort |
viral populations in the global deep ocean conveyor belt system assessed by targeted viromics |
publisher |
Frontiers Media SA |
publishDate |
2019 |
url |
http://hdl.handle.net/10508/14842 http://hdl.handle.net/10261/318611 https://www.frontiersin.org/articles/10.3389/fmicb.2019.01801/pdf https://doi.org/10.3389/fmicb.2019.01801 |
geographic |
Antarctic Pacific |
geographic_facet |
Antarctic Pacific |
genre |
Antarc* Antarctic NADW North Atlantic Deep Water North Atlantic |
genre_facet |
Antarc* Antarctic NADW North Atlantic Deep Water North Atlantic |
op_relation |
Centro Oceanográfico de Baleares https://www.frontiersin.org/articles/10.3389/fmicb.2019.01801/pdf http://hdl.handle.net/10508/14842 http://hdl.handle.net/10261/318611 doi:10.3389/fmicb.2019.01801 31496997 25105 |
op_rights |
open |
op_doi |
https://doi.org/10.3389/fmicb.2019.01801 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
_version_ |
1790593957441306624 |